Minor iridoids from <i>Scutellaria albida ssp. albida</i>. Inhibitory potencies on lipoxygenase, linoleic acid lipid peroxidation and antioxidant activity of iridoids from <i>Scutellaria sp</i>.

Gousiadou, Chrysoula; Gotfredsen, Charlotte Held; Matsa, Marina; Hadjipavlou‐Litina, Dimitra; Skaltsa, Helen

doi:10.3109/14756366.2012.672415

Cited by 19 publications

(11 citation statements)

References 33 publications

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“…While compound 4 was not tested due to limited amount, compounds 1 –3 displayed week activity in the range of 0.1–0.3 min/μM, compared to standard Trolox (14.12 min/μM) ( Table 4 and Figures 3 , 4 ). Our findings were in agreement with week activities reported previously for mussaenoside and mussaenosidic acid (Gousiadou et al, 2013). Isolated flavonoids ( 5–6 ) only weakly active in the range of 0.2–0.3 min/μM, compared to standard Trolox (14.12 min/μM) ( Table 4 and Figures 3 , 4 ).…”

Section: Resultssupporting

confidence: 94%

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In Vitro and In Silico Antidiabetic and Antimicrobial Evaluation of Constituents from Kickxia ramosissima (Nanorrhinum ramosissimum)

et al. 2017

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Background and Aims: Kickxia ramosissima (Wall.) Janch (or Nanorrhinum ramosissimum (Wall.) Betsche is a well-known medicinal plant in Pakistan that is traditionally used in diabetic and inflammatory conditions. Because little information is available on its phytochemical composition, a range of constituents were isolated and evaluated in vitro in assays related to the traditional use.Methods: Dried whole plant material was extracted and chromatographically fractionated. Isolated constituents were evaluated in silico and in vitro in assays related to the traditional use against diabetes (inhibition of α-glucosidase activity; inhibition of advanced glycation endproducts) and in inflammatory conditions (inhibition of AAPH induced linoleic acid peroxidation, inhibition of 15-LOX, antimicrobial activity).Results: Phytochemical analysis of the extracts and fractions led to isolation of 7 compounds, including the iridoids kickxiasine (being a new compound), mussaenosidic acid, mussaenoside and linarioside; the flavonoids pectolinarigenin and pectolinarin; and 4-hydroxy-benzoic acid methyl ester. The iridoids showed weak antiglycation activity. The flavonoids, however, showed interesting results as pectolinarigenin was highly active compared to pectolinarin. In the α-glucosidase inhibition assay, only weak activity was observed for the iridoids. However, the flavonoid pectolinarigenin showed good activity, followed by pectolinarin. In the 15-LOX experiment, moderate inhibition was recorded for most compounds, the iridoids mussaenosidic acid and mussaenoside being the most active. In the AAPH assay, weak or no inhibition was recorded for all compounds. The in silico assays for the α-glucosidase and 15-LOX assays confirmed the results of respective in vitro assays. Pectolinarigenin showed moderate antimicrobial activity Kickxia Ramosissimaagainst Staphylococcus aureus, Plasmodium falciparum K1, and Trypanosoma cruzi, but it was not cytotoxic on a human MRC-5 cell line.Conclusion: Our findings may in part contribute to explain the traditional use of K. ramosissima.

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Section: Resultssupporting

confidence: 94%

“…Iridoids are particularly known for their anti-inflammatory activities (Park et al, 2010; Gousiadou et al, 2013). As the major constituents of Kickxia were iridoids, it was therefore considered interesting to test the isolated iridoids for inhibition of their 15-LOX potential.…”

Section: Resultsmentioning

confidence: 99%

In Vitro and In Silico Antidiabetic and Antimicrobial Evaluation of Constituents from Kickxia ramosissima (Nanorrhinum ramosissimum)

et al. 2017

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“…The number of hydroxyl groups on the aromatic moiety did not increase activity regardless of the presence of free hydroxyl groups on the main iridoid cores. Non-rigidity could exhibit a moderate interaction potential with DPPH [37][38][39][40]. Other results suggest that the double bond in the iridoid skeleton did not increase the antioxidant activity of compounds [25].…”

Section: Resultsmentioning

confidence: 99%

In vitro and in silico approach to determine neuroprotective properties of iridoid glycosides from aerial parts of Scrophularia amplexicaulis by investigating their cholinesterase inhibition and anti-oxidant activities

2020

Biointerface Res Appl Chem

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Three iridoid glycosides included 6-O-methyl, 1-glucopyranosyl catalpol (Compound 1), 6-O-α-L (3"-O- trans, 4"-O- trans cinnamoyl)-rhamnopyranosyl catalpol (Compound 2) and scropolioside D (Compound 3) were isolated from aerial parts of S. amplexicaulis using chromatographic methods. The structures were determined by different spectroscopic data. The inhibitory effects (IC50 values) of the compounds on cholinesterase (AChE and BChE) was determined by in-vitro assays. Docking studied were performed to investigate receptor-ligands interactions. The antioxidant capacity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, 2, 2'-azino-bis[3-ethylbenzothiazoline]-6-sulphonic acid (ABTS) radical cation, cupric ion reducing activity (CUPRAC), ferric reducing antioxidant power (FRAP), phosphomolybdenum and metal chelating assays. AChE and BChE were moderately inhibited by all of the investigated iridoid glycosides (compared to galantamine). Compound 2 and compound 3 showed comparable anti-oxidant effects with the Trolox as the control in phosphomolybdenum assay. Also, compound 1, showed acceptable activities in ABTS radical scavenging and Phosphomolybdenum assays compared to the control.

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“…The potential of T. portulacastum hydrolysates to inhibit the peroxidation of linoleic acid in vitro was assessed using the thiocyanate method (14). Linoleic acid, a poly-unsaturated fatty acid, is highly susceptible to autoxidation, which leads to the formation of peroxides.…”

Section: Resultsmentioning

confidence: 99%

“…Briefly, 5 mg of each hydrolysate was diluted with 10 mL of 0.2 M sodium phosphate buffer (pH 7) and mixed with a solution of linoleic acid (0.13 mL) and ethanol (10 mL). The mixture was diluted with distilled water to 25 mL and incubated at 40°C for 96 h. The thiocyanate method was used to measure the degree of oxidation (14). Briefly, 10 mL of ethanol (75% v/v), 0.2 mL of an aqueous solution of ammonium thiocyanate (30% w/v), and 0.2 mL of ferrous chloride (FeCl 2 ) solution (20 mM in 3.5% HCl) were added sequentially to 0.2 mL of sample.…”

Section: Methodsmentioning

confidence: 99%

In vitro Antioxidant Activities of Trianthema portulacastrum L. Hydrolysates

Yaqoob¹,

Sultana²,

Mushtaq³

2014

JFN

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Hydrolysates of Trianthema portulacastrum in acidified methanol were evaluated for their total phenolic (TP) constituents and respective antioxidant activities using in vitro assays (i.e., 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, percent inhibition of linoleic acid peroxidation, and ferric reducing power). The observed results indicate that root, shoot, and leaf fractions of T. portulacastrum contain 50.75~98.09 mg gallic acid equivalents/g dry weight of TP. In addition, these fractions have substantial reducing potentials (0.10~0.59), abilities to inhibit peroxidation (43.26~89.98%), and DPPH radical scavenging capabilities (6.98~311.61 μg/mL IC50). The experimental data not only reveal T. portulacastrum as potential source of valuable antioxidants, but also indicate that acidified methanol may be an ideal choice for the enhanced recovery of phenolic compounds with retained biological potential for the food and pharmaceutical industry.

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Minor iridoids from Scutellaria albida ssp. albida. Inhibitory potencies on lipoxygenase, linoleic acid lipid peroxidation and antioxidant activity of iridoids from Scutellaria sp.

Cited by 19 publications

References 33 publications

In Vitro and In Silico Antidiabetic and Antimicrobial Evaluation of Constituents from Kickxia ramosissima (Nanorrhinum ramosissimum)

In Vitro and In Silico Antidiabetic and Antimicrobial Evaluation of Constituents from Kickxia ramosissima (Nanorrhinum ramosissimum)

In vitro and in silico approach to determine neuroprotective properties of iridoid glycosides from aerial parts of Scrophularia amplexicaulis by investigating their cholinesterase inhibition and anti-oxidant activities

In vitro Antioxidant Activities of Trianthema portulacastrum L. Hydrolysates

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