MIP-1α and MCP-1 as salivary biomarkers in periodontal disease

Nisha, KJ; Suresh, Aparnna; Anilkumar, Ananyaveena; Padmanabhan, Shyam

doi:10.1016/j.sdentj.2018.07.002

Cited by 30 publications

(29 citation statements)

References 31 publications

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“…Similarly, eotaxin‐3 (CCL26) aids in the recruitment of both basophils and eosinophils 69 causing excessive production of cytotoxic ECPs 70 consequently resulting in degradation of the periodontium 71 . The elevation in macrophage inflammatory proteins (MIP‐1α and MIP‐1β) in IL‐1β stimulated hPDLFs correlates positively with increased expression of MIP‐1α in the saliva of PD patients 72 which are absent in gingival tissues of healthy individuals 73 . Furthermore, the increase in IFN‐γ may be detrimental in PD as increased IFN‐γ levels are implicated in progressive, chronic periodontal lesions 74 .…”

Section: Discussionmentioning

confidence: 99%

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Abidi

Alghamdi

Dabbous

et al. 2020

J of Periodontal Research

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Objective The aim of this study is to understand the role of cannabinoid type 2 receptor (CB2R) during periodontal inflammation and to identify anti‐inflammatory agents for the development of drugs to treat periodontitis (PD). Background Cannabinoid type 2 receptor is found in periodontal tissue at sites of inflammation/infection. Our previous study demonstrated anti‐inflammatory responses in human periodontal ligament fibroblasts (hPDLFs) via CB2R ligands. Methods Anandamide (AEA), HU‐308 (agonist), and SMM‐189 (inverse agonist) were tested for effects on IL‐1β‐stimulated cytokines, chemokines, and angiogenic and vascular markers expressed by hPDLFs using Mesoscale Discovery V‐Plex Kits. Signal transduction pathways (p‐c‐Jun, p‐ERK, p‐p‐38, p‐JNK, p‐CREB, and p‐NF‐kB) were investigated using Cisbio HTRF kits. ACTOne and Tango™ ‐BLA functional assays were used to measure cyclic AMP (cAMP) and β‐arrestin activity. Results IL‐1β stimulated hPDLF production of 18/39 analytes, which were downregulated by the CB2R agonist and the inverse agonist. AEA exhibited pro‐inflammatory and anti‐inflammatory effects. IL‐1β increased phosphoproteins within the first hour except p‐JNK. CB2R ligands attenuated p‐p38 and p‐NFĸB, but a late rise in p‐38 was seen with HU‐308. As p‐ERK levels declined, a significant increase in p‐ERK was observed later in the time course by synthetic CB2R ligands. P‐JNK was significantly affected by SMM‐189 only, while p‐CREB was elevated significantly by CB2R ligands at 180 minutes. HU‐308 affected both cAMP and β‐arrestin pathway. SMM‐189 only stimulated cAMP. Conclusion The findings that CB2R agonist and inverse agonist may potentially regulate inflammation suggest that development of CB2R therapeutics could improve on current treatments for PD and other oral inflammatory pathologies.

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Section: Discussionmentioning

confidence: 99%

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Abidi

Alghamdi

Dabbous

et al. 2020

J of Periodontal Research

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“…Increased concentrations of MCP-1, MCP-3, macrophage migration inhibitory factor (MIF), MIP-1α, IP-10 and macrophages have been demonstrated in inflamed gingival biopsies, as compared to biopsies from healthy gingival sites [7][8][9][10][11]. Moreover, increased concentrations of chemokines like MIP-1α in saliva have been reported to associate with periodontitis [12][13][14][15][16]. This suggests that concentrations of chemokines in saliva can reflect local periodontal inflammation.…”

Section: Introductionmentioning

confidence: 99%

Salivary concentrations of macrophage activation-related chemokines are influenced by non-surgical periodontal treatment: a 12-week follow-up study

Grande

Belstrøm

Damgaard

et al. 2019

Journal of Oral Microbiology

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Background: During periodontal inflammation, bacteria induces chemokine expression and migration of various inflammatory cells. The aim of the study was to learn if periodontal treatment alters salivary concentrations of macrophage activation-related chemokines and if such alterations correlate with abundance of periodontitis-associated bacteria.Methods: Twenty-five patients with periodontitis completed the study (NCT02913248 at clinicaltrials.gov). Periodontal parameters and stimulated saliva samples were obtained at baseline and 2, 6 and 12 weeks after non-surgical periodontal treatment. Salivary concentrations of monocyte chemoattractant proteins (MCP-1-4), macrophage-derived chemokine (MDC), macrophage migration inhibitory factor (MIF), monokine induced by interferon-gamma (MIG), macrophage inflammatory protein (MIP-1α) and interferon-inducible protein (IP-10) were quantified using the Luminex® xMAP™ technique and abundance of bacteria was quantified using next-generation sequencing.Results: The treatment improved all periodontal parameters and caused an increase in the concentrations of MCP-2, MDC and MIP-1α at week 12 compared to baseline, week 2 and week 6, respectively. Salivary concentrations of MCP-1-2, MDC, MIG, MIP-1α and IP-10 correlated with the abundance of specific periodontitis-associated bacteria.Conclusions: Periodontal treatment impacts salivary concentrations of MCP-2, MDC and MIP-1α, which correlate with the abundance of specific periodontitis-associated bacteria. This indicates that these chemokines reflect periodontal status and possess potential in illustrating a response to treatment.

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“…Regarding MCP-1, Gupta et al [54] conducted a study in 45 patients with an average age of 43 years for healthy patients and 41 for patients with periodontitis, with results similar to Nisha et al [55]. In both studies it was found that the levels of MCP-1 in saliva can be a good biomarker for the development of periodontal disease.…”

Section: Proteins (Cytokines) Determined In Saliva That Could Be Usedmentioning

confidence: 81%

Use of Biomarkers for the Diagnosis of Periodontitis

González-Ramírez¹,

Serafín-Higuera²,

Mancilla³

et al. 2020

Periodontal Disease - Diagnostic and Adjunctive Non-Surgical Considerations

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Periodontal disease is the most common oral condition of human population; if periodontitis is not treated in its initial stages, it can cause the loss of teeth. The diagnosis of periodontitis is based on clinical measurements. However, currently with the advancement of technology, other diagnostic and monitoring options are being search. In fact, different types of biomarkers have been evaluated where different biological fluids have been used as a source of the sample. We will try to summarize existing biomarkers of different periodontitis stages and make a comparison of the periodontal biomarkers evaluated so far and their usefulness in diagnosis and monitoring of periodontitis.

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MIP-1α and MCP-1 as salivary biomarkers in periodontal disease

Abstract: There is a substantial increase in the concentration of both MIP-1α and MCP-1 with increasing severity of periodontal disease. Both the analytes showed promising results as biomarkers for discriminating periodontal disease from health.

Cited by 30 publications

References 31 publications

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Salivary concentrations of macrophage activation-related chemokines are influenced by non-surgical periodontal treatment: a 12-week follow-up study

Use of Biomarkers for the Diagnosis of Periodontitis

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