miR‐10a‐5p is increased in atopic dermatitis and has capacity to inhibit keratinocyte proliferation

Vaher, Helen; Runnel, Toomas; Urgard, Egon; Aab, Alar; Carreras‐Badosa, Gemma; Maslovskaja, Julia; Abram, Kristi; Raam, Liisi; Kaldvee, Bret; Annilo, Tarmo; Tkaczyk, Eric R.; Maimets, Toivo; Akdiş, Cezmi A.; Kingo, Külli; Rebane, Ana

doi:10.1111/all.13849

Cited by 30 publications

(25 citation statements)

References 46 publications

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“…Additionally, miR-10a was found to inhibit cell proliferation by targeting BCL6 in diffuse large B-cell lymphoma [47]. MiR-10a-5p was proved to have the capacity to inhibit keratinocyte proliferation [48]. According to these reports, miR-148a and miR-10a can inhibit cell proliferation which is consistent with our results.…”

Section: Discussionsupporting

confidence: 91%

Preliminary study on microR-148a and microR-10a in dermal papilla cells of Hu sheep

Gao

Jin

et al. 2019

BMC Genet

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Background: Hu sheep, a unique Chinese breed with high reproductive performance, are also well known for their rare white lambskin in China. The quality of lambskin is affected by hair follicles, and dermal papilla cells are an important component of hair follicles that plays a key role in hair follicle growth and development. This study helps elucidate the effect of miR-148a and miR-10a on hair follicle growth and development. Results: Based on the results of gene chip and high-throughput sequencing, bone morphogenetic protein 7 (BMP7) was used as a research object. Bioinformatics analysis and the dual-luciferase reporter system indicated that, along with Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) that miR-148a and miR-10a target relationships with BMP7. BMP7 was the target gene both for miR-148a and miR-10a by the dual-luciferase reporter system and Western blot. Hu sheep dermal papilla cells were successfully isolated and purified, and after transfecting miR-148a/miR-10a mimics and inhibitors into dermal papilla cells, a Cell Counting Kit-8 (CCK-8) was used to determine that miR-148a/miR-10a inhibited the proliferation of Hu sheep dermal papilla cells. In addition, after the overexpression of miR-148a, the expression levels of Smad3 (P < 0.05), Smad6 (P < 0.05), Smad4 (P < 0.01), and Smad5 (P < 0.01) were significantly higher than those of the control groups. After the inhibition of miR-148a, the expression levels of Smad3 (P < 0.05), Smad4 (P < 0.05), and TGF-β (P < 0.01) were significantly lower than those of the control groups. After the overexpression of miR-10a, the expression levels of Smad1 (P < 0.01), Smad2 (P < 0.05), Smad4 (P < 0.01), Smad5 (P < 0.01), and TGF-β (P < 0.05) were significantly lower than those of the control groups. After the inhibition of miR-10a, the expression levels of Smad1 (P < 0.01) and Smad2 (P < 0.05) were significantly lower than those of the control groups. Conclusions: These results revealed the target relationship between miR-148a, miR-10a and BMP7, and the effect of miR-148a and miR-10a on the proliferation of dermal papilla cells. They will provide the basis for a follow-up study on how miR-148a, and miR-10a mediate BMP7 regulation of hair follicle growth and development.

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Section: Discussionsupporting

confidence: 91%

Preliminary study on microR-148a and microR-10a in dermal papilla cells of Hu sheep

Gao

Jin

et al. 2019

BMC Genet

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“…Egr-miR-10a-5p was predicted to target the NECTIN2, COLGALT1, COL5A2 , and DDTL genes, which were involved in the positive regulation of natural killer cell–mediated cytotoxicity and T-cell receptor signaling pathway, collagen fibril organization, collagen catabolic process, tumor necrosis factor production, and ERK1 and ERK2 cascades, respectively (Stanietsky et al, 2013 ; Park et al, 2017 ; Miyatake et al, 2018 ). It has been reported that hsa-miR-10a-5p had a role in regulating the proliferation and invasiveness of cancer cells and inflammatory responses in endothelial cells (Vaher et al, 2019 ). miR-10a-5p inhibited MAP3K7 and β-transducin repeat-containing gene expression, thereby reducing NF-κB activation and displaying anti-inflammatory effects in the athero-susceptible endothelium (Fang et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

“…High-throughput sequencing was conducted to analyze the ncRNAs (miRNAs, circRNAs, and lncRNAs) profiles in the two types of ELVs, and bioinformatics analysis of the 20 most abundant miRNAs in the two types of ELVs was performed to explore the possible biological processes and pathways associated with pathogenicity and the host immune response. These ELVs contain small RNA species, including specific miRNAs that are homologous to host miRNAs with known immunomodulatory roles (Zhang et al, 2016 ; Vaher et al, 2019 ). The results demonstrated that the most abundant miRNAs in both types of ELVs might be new candidates for the mechanism of parasite–parasite communication and parasite–host interactions of ELVs in parasitic diseases.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Non-coding RNA Profiles of Exosome-Like Vesicles From the Protoscoleces and Hydatid Cyst Fluid of Echinococcus granulosus

Zhang

Gong

Cao

et al. 2020

Front. Cell. Infect. Microbiol.

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Cystic echinococcosis is a worldwide chronic zoonotic disease that threatens human health and animal husbandry. Exosome-like vesicles (ELVs) have emerged recently as mediators in the parasite-parasite intercommunication and parasite-host interactions. Exosome-like vesicles from parasites can transfer non-coding RNAs (ncRNAs) into host cells to regulate their gene expression; however, the ncRNAs profiles of the ELVs from Echinococcus granulosus remain unknown. Here, we isolated protoscolece (PSC)-ELVs and hydatid fluid (HF)-ELVs from the culture medium for E. granulosus PSCs in vitro and the HF of fertile sheep cysts, respectively. The microRNA (miRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA) profiles of the two types of ELVs were analyzed using high-throughput sequencing, and their functions were predicted using Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis. In PSC-ELVs and HF-ELVs, 118 and 58 miRNAs were identified, respectively, among which 53 miRNAs were present in both ELVs, whereas 65 and 5 miRNAs were unique to PSC-ELVs and HF-ELVs, respectively; 2,361 and 1,254 lncRNAs were identified in PSC-ELVs and HF-ELVs, respectively, among which 1,004 lncRNAs were present in both ELVs, whereas 1,357 and 250 lncRNAs were unique to PSC-ELVs and HF-ELVs, respectively. Intriguingly, the spilled PSCs from cysts excrete ELVs with higher numbers of and higher expression levels of miRNAs and circRNAs than HF-ELVs. The miRNA sequencing data were validated by quantitative reverse transcription-polymerase chain reaction. Furthermore, the target lncRNAs and mRNAs regulated by the 20 most abundant miRNAs were screened, and a ceRNA regulatory network containing 5 miRNAs, 41 lncRNAs, and 23 mRNAs was constructed, which provided new ideas and the molecular basis for further clarification of the function and mechanism of E. granulosus ELVs ncRNAs in the parasite-host interactions. Egr-miR-125-5p and egr-miR-10a-5p, sharing identical seed sites with host miRNAs, were predicted to mediate inflammatory response, collagen catabolic process, and mitogen-activated Zhang et al. NcRNAs in Echinococcus granulosus ELVs protein kinase cascade during parasite infections. In conclusion, for the first time, we identified the ncRNAs profiles in PSC-ELVs and HF-ELVs that might be involved in host immunity and pathogenesis, and enriched the ncRNAs data of E. granulosus. These results provided valuable resources for further analysis of the regulatory potential of ncRNAs, especially miRNAs, in both types of ELVs at the parasite-host interface.

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“…In addition, CCL5 was identified as a novel, direct target of miR-146a [ 31 ]. It is worth mentioning that the upregulation of miR-10a-5p, miR-29b, miR-223 and miR-151a have also been described in the inflammatory response and keratinocyte apoptosis for AD patients [ 75 , 76 , 77 , 78 ] ( Table 1 ).…”

Section: Role Of Mirnas In the Skin Pathogenesis Of Cutaneous Immumentioning

confidence: 99%

MicroRNAs in Several Cutaneous Autoimmune Diseases: Psoriasis, Cutaneous Lupus Erythematosus and Atopic Dermatitis

Domingo

Solé

Moliné

et al. 2020

Cells

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MicroRNAs (miRNAs) are endogenous small non-coding RNA molecules that regulate the gene expression at a post-transcriptional level and participate in maintaining the correct cell homeostasis and functioning. Different specific profiles have been identified in lesional skin from autoimmune cutaneous diseases, and their deregulation cause aberrant control of biological pathways, contributing to pathogenic conditions. Detailed knowledge of microRNA-affected pathways is of crucial importance for understating their role in skin autoimmune diseases. They may be promising therapeutic targets with novel clinical implications. They are not only present in skin tissue, but they have also been found in other biological fluids, such as serum, plasma and urine from patients, and therefore, they are potential biomarkers for the diagnosis, prognosis and response to treatment. In this review, we discuss the current understanding of the role of described miRNAs in several cutaneous autoimmune diseases: psoriasis (Ps, 33 miRNAs), cutaneous lupus erythematosus (CLE, 2 miRNAs) and atopic dermatitis (AD, 8 miRNAs). We highlight their role as crucial elements implicated in disease pathogenesis and their applicability as biomarkers and as a novel therapeutic approach in the management of skin inflammatory diseases.

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miR‐10a‐5p is increased in atopic dermatitis and has capacity to inhibit keratinocyte proliferation

Cited by 30 publications

References 46 publications

Preliminary study on microR-148a and microR-10a in dermal papilla cells of Hu sheep

Preliminary study on microR-148a and microR-10a in dermal papilla cells of Hu sheep

Comprehensive Analysis of Non-coding RNA Profiles of Exosome-Like Vesicles From the Protoscoleces and Hydatid Cyst Fluid of Echinococcus granulosus

MicroRNAs in Several Cutaneous Autoimmune Diseases: Psoriasis, Cutaneous Lupus Erythematosus and Atopic Dermatitis

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