miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphoma

Fan, Qian; Meng, Xiangrui; Liang, Hongwei; Zhang, Huilai; Liu, Xianming; Li, Lanfang; Li, Wei; Sun, Wei; Zhang, Haiyang; Zen, Ke; Zhang, Chen Yu; Zhou, Zhen; Chen, Xi; Ba, Yi

doi:10.1007/s13238-016-0316-z

Cited by 44 publications

(40 citation statements)

References 45 publications

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“…expression of miRNAs has been found in several cancers, which play novel roles as anti-oncogenes or oncogenes, depending on the function of the target genes. [15][16][17] Previous studies showed that aberrant expression of many miRNAs promote proliferation and metastases in GC. [18][19][20] However, the molecular events are not fully understood.…”

Section: Aberrantmentioning

confidence: 99%

“…The biological functions of miRNAs that regulating gene expression include cell proliferation, cell apoptosis, stress tolerance, and fat metabolism . Aberrant expression of miRNAs has been found in several cancers, which play novel roles as anti‐oncogenes or oncogenes, depending on the function of the target genes . Previous studies showed that aberrant expression of many miRNAs promote proliferation and metastases in GC .…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

Zhang

Sun

et al. 2018

Cancer Science

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Gastric cancer (GC) is one of the most common malignancies worldwide and has high morbidity and mortality rates. It is essential to elucidate the molecular events of GC proliferation and invasion, which will provide new therapeutic targets for GC. The inactivation of transforming growth factor‐β receptor 2 (TGFβR2) correlates with cancer cell growth and metastasis, but the mechanisms underlying the downregulation of TGFβR2 expression remain unknown. MicroRNAs (miRNAs) act as post‐transcriptional regulators and play a key role in the development of cancers. Bioinformatics analysis and luciferase reporter assays have shown that miR‐155 directly binds to the 3′‐UTR of TGFβR2 mRNA. In this study, we found that the TGFβR2 protein levels, but not mRNA levels, were downregulated in GC tissues, and the levels of miR‐155 were significantly increased in GC tissues. We deduced that miR‐155 was inversely correlated with TGFβR2 in GC cells. In vitro studies showed that overexpression of miR‐155 in SGC7901 inhibited the expression of TGFβR2 and then promoted GC cell proliferation and migration, whereas miR‐155 inhibitor showed opposite effects. In addition, the tumor‐suppressing function of TGFβR2 was verified by using siRNA and TGFβR2 overexpressing plasmids. The results showed that miR‐155 promotes cell growth and migration by negatively regulating TGFβR2. Thus, miR‐155‐regulated TGFβR2 as a potential therapeutic target in GC.

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Section: Aberrantmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

Zhang

Sun

et al. 2018

Cancer Science

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“…In this study, we focused on the role of miR-10a, a downregulated miRNA in PCa, in regulating KDM4A and PCa progression. 20,21 MicroRNA profiling of 499 PCa tumor tissues and 52 normal tissues identified miR-10a as the most significant downregulated miRNA in PCa tumor F I G U R E 3 MiR-10a negatively regulated KDM4A in PCa cells. Hypermethylation of CpG islands of miR-10a led to decrease of miR-10a expression and was observed in gastric cancer patients.…”

Section: Discussionmentioning

confidence: 99%

MiR‐10a functions as a tumor suppressor in prostate cancer via targeting KDM4A

Xiang

et al. 2018

J of Cellular Biochemistry

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Deregulation of microRNAs contributes to the abnormal cell growth which is frequently observed in cancer. In the current study, we detected the expression and regulatory relationship between miR‐10a and Lysine‐specific demethylase 4A (KDM4A) to reveal their function in prostate cancer (PCa) progression. We found that miR‐10a levels were significantly decreased in PCa cell lines in comparison with the normal epithelial cell line RWPE‐1. Downregulation of miR‐10a levels was also observed in tumor tissues from PCa patients compared with the adjacent normal tissues. Enhanced expression of miR‐10a inhibited cell proliferation and colony forming capability of PCa cells. In addition, quantitative real‐time polymerase chain reaction and Western blot analysis showed a significant decrease of KDM4A in response to miR‐10a elevation in PCa cells. Using dual luciferase assay, we confirmed that KDM4A was a target gene for miR‐10a. Furthermore, Western blot analysis indicated that miR‐10a overexpression inactivated YAP signaling and suppressed transcription of YAP target genes. Additionally, cell growth arrest and colony forming capacity inhibition induced by miR‐10a overexpression could be reversed by YAP overexpression in PCa cells. More importantly, miR‐10a mimics inhibited PC‐3 tumor growth in nude mice accompanied with a remarkable reduction of KDM4A and YAP expression. In conclusion, our results uncovered a tumor suppressor role of miR‐10a in PCa via negative regulation of KDM4A and its downstream Hippo‐YAP pathway.

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“…Assays to quantify mature miRNAs were performed using TaqMan miRNA probes (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions. Quantitative RT-PCR was performed to quantify miRNA levels and mRNA levels (see our previous study for details [15]). The primers for PCR amplifications were as follows: KRAS (sense) 5'-GACTCTGAAGATGTACCTATGGTCCTA-3'; KRAS (antisense) 5'-CATCATCAACACCCTGTCTTGTC-3';GAPDH (sense) 5'-GATATTGTTGCCATCAATGAC-3';GAPDH (antisense) 5'-TTGATTTTGGAGGGATCTCG-3'.…”

Section: Methodsmentioning

confidence: 99%

MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS

Fan

Zhang

et al. 2017

Cell Physiol Biochem

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Background/Aims: MicroRNAs (miRNAs) have emerged as major regulators of tumour development and progression in non-small cell lung cancer (NSCLC). However, the role of miR-193a-3p in NSCLC is still unclear. Methods: Quantitative RT-PCR was used to detect miR-193a-3p expression levels in NSCLC tumour tissues. CCK8, EdU and cell migration assays were performed to analyse the biological functions of miR-193a-3p in NSCLC cells. Luciferase reporter assays were used to validate the bioinformatics-predicted target genes of miR-193a-3p. Western blotting and RNA/DNA interference carried out to evaluate the association between miR-193a-3p and KRAS. Results: miR-193a-3p expression was decreased in the NSCLC tumour tissues. We investigated the biological effects of miR-193a-3p both in vivo and in vitro and found that enforced expression of miR-193a-3p inhibited tumour formation and suppressed cell proliferation and cell migration. KRAS was found to be a potential target of miR-193a-3p, and dual luciferase reporter assays showed that miR-193a-3p directly binds to the 3’-untranslated region (3’-UTR) of KRAS mRNA. In addition, we found that changing the expression of KRAS had the opposite results to those induced by miR-193a-3p in the NSCLC cells. Importantly, simultaneous overexpression of miR-193a-3p and KRAS could counteract the effects of both on cellular functions. Conclusion: These findings highlight an important role for miR-193a-3p as a tumour suppressor in NSCLC pathogenesis via the regulation of KRAS expression.

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miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphoma

Cited by 44 publications

References 45 publications

MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

MiR‐10a functions as a tumor suppressor in prostate cancer via targeting KDM4A

MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS

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