miR‐122‐5p modulates the radiosensitivity of cervical cancer cells by regulating <i>cell division cycle 25A</i> (<i>CDC25A</i>)

Ding, Feng-Na; Gao, Bao-Hong; Wu, Xia; Gong, Chun-Wu; Wang, Weiqing; Zhang, Shumao

doi:10.1002/2211-5463.12730

Cited by 32 publications

(27 citation statements)

References 41 publications

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“…After achieving the transport and encapsulation of exogenously added miRNA into exosomes and EV secretion in resealed HeLa cells, we examined the effect of anti-Ago and anti-YBX1 antibodies on the transport of miRNA mimics to CD63-positive MVEs. We selected these miRNAs (miR-122 40 – 43 , miR-34a 40 , 44 , 45 , and miR-423-3p 40 , 46 , 47 ) because they are expressed in HeLa cells 40 , 41 , 44 , 46 . Furthermore, they interact with Ago proteins 16 , 42 , 43 , 45 , 47 , and studies have investigated their sorting mechanism 16 , 18 , 46 .…”

Section: Resultsmentioning

confidence: 99%

Applications of cell resealing to reconstitute microRNA loading to extracellular vesicles

Sonoda

Kano

Murata

2021

Sci Rep

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MicroRNAs (miRNAs) are cargo carried by extracellular vesicles (EVs) and are associated with cell–cell interactions. The response to the cellular environment, such as disease states, genetic/metabolic changes, or differences in cell type, highly regulates cargo sorting to EVs. However, morphological features during EV formation and secretion involving miRNA loading are unknown. This study developed a new method of EV loading using cell resealing and reconstituted the elementary miRNA-loading processes. Morphology, secretory response, and cellular uptake ability of EVs obtained from intact and resealed HeLa cells were comparable. Exogenously added soluble factors were introduced into multivesicular endosomes (MVEs) and their subsequent secretion to the extracellular region occurred in resealed HeLa cells. In addition, miRNA transport to MVEs and miRNA encapsulation to EVs followed a distinct pathway regulated by RNA-binding proteins, such as Argonaute and Y-box binding protein 1, depending on miRNA types. Our cell-resealing system can analyze disease-specific EVs derived from disease model cells, where pathological cytosol is introduced into cells. Thus, EV formation in resealed cells can be used not only to create a reconstitution system to give mechanistic insight into EV encapsulation but also for applications such as loading various molecules into EVs and identifying disease-specific EV markers.

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Section: Resultsmentioning

confidence: 99%

Applications of cell resealing to reconstitute microRNA loading to extracellular vesicles

Sonoda

Kano

Murata

2021

Sci Rep

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“…miR-125a promotes sensitivity to paclitaxel, while upregulated miR-375 is associated with increased resistance to this chemotherapeutic [145,146]. Downregulation of miR-122-5p, miR-375, and miR-449b and upregulation of miR-21 and miR-125 are related to radioresistance of CC cells [147][148][149][150][151]. Determination of biomarkers contributing to metastasis ability and susceptibility to standard treatment may significantly increase the positive outcomes of the disease by individualized choice of effective therapeutic method.…”

Section: Prognostic Value-susceptibility To Conventional Therapymentioning

confidence: 99%

MicroRNA-Based Fingerprinting of Cervical Lesions and Cancer

Pisarska

Baldy-Chudzik

2020

JCM

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The regulatory functions of microRNA (miRNA) are involved in all processes contributing to carcinogenesis and response to viral infections. Cervical cancer in most cases is caused by the persistence of high-risk human papillomavirus (HR-HPV) infection. While oncogenic human papillomaviruses induce aberrant expression of many cellular miRNAs, this dysregulation could be harnessed as a marker in early diagnosis of HR-HPV infection, cervical squamous intraepithelial lesions, and cancer. In recent years, growing data indicate that miRNAs show specific patterns at various stages of cervical pathology. The aim of this review is to systematize current reports on miRNA capacity that can be utilized in personalized diagnostics of cervical precancerous and cancerous lesions. The analysis of the resources available in online databases (National Center for Biotechnology Information—NCBI, PubMed, ScienceDirect, Scopus) was performed. To date, no standardized diagnostic algorithm using the miRNA pattern in cervical pathology has been defined. However, the high sensitivity and specificity of the reported assays gives hope for the development of non-invasive diagnostic tests that take into account the heterogeneity of tumor-related changes. Due to this variability resulting in difficult to predict clinical outcomes, precise molecular tools are needed to improve the diagnostic and therapeutic process.

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“…miRNAs push forward an immense influence on a series of biological processes which are closely related to cancers, such as differentiation, angiogenesis, proliferation and stress response [ 12 ]. As to miR-122-5p, an update report has mentioned its regulatory function in the radiosensitivity of CC cells [ 13 ]. Moreover, another study has also presented that lncRNA MIR205HG promotes the growth and development of CC as a competing endogenous RNA (ceRNA) through sponging miR-122-5p [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Exosomal lncRNA UCA1 modulates cervical cancer stem cell self-renewal and differentiation through microRNA-122-5p/SOX2 axis

Gao

Wang

et al. 2021

J Transl Med

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Background There is growing evidence discussing the role of long non-coding RNAs (lncRNAs) in cervical cancer (CC). We performed this study to explore the impact of exosomal lncRNA urothelial cancer-associated 1 (UCA1) in CC stem cells by sponging microRNA-122-5p (miR-122-5p) and regulating SOX2 expression. Methods CC stem cells (CD133+CaSki) and exosomes were extracted and identified. The synthesized UCA1- and miR-122-5p-related sequences were transfected into CaSki cells, CaSki cells-derived exosomes were extracted and then co-cultured with CD133+CaSki cells. The functional roles of UCA1 and miR-122-5p in self-renewal and differentiation ability of CC stem cells were determined using ectopic expression, knockdown/depletion and reporter assay experiments. An in vivo experiment was performed to verify the in vitro results. Results Up-regulated UCA1 and SOX2 and down-regulated miR-122-5p were found in CaSki-Exo. Exosomes promoted invasion, migration, proliferation and restrained apoptosis of CD133+CaSki cells. Silencing UCA1 or up-regulating miR-122-5p degraded SOX2 expression, and reduced invasion, migration and proliferation of CD133+CaSki cells while advanced apoptosis and suppressed the tumor volume and weight in nude mice. Conclusion Our study provides evidence that CaSki-Exo can promote the self-renewal and differentiation ability of CC stem cells while silencing UCA1 or up-regulating miR-122-5p restrains self-renewal and differentiation of CC stem cells.

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miR‐122‐5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A)

Cited by 32 publications

References 41 publications

Applications of cell resealing to reconstitute microRNA loading to extracellular vesicles

Applications of cell resealing to reconstitute microRNA loading to extracellular vesicles

MicroRNA-Based Fingerprinting of Cervical Lesions and Cancer

Exosomal lncRNA UCA1 modulates cervical cancer stem cell self-renewal and differentiation through microRNA-122-5p/SOX2 axis

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