MiR-1224-5p acts as a tumor suppressor by targeting CREB1 in malignant gliomas

Qian, Jin; Li, Rui; Wang, Yingyi; Shi, Yan; Luan, Wenkang; Tao, Tao; Zhang, Junxia; Xu, Yichang; You, Yongping

doi:10.1007/s11010-015-2334-1

Cited by 50 publications

(45 citation statements)

References 40 publications

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“…The mechanism by which CREB regulates glioblastoma tumor cell proliferation involves activities downstream from both the MAPK and PI3K pathways that then modulate the expression of three key cell cycle factors, cyclin B1, cyclin D1 and PCNA [32]. It has been shown that miR-1224-5p and miR-200b act as tumor suppressors by targeting CREB in malignant gliomas [33, 34]. Herein, we demonstrated that CREB is a direct target of miR-433-3p in glioma cells.…”

Section: Discussionmentioning

confidence: 99%

MiR-433-3p suppresses cell growth and enhances chemosensitivity by targeting CREB in human glioma

et al. 2016

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Previous studies reported that miR-433 exerts function widely in human tumorigenesis and development. Here, we further investigate the potential role of miR-433 in glioma. Quantitative real-time PCR demonstrated that miR-433-3p and miR-433-5p were low expressed in glioma tissues and cell lines. Functional studies suggested that the overexpression of miR-433-3p suppressed proliferation, induced apoptosis and inhibited invasion and migration of human glioma cells. But the growth and metastasis of glioma cells were not significantly influenced by overexpression of miR-433-5p. In a xenograft model, we also showed that miR-433-3p had an inhibitory effect on the growth of glioma. Bioinformatics coupled with luciferase and western blot assays revealed that CREB is a direct target of miR-433-3p, and the overexpression of CREB can rescue the phenotype changes induced by miR-433-3p overexpression. Besides, miR-433-3p could increase chemosensitivity of glioma to temozolomide by targeting CREB in vitro and in vivo. Taken together, these results suggest that miR-433-3p may function as a potential marker in diagnostic and therapeutic target for glioma.

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Section: Discussionmentioning

confidence: 99%

MiR-433-3p suppresses cell growth and enhances chemosensitivity by targeting CREB in human glioma

et al. 2016

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“…Nymark et al reported that miR-1224-5p is remarkably decreased in asbestos-related lung cancer (16). In addition, miR-1224-5p acts as a tumor suppressor to repress cell proliferation and invasion and promote apoptosis by downregulating the expression of CREB1 in malignant gliomas (17). Furthermore, the up-regulation of miR-1224-5p inhibits the proliferation and invasion and promotes the apoptosis of keloid cells via the TGF-β1/Smad3 signaling pathway (18).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-1224-5p Inhibits Metastasis and Epithelial-Mesenchymal Transition in Colorectal Cancer by Targeting SP1-Mediated NF-κB Signaling Pathways

Peng

Yang

et al. 2020

Front. Oncol.

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MicroRNAs (miRNAs) are small non-coding RNAs that play pivotal roles in cancer initiation and progression. However, the roles and molecular mechanisms of miRNAs in colorectal cancer (CRC) progression remain unclear. Here, we show that downregulation of miR-1224-5p in CRC is negatively correlated with SP1 expression and metastasis in patients and xenografted mouse models. Gain-and loss-of-function assays reveal that miR-1224-5p suppresses the migration, invasion, and epithelial-mesenchymal transition (EMT) of CRC cells in vitro and in vivo by directly targeting SP1. Moreover, SP1 promotes the phosphorylation of p65, which results in EMT progress in CRC cells. Clinical analysis reveals that miR-1224-5p and SP1 expression are remarkably associated with advanced clinical features and unfavorable prognosis of patients with CRC. Further study confirms that hypoxia accounts for the depletion of miR-1224-5p in CRC. The enhancement of hypoxia during epithelial-mesenchymal transition and metastasis of CRC cells is abolished by miR-1224-5p. Our findings provide the first evidence that miR-1224-5p is a potential therapeutic target and prognostic biomarker for patients with CRC.

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“…A total of 10 miRNAs were negatively correlated with TACC3 expression and qualified as having a binding site within the TACC3 mRNA 3′ untraslational region. Some of these miRNAs, such as miR-1224-5p [27], miR-30e-3p [28], miR-488-3p [29], miR-491-5p [30], miR-940 [31] and miR-885-3p [32], are proven as tumor suppressors for various cancers. In the CGGA miRNA dataset, these TACC3 negatively associated miRNAs were significantly downregulated in high grade glioma (except for miR-330-3p, p = 0.4164) (Figure S5).…”

Section: Resultsmentioning

confidence: 99%

Overexpression of Transforming Acidic Coiled Coil‑Containing Protein 3 Reflects Malignant Characteristics and Poor Prognosis of Glioma

Sun

Tian

Wang

et al. 2017

IJMS

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Gliomas are malignant primary brain tumors with poor prognosis. Recently, research was indicative of a tight connection between tumor malignancy and genetic alterations. Here, we propose an oncogenic implication of transforming acidic coiled-coil-containing protein 3 (TACC3) in gliomas. By comprehensively analyzing the Chinese glioma genome atlas (CGGA) and publicly available data, we demonstrated that TACC3 were overexpressed along with glioma grade and served as an independent negative prognostic biomarker for glioma patients. Functions’ annotations and gene sets’ enrichment analysis suggested that TACC3 may participate in cell cycle, DNA repair, epithelium-mesenchymal transition and other tumor-related biological processes and molecular pathways. Patients with high TACC3 expression showed CD133+ stem cell properties, glioma plasticity and shorter overall survival time under chemo-/radio-therapy. Additionally, a TACC3 associated the miRNA-mRNA network was constructed based on in silico prediction and expression pattern, which provide a foundation for further detection of TACC3-miRNA-mRNA axis function. Collectively, our observations identify TACC3 as an oncogene of tumor malignancy, as well as a prognostic and motoring biomarker for glioma patients.

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MiR-1224-5p acts as a tumor suppressor by targeting CREB1 in malignant gliomas

Cited by 50 publications

References 40 publications

MiR-433-3p suppresses cell growth and enhances chemosensitivity by targeting CREB in human glioma

MiR-433-3p suppresses cell growth and enhances chemosensitivity by targeting CREB in human glioma

MicroRNA-1224-5p Inhibits Metastasis and Epithelial-Mesenchymal Transition in Colorectal Cancer by Targeting SP1-Mediated NF-κB Signaling Pathways

Overexpression of Transforming Acidic Coiled Coil‑Containing Protein 3 Reflects Malignant Characteristics and Poor Prognosis of Glioma

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