miR-126 inhibits non-small cell lung cancer cells proliferation by targeting EGFL7

Sun, Yanqin; Bai, Yifeng; Zhang, Fan; Wang, Yu; Guo, Ying; Guo, Linlang

doi:10.1016/j.bbrc.2009.12.098

Cited by 160 publications

(127 citation statements)

References 36 publications

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“…137 Such miRNAs as miR-126, miR-31, miR-519c, Let-7a, miR-133B, miR-15a, miR-16, and miR-183 have been found to regulate lung cancer cell proliferation, migration and invasion by targeting specific molecules, including Crk, EGFL7, VEGF, LATS2, PPP2R2A, HIF-1a, NIRF, MCL-1, BCL2L2, cyclins D1, D2 and E1, and Ezrin. [138][139][140][141][142][143][144][145][146] Abnormally expressed miRNAs may have potential as markers in lung cancer diagnosis, treatment response, and prognosis. Has-miR-205, which suppresses epithelial-to-mesenchymal transition, has been identified as a highly specific marker for squamous carcinoma of the lung.…”

Section: Histone Modificationmentioning

confidence: 99%

Genetic and epigenetic changes in lung carcinoma and their clinical implications

Wen

Zhang

et al. 2011

Modern Pathology

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Lung cancer is the leading cause of cancer deaths worldwide. Recent advance in targeted therapy for lung cancer patients with epidermal growth factor receptor (EGFR) mutations has demonstrated a promising development toward personalized therapy for lung cancer patients. The development of lung cancer is a complex process, involving a series of genetic and epigenetic changes. Tobacco smoke is the predominant etiologic risk factor for lung cancer. However, some lung cancers, especially adenocarcinomas, arise in patients who have never smoked, suggesting the importance of host genetic/epigenetic susceptibility in the occurrence and development of lung cancer. Understanding of these genetic and epigenetic changes will further aid in the biomarker-driven personalized therapy for lung cancer patients. In this review, we summarize the genetic and epigenetic alterations observed in lung cancers, including chromosomal loss of heterozygosity, tumor-suppressor gene mutation, gene methylation, histone modification, and microRNA expression changes. Clinical and preclinical studies have implied specific genetic/epigenetic changes for clinical application in lung cancer patients. However, more efforts are required in validation of the identified molecular markers in lung cancer patients for early detections, assessment for treatment response, and survival predictions.

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Section: Histone Modificationmentioning

confidence: 99%

Genetic and epigenetic changes in lung carcinoma and their clinical implications

Wen

Zhang

et al. 2011

Modern Pathology

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“…miRNAs that are upregulated in cancer may function as oncogenes through the negative regulation of tumor suppressor genes, whilst miRNAs that are downregulated may function as tumor suppressor genes and inhibit cancer by regulating oncogenes (30,31). miR-126 acts as a metastatic suppressor in a number of human cancers (21,23). However, the expression and function of miR-126 in ovarian cancer remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…miR-126 originates from a common precursor structure located within the EGFL7 gene, and its expression levels have been reported to vary in a number of human cancers; patients with low miR-126 expression exhibit poor survival compared with patients with high miR-126 levels (20)(21)(22)(23). It has been proposed that miR-126 is essential in the inhibition of the invasive growth of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

microRNA-126 suppresses PAK4 expression in ovarian cancer SKOV3 cells

et al. 2015

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Abstract. Primary ovarian cancer is one of the predominant causes of mortality from gynecological cancer. The suppression of serine/threonine p21-activated kinases (PAKs), proteins involved in cell morphology and cytoskeletal reorganization, has been hypothesized to improve the survival of patients with ovarian cancer. However, the association between microRNA-126 (miR-126) and PAK4 in the inhibition of ovarian cancer cell invasion remains to be established. The present study demonstrated changes in the level of PAK4 expression in ovarian cancer SKOV3 cells with altered miR-126 compared with normal SKOV3 cells. The SKOV3 cells that were transfected with LV3-miR-126 to increase miR-126 expression exhibited significantly downregulated expression levels of PAK4 (P<0.05), whilst transfection with the LV3-hsa-miR-126 inhibitor increased the expression of PAK4 in these cells (P<0.05), as assessed by immunofluorescence staining. Furthermore, western blot analysis revealed a significant increase in PAK4 expression in the SKOV3 cells transfected with the LV3-hsa-miR-126 inhibitor, and a decrease in those transfected with LV3-hsa-miR-126. The present study provides an experimental foundation for miR-126 as a potential tumor suppressor that may decrease PAK4 expression to inhibit ovarian cancer cells.

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“…The epidermal growth factor receptor (EGFR) encoded by the target of hsa-miR-145 may inhibit cancer growth (28); the downregulation of hsa-miR-145 in SCLC may generate an increase in the expression levels of EGFR and thus promote cancer growth. The targets of hsa-miR-126 encode epidermal growth factor-like domain 7 (EGFL7), which is involved in cellular responses, such as cell migration and blood vessel formation (29). The downregulation of hsa-miR-126 may activate EGFL7 and advance tumor growth in vivo.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and expression analysis of microRNA involved in small cell lung cancer via deep sequencing

Yan

Shi

Wang

et al. 2014

Molecular Medicine Reports

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Abstract. Small cell lung cancer is a major cause of mortality worldwide. microRNAs (miRNAs) are involved in various biological processes through regulating gene expression. In the present study, to identify the miRNAs involved in human small cell lung cancer at the genome-wide level, Solexa sequencing was employed to sequence two small RNA (sRNA) libraries from small cell lung cancer tissues (LC sRNA library) and the corresponding normal tissues (NT sRNA library). Deep sequencing of the two sRNA libraries identified a number of conserved miRNAs and differential expression analysis of these miRNAs revealed 81 miRNAs differentially expressed in small cell lung cancer, of which more than half were downregulated. The expression trends determined by sequencing were validated by reverse transcription-quantitative polymerase chain reaction analysis. The annotations for the targets of these miRNAs were predicted. This study provides valuable information for understanding the regulatory mechanisms of miRNAs involved in human small cell lung cancer. IntroductionMicroRNAs (miRNAs) are small non-coding RNA molecules, ~22 nucleotides long, which are widely distributed in plants and animals (1,2). The primary miRNA transcripts are cleaved by Drosha and Dicer enzymes to form mature miRNAs, which serve as posttranscriptional negative gene regulators by either cleaving mRNA or inhibiting translation (3,4). Through such mechanisms, miRNAs are involved in various biological processes, including organ development, tissue differentiation, cell cycle regulation and cancer development (4-6).Lung cancer is the predominant cause of cancer-related mortality worldwide and its pathogenesis is closely associated with tobacco smoking. Lung cancer is categorized into two main histological groups: Non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). Regulation of miRNAs is closely associated with tumor initiation, promotion and progression (7). Recently, increasing evidence has demonstrated that miRNAs may be associated with lung cancer. A previous study revealed that an increase in hsa-miR-196a expression levels was a characteristic molecular change in NSCLC (8,9), and that hsa-miRNA-196a promoted NSCLC cell proliferation and invasion via targeting HOXA5 (10). Overexpression of miR-200 was shown to reduce the expression levels of NSCLC prognostic biomarkers in H1299 and BEAS-2B cells (11). miR-449a, which inhibited migration and invasion through targeting c-Met, was found to be downregulated in NSCLC tissues and cell lines (12). Reduced expression of miR-101 was associated with overexpression of EZH2 and exhibited tumor-suppressive functions in NSCLC (13). This evidence suggests that miRNAs are crucial in NSCLC; however, little is known regarding the functions and regulation of miRNAs in SCLC.In the present study, Solexa sequencing was used to generate a large quantity of small RNA (sRNA) data for SCLC and corresponding normal tissues. Sequence analysis was employed to identify specific miRNAs associated with SCLC and these ...

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miR-126 inhibits non-small cell lung cancer cells proliferation by targeting EGFL7

Cited by 160 publications

References 36 publications

Genetic and epigenetic changes in lung carcinoma and their clinical implications

Genetic and epigenetic changes in lung carcinoma and their clinical implications

microRNA-126 suppresses PAK4 expression in ovarian cancer SKOV3 cells

Genome-wide identification and expression analysis of microRNA involved in small cell lung cancer via deep sequencing

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