miR-1267 Induces Tumorigenicity and Contributes to Risk of Clear Cell Renal Cell Carcinoma

Abstract

Objective: Dysregulated microRNA signatures in different cancer types are being uncovered continually implying their significance in cancer pathogenesis. miR-1267 was not previously associated with RCC. In this study, it is aimed to obtain the expression profile of miR-1267 in patients with ccRCC and its correlation with patient parameters. Methods: Kidney Cancer cDNA Array consisting of cDNA samples obtained from healthy kidney tissues of 4 healthy individuals and tumoral kidney tissues of 5 Stage I, 5 Stage II, 3 Stage III and 2 Stage IV ccRCC patients was used. Hsa-miR-1267 and SNORD48 (as housekeeping gene) expressions were analyzed. miR-1267 expression was statistically correlated with the clinical parameters of patients. miRGator 3.0 database was used to compare miR-1267 expression patterns of different urological cancer types. Results: The expression of miR-1267 was significantly higher in male than female (p=0.027). Also, there were statistically significant increase in miR-1267 expression in stage IV when compared to stage I (p<0.001). Moreover, increased platelet/lymphocyte ratio and calcium level, which were parameters giving information about the occurrence of ccRCC, are significantly associated with increased miR-1267 expression (p<0.001 and p=0.003, respectively). The expression of miR-1267 in kidney tumor tissues was higher approximately three times than normal kidney tissues (p>0.05). Conclusion: miR-1267 could have oncogenic function, have predictive value for RCC development and be predictive about aggressiveness in ccRCC.