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In study, it was aimed to determine the stress effects that can be caused by transporting and altitude in sheep. Karayaka sheeps were used in the study. Karayaka sheeps are a valuable breed of meat quality and fleece, which is raised in the Black Sea region. The live weight of the sheep (n = 30) while hungry was determined before transport and sea level. Average live weight was determined as 55.64 ± 4.66 kg. Blood samples were collected just before (sea level) and just after transport (1500 meters above sea level). Transportation distance was approximately 182 km and duration was 5 hours. According to the findings, cortisol was not affected by transport stress and altitude (P>0.05) and Triiyodotironin (T3) (P<0.039) and Tyrosine (T4) (P<0.000) were affected significantly. Malondialchehyche (MDA), which is one of the oxidative stress parameters, was significantly affected (P<0.039) and Protein Carbonyl (PC) values were not affected by transport and altitude (P>0.184). As a result of this study, it was determined that transportation and altitude in sheep causes stress. Stress-reducing measures should be taken in the exposure of sheep to altitude differences and in transportation. Antioxidant nutritional supplements should be made in order not to negatively affect the meat quality in sheep.

Özet: Bu çalışmada, yavaş gelişen iki farklı etlik piliç hattında yumurta içi (in ovo) propolis enjeksiyonunun ve enjeksiyon yerinin kuluçka randımanı, çıkış ağırlığı ve yaşama gücü üzerine etkilerini değerlendirmek amacı ile iki deneme yürütülmüştür. Deneme 1'de Ross x (Ross x Rhode Island Red) melezi etlik piliç anaçlarından (30 haftalık yaşta) elde edilen 70 adet döllü yumurta, 2 (tuz ve propolis solusyonu) × 2 (hava kesesi ve amniyotik sıvı) faktöriyel dizaynda 4 grubuna dağıtılmıştır. Deneme 2'de Ross x Barred Plymouth Rock melezi etlik piliç anaçlarından (32 haftalık yaşta) elde edilen 64 adet döllü yumurta 3 gruba tesadüfî olarak dağıtılmıştır. Deneme 1'de inkübasyonun 19. gününde 0.5 ml % 0.9 tuz veya % 4.6 propolis solüsyonları, yumurtanın hava kesesi veya amniyotik sıvısına, Deneme 2'de ise inkübasyonun 18. gününde 0.5 ml % 0.9 tuz solüsyonu yumurtanın hava kesesi veya amniyotik sıvısına küt ve sivri ucundan enjekte edilmiştir. Her iki denemede de in ovo propolis enjeksiyonu ve enjeksiyon yeri kuluçka randımanı, çıkış ağırlığı ve yaşama gücünü etkilememiştir. Bununla birlikte, 1. denemede kuluçka randımanı ve çıkış ağırlığı in ovo enjeksiyon bölgesine sırasıyla bağımlı (χ 2 = 8.020, P<0.01) ve bağımlı olma eğiliminde (P<0.10) olmuştur. Sonuçta, hava kesesine in ovo propolis uygulamasının çıkış randımanını arttırmasına rağmen, civciv ağırlığını düşürme eğiliminde olduğu söylenebilir.Anahtar Kelimeler: Kümes hayvanları, propolis, yumurta içi besleme, enjeksiyon bölgesi, civciv çıkış özellikleri The Effects of In Ovo Injection of Propolis and Injection Site on Hatchability, Hatching Weight and Survival of ChicksAbstract: In this study, two experiments were conducted to evaluate the effects of in ovo injection of propolis and injection sites in ovo on hatchability, hatching weight and survival of newly-hatched chicks in two different lines of slow-growing broiler. In Experiment 1, 70 fertile eggs from Ross x Rhode Island Red breeder (30-week-old) were allocated to four groups according to a 2 (saline and propolis solution) × 2 (air cell and amniotic fluid) factorial arrangement. In Experiment 2, 64 fertile eggs from Ross x Barred Plymouth Rock breeder (32-week-old) were allocated randomly to three groups. In Experiment 1, a 0.5 ml of 0.9% saline or 4.6% propolis solution was administered to the air cell or the amniotic fluid of eggs at day 19 of incubation. In Experiment 2, at 18 d of incubation 0.5 ml of 0.9% saline solution was administered to the air cell or the amnion at the blunt end and the pointed end of the egg. In the both experiments, the hatchability, hatching weight and survival of newly-hatched chicks were not affected by in ovo injection of propolis and injection sites. However, in Experiment 1, while the hatchability (χ 2 = 8.020, P<0.01) was dependent on the injection sites, hatching weight of newlyhatched chicks (P < 0.10) was tend to be dependent on the injection sites. In conclusion, it can said that in ovo administration of propolis to the air cell increased the hatchability, but was te...

Objective: Dysregulated microRNA signatures in different cancer types are being uncovered continually implying their significance in cancer pathogenesis. miR-1267 was not previously associated with RCC. In this study, it is aimed to obtain the expression profile of miR-1267 in patients with ccRCC and its correlation with patient parameters. Methods: Kidney Cancer cDNA Array consisting of cDNA samples obtained from healthy kidney tissues of 4 healthy individuals and tumoral kidney tissues of 5 Stage I, 5 Stage II, 3 Stage III and 2 Stage IV ccRCC patients was used. Hsa-miR-1267 and SNORD48 (as housekeeping gene) expressions were analyzed. miR-1267 expression was statistically correlated with the clinical parameters of patients. miRGator 3.0 database was used to compare miR-1267 expression patterns of different urological cancer types. Results: The expression of miR-1267 was significantly higher in male than female (p=0.027). Also, there were statistically significant increase in miR-1267 expression in stage IV when compared to stage I (p<0.001). Moreover, increased platelet/lymphocyte ratio and calcium level, which were parameters giving information about the occurrence of ccRCC, are significantly associated with increased miR-1267 expression (p<0.001 and p=0.003, respectively). The expression of miR-1267 in kidney tumor tissues was higher approximately three times than normal kidney tissues (p>0.05). Conclusion: miR-1267 could have oncogenic function, have predictive value for RCC development and be predictive about aggressiveness in ccRCC.

The aim of this study was to determine the macroscopic and microscopic spermatological parameters of the Karayaka rams grown in public hands. Out off-season sperm couldn’t be taken from the rams. During the season, semen was collected from all 2-4 age of Karayaka rams (n = 10). Weight (kg) and circumference of the scrotum (cm) were also recorded. When the collected spermatozoa were examined, it was found that the weight variable correlated only with the pH variable. The correlation coefficient calculated between weight and pH was -0,802 and it was found to be statistically significant. The correlation coefficient, which calculated as 0.680, between the circumference of the scrotum (cm) and mortality (% Dead-Live) shows that one feature increases while the other increases significantly. Viscosity (1-5) was correlated only with mass activity (Mass Activity 0-4), and the degree of this relationship was calculated as -0.655. Variables were not associated with sperm volume (ml), motility (%) and density (109).