Mir‐1287 suppresses the proliferation, invasion, and migration in hepatocellular carcinoma by targeting PIK3R3

Lu, Ji; Tang, Licheng; Xu, Yuqiang; Ge, Kuikui; Huang, Jinjiang; Gu, Meigang; Zhong, Jun; Huang, Qingshan

doi:10.1002/jcb.27190

Cited by 25 publications

(13 citation statements)

References 33 publications

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“…Previous studies revealed that miR-1287-5p serves an important role in breast (19), colorectal (20) and cervical (21) cancers. A previous study indicated that miR-1287-5p inhibits HCC progression by targeting PIK3R3 (22). The present study found that knockdown of circ-CCT3 enhanced miR-1287-5p expression and that downregulation of miR-1287-5p resulted in a reversal effect of sh-circ-CCT3-1 in HCC cells.…”

Section: Discussionsupporting

confidence: 64%

Circular RNA circ‑CCT3 promotes hepatocellular carcinoma progression by regulating the miR‑1287‑5p/TEAD1/PTCH1/LOX axis

et al. 2021

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Hepatocellular carcinoma (HCC) is characterized by a poor prognosis because of its insensitivity to radiation and chemotherapy. Recently, circular RNAs (circRNAs) have been found to serve important roles in hepatocellular carcinogenesis. circ-CCT3, a novel circRNA, was screened from the differential tissue expression results of a circRNA microarray. Relative expression levels of circ-CCT3 in specimens and cell lines were evaluated by reverse transcription-quantitative PCR and the relationship between circ-CCT3 and prognosis was analyzed by Kaplan-Meier curves. The oncogenic role of circ-CCT3 was confirmed in HCC cells through a cell counting kit-8 (CCK-8) assay, a colony formation assay, acridine orange/ethidium bromide double fluorescence staining, flow cytometry, a wound-healing assay and a Transwell assay. Bioinformatics prediction and luciferase reporter assays validated that circ-CCT3 facilitated HCC progression through the miR-1287-5p/TEA domain transcription factor 1 (TEAD1) axis. TEAD1 could then directly activate patched 1 and lysyl oxidase transcription, as analyzed by chromatin immunoprecipitation and luciferase reporter assays. The present study identified a novel circRNA, circ-CCT3, which may be used as a potential therapeutic target for HCC.

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Section: Discussionsupporting

confidence: 64%

Circular RNA circ‑CCT3 promotes hepatocellular carcinoma progression by regulating the miR‑1287‑5p/TEAD1/PTCH1/LOX axis

et al. 2021

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“…29 Lu et al declare that upregulation of miR-1287 decreases the tumorigenesis phenotypes of HCC cells in vitro, including cell proliferation, cell cycle, invasion and migration in vitro model. 14 In this project, the downregulation of miR-1287 is detected in HCC tissues and cells. Next, we find that depletion of miR-1287 reverses the circ_CDR1as depletion-mediated the reduced tumorigenesis phenotypes of HCC cells.…”

Section: Discussionmentioning

confidence: 98%

“…13 MiRNA-1287 (miR-1287) has been documented to play as a tumor suppressor in HCC. 14 Having known that miR-1287 is a potential target of circ_CDR1as (predicted utilizing starBase), we intend to explore the impact of miR-1287 on the circ_CDR1as-mediated HCC progression, so as to better understand the role of circ_CDR1as in HCC.…”

Section: Introductionmentioning

confidence: 99%

<p>CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway</p>

Zhang¹,

Li²,

Zhu³

et al. 2020

CMAR

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Background: Hepatocellular carcinoma (HCC) is a common lethal malignant tumor worldwide. Circular RNAs (circRNAs) have been reported to affect the development of human cancers, including HCC. In this project, we aim to clarify the functional effect of circular CDR1as (circ_CDR1as) on HCC progression. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot is implemented to detect the expression of circ_CDR1as, microRNA (miR)-1287 and Raf-1 proto-oncogene, serine/threonine kinase (Raf1). Cell proliferation is assessed via colony formation and 3-(4, 5)-dimethylthiazole-2-y1)-2, 5-biphenyl tetrazolium bromide (MTT) assays. Cell migration and invasion are measured by Transwell assay. The target relationship between miR-1287 and circ_CDR1as or Raf1 is validated through dual-luciferase reporter assay. The levels of epithelia-mesenchymal transition (EMT) markers and the MEK/ERK signal pathway-related proteins are examined by Western blot. Model in nude mice is constructed to determine the role of circ_CDR1as in vivo. Results: Expression of circ_CDR1as and Raf1 is elevated, while miR-1287 expression is decreased in HCC. Depletion of circ_CDR1as or Raf1 could inhibit proliferation and metastasis of HCC cells. Besides, circ_CDR1as regulates Raf1 expression by targeting miR-1287. MiR-1287 upregulation or Raf1 depletion could partially counterbalance circ_CDR1as depletion-mediated inhibitory effects on HCC cell behaviors. Moreover, circ_CDR1as depletion represses HCC progression through inactivating MEK/ERK pathway. In addition, circ_CDR1as depletion suppresses tumor growth in vivo via regulating miR-1287/Raf1 pathway. Conclusion: Circ_CDR1as depletion inhibits HCC cell proliferation and metastasis by miR-1287/Raf1 and MEK/ERK pathways, highlighting a promising molecular target for HCC treatment.

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“…Moreover, Jones et al found that both SH2 groups in each of the three PI3KR isoforms bound with moderate or high affinity at multiple sites in ERBB3, including PIK3R3. PIK3R3 is associated with the activation of the class IA PI3K complex, as one of its regulatory subunits . PIK3R3 promotes tumor growth or metastasis in esophageal squamous cell carcinoma, glioma, HCC, ovarian cancer, gastric cancer, colorectal cancer and pancreatic cancer .…”

Section: Discussionmentioning

confidence: 99%

Epithelial V‐like antigen 1 promotes hepatocellular carcinoma growth and metastasis via the ERBB‐PI3K‐AKT pathway

Chen

Zheng

et al. 2020

Cancer Science

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The role of epithelial V-like antigen 1 (EVA1) has been well studied in thymic development and homostasis; however, its putative relationship with cancer remains largely unknown. Therefore, here we investigated the role of EVA1 in hepatocellular carcinoma. Interestingly, EVA1 expression was significantly increased in hepatocellular carcinoma (HCC) and was also associated with a poor prognosis and recurrence in HCC patients. Overexpression of EVA1 promoted cell growth, invasion and migration in vitro. Consistently, knockdown of EVA1 expression inhibited proliferation and migration in vitro, while repressing metastasis of HCC cells in vivo. RNA-seq analysis indicated that EVA1 is able to upregulate the expression of genes in the ERBB3-PI3K pathway. Accordingly, an increased level of AKT phosphorylation was detected in HCC cells after EVA1 overexpression. LY294002, a PI3K inhibitor, inhibited AKT phosphorylation and rescued the tumor-promoting effect of EVA1 overexpression. Altogether, the present study has revealed the oncogenic role of EVA1 during HCC progression and metastasis through the ERBB-PI3K-AKT signaling pathway, reiterating the potential use of EVA1 as a therapeutic target and/or prognostic marker for HCC. K E Y W O R D SAKT, EVA1, HCC, metastasis, PIK3R3 | INTRODUC TI ONHepatocellular carcinoma (HCC) is the fifth most common type of malignant tumor and a leading cause of cancer-related death worldwide. 1 HCC is a complex malignancy with various genetic abnormalities. Although HCC patients can be treated by surgical resection, radiotherapy and/or sorafenib, many HCC patients die due to recurrence, metastasis and blood vessel invasion. [2][3][4] Therefore, exploring the molecular mechanism of HCC tumorigenesis and metastasis may help to understand the pathogenesis of HCC and to find potential molecular targets for treatment of this malignancy.This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Mir‐1287 suppresses the proliferation, invasion, and migration in hepatocellular carcinoma by targeting PIK3R3

Cited by 25 publications

References 33 publications

Circular RNA circ‑CCT3 promotes hepatocellular carcinoma progression by regulating the miR‑1287‑5p/TEAD1/PTCH1/LOX axis

Circular RNA circ‑CCT3 promotes hepatocellular carcinoma progression by regulating the miR‑1287‑5p/TEAD1/PTCH1/LOX axis

<p>CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway</p>

Epithelial V‐like antigen 1 promotes hepatocellular carcinoma growth and metastasis via the ERBB‐PI3K‐AKT pathway

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