miR‑1301‑3p promotes the proliferation and migration of lung cancer cells via direct repression of polymerase I and transcript release factor

Abstract

Aberrant expression of microRNAs (miRNAs or miRs) is associated with a number of human diseases, including lung cancer. Although numerous differentially expressed miRNAs have been identified in lung cancer via microarray and sequencing methods, to the best of our knowledge, only a small portion of these miRNAs have been experimentally verified. In the present study, miR-1301-3p expression levels in lung tumor tissues and lung cancer cells were measured by reverse transcription-quantitative PCR (RT-qPCR) and by analyzing previously published data. Cell Counting Kit-8 and Transwell assays were used to analyze the function of miR-1301-3p in lung cancer tissues and cells. Bioinformatics analysis, RT-qPCR, western blotting and a dual-luciferase reporter assay were performed to investigate the mechanism of miR-1301-3p in lung cancer cells. It was identified that miR-1301-3p is an upregulated miRNA in lung cancer via analyzing previously published microarray and The Cancer Genome Atlas-lung squamous cell carcinoma project data, and the upregulation of miR-1301-3p was confirmed in collected clinical samples and cells. Inhibition of miR-1301-3p suppressed lung cancer cell proliferation and migration. In addition, miR-1301-3p inhibition upregulated E-cadherin, an epithelial cell maker, and downregulated vimentin, a mesenchymal cell marker. Using bioinformatics analysis, it was revealed that polymerase I and transcript release factor (PTRF) is a target of miR-1301-3p. RT-qPCR, western blotting and dual-luciferase reporter assays demonstrated that PTRF is targeted by miR-1301-3p in lung cancer cells. The rescue experiments indicated that silencing PTRF could attenuate the inhibition of cell proliferation and migration induced by miR-1301-3p inhibitor in lung cancer cells. Furthermore, a strong negative correlation between miR-1301-3p and PTRF mRNA was identified in clinical samples. In summary, the present data highlight the involvement of miR-1301-3p in the proliferation and migration of lung cancer cells, indicating that miR-1301-3p may be a promising biomarker for lung cancer.

RETRACTED ARTICLE: Has_circ_0008583 modulates hepatocellular carcinoma progression through the miR-1301-3p/METTL3 pathway

“…In this study, miR-1301-3p was predicted to be a target of hsa_circ_0008583, which was confirmed by the luciferase reporter assay. Studies have shown that miR-1301-3p plays a dual role in tumor progression and can be used as a prognostic biomarker and therapeutic target for some malignant tumors [ 21–24 ]. In addition, miR-1301-3p was sponged by Circ_0004370, CircRNA MYLK and participated in cancer progression [ 25 , 26 ].…”

miR-1301-3p is a Potential Prognostic Biomarker for Esophageal Carcinoma and Acts by Downregulating NBL1 Expression

“…MiR-1301-3p is located on chr2:651-674 and is an important member of the miRNA family that is differentially expressed between malignant tumor and adjacent normal tissues. 20,22,23 As a result, miR-1301-3p has strongly been associated with invasion, migration, proliferation and apoptosis of tumor cells. For example, Zhi et al 20 suggested that miR-1301-3p suppressed cell proliferation by inducing G1/S phase arrest in human neuroglioma.…”

“…For example, Zhi et al 20 suggested that miR-1301-3p suppressed cell proliferation by inducing G1/S phase arrest in human neuroglioma. Wu et al 22 revealed that overexpression of miR-1301-3p in lung cancer cell signi cantly promoted growth and metastasis by targeting PTRF. In addition, Bi et al 23 indicated that miR-1301-3p targets PPP2R2C to promote prostate cancer cell growth.…”

Aberrant Expression of miR-1301 in Human Cancer

“…In four lung cancer cell lines, the expression of miR-1301 was higher than that of human normal lung epithelial cells (BEAS-2B) (5,21). Thy-1 is a cell surface glycoprotein, which is closely related to idiopathic pulmonary fibrosis (22) and lung cancer (5).…”

“…miRNA can target different downstream genes, and play an anti-cancer effect or carcinogenic effect in different tumors. Although miR-1301 is down-regulated in 9 tumors, miR-1301 is up-regulated in lung cancer ( 5 , 21 ) and breast cancer ( 3 , 20 ).…”