MiR-1303 Regulates Mycobacteria Induced Autophagy by Targeting Atg2B

Abstract

MicroRNAs are emerging post-transcriptional regulators of gene expressions in both innate immunity and adaptive immunity. In mycobacteria infection, autophagy plays an important role in innate defense mechanism and is tightly regulated by the autophagy-related proteins. Here, we show that Atg2B is involved in the regulation of mycobacteria-induced autophagy. MiR-1303, which function is not defined yet, is found to negatively regulate mycobacteria-induced Atg2B protein production, ultimately down-regulate mycobacteria-induced autophagy. MiR-1303 production is shown to be upregulated during BCG infection and its production is regulated by PI3K and NFκB. It is also demonstrated that miR-1303 targets putative target sites on Atg2B and possibly represses its translation.

Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells

“…Autophagy is tightly controlled by a family of autophagy regulators, autophagy-related proteins (Atgs), and their homologs and regulated by different signaling pathways such as mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K), and mammalian target of rapamycin (mTOR), 17 , 18 which coordinate autophagy through regulating autophagosome formation and autophagosome-lysosome fusion. In this study, we screened and identified miRNAs from Salvia miltiorrhiza and investigated whether and, if so, how the identified miRNA regulates autophagy in angiotensin II (Ang II)-stimulated VSMCs and mouse AAA models.…”

Evaluation of microRNA Expression in Patients with Herpes Zoster

“…Gene ontology (GO) analysis revealed that some of the identified target genes participate in the development of the immune and nervous systems (Table 4). For instance, miR-190b [19] and miR-1303 [20] regulate the expression of neuronal growth regulator 1 (NEGR1) [21], which belongs to an adhesion proteins family and has an important role in neurite outgrowth during the process of neuron development. It is also possible that regulation of this process is probably related to neuropathic pain resulting from HZ, however the specific mechanism remains unknown.…”

MicroRNA‐130a inhibits proliferation of vascular smooth muscle cells by suppressing autophagy via ATG2B

“…And our studies have confirmed that ATG2B is a direct target of miR‐130a by luciferase assays. Meanwhile, miR‐143 targets ATG2B to inhibit autophagy and increase inflammatory reactions in Crohn's disease, and miR‐1303 also regulates mycobacteria‐induced autophagy by targeting ATG2B, 38,39 which indicated that ATG2B is the direct target of miR‐130a to regulate autophagy in VSMCs.…”