MiR-132 Inhibits Expression of SIRT1 and Induces Pro-inflammatory Processes of Vascular Endothelial Inflammation through Blockade of the SREBP-1c Metabolic Pathway

Zhang, Liwei; Huang, Dangsheng; Wang, Qiushuang; Shen, Dong; Wang, Yumei; Chen, Bingyang; Zhang, Jinqian; Li, Gai

doi:10.1007/s10557-014-6533-x

Cited by 62 publications

(38 citation statements)

References 33 publications

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“…The impacts of NGR1 on miRNA expression might be via both genomic (transcriptional) and non-genomic mechanisms of action. Furthermore, we selected miR-132 for use in the follow-up rescue assays, since miR-132 has been reported to promote TNF-α-induced inflammation and cell death of HUVECs [34]. Besides, Kumarswamy and his colleagues have mentioned that deletion of miR-212/132 cluster increased endothelial vasodilatory function [35].…”

Section: Cellular Physiology and Biochemistry Cellular Physiology Andmentioning

confidence: 99%

Notoginsenoside R1 Protects HUVEC Against Oxidized Low Density Lipoprotein (Ox-LDL)-Induced Atherogenic Response via Down-Regulating miR-132

Fu¹,

Yin²,

Nie³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Radix notoginseng is a well-known traditional Chinese herbal medicine, has extensively pharmacological activities in cardiovascular system. Notoginsenoside R1 (NGR1) is one main active ingredient of Radix notoginseng. The purpose of this study was to evaluate the functional effects of NGR1 on atherosclerosis (AS). Methods: Human umbilical vascular endothelial cells (HUVECs) were subjected to oxidized low density lipoprotein (ox-LDL), before which cells were preconditioned with NGR1. Cell Counting Kit-8 (CCK-8) assay, flow cytometry, Transwell assay, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were carried out to assess the impacts of ox-LDL and NGR1 on HUVECs. Besides, the expression of microRNA-132 (miR-132), and the regulatory role of miR-132 in Matrix Gla Protein (MGP) expression were measured by qRT-PCR and Western blot. Results: NGR1 pre-conditioning prevented ox-LDL-induced apoptosis, migration and overproduction of Monocyte Chemoattractant Protein 1 (MCP-1) and Intercellular Adhesion Molecule 1 (ICAM-1). miR-132 was up-regulated in response to ox-LDL while was down-regulated by NGR1 pre-conditioning. The protective actions of NGR1 in ox-LDL-treated HUVECs were enhanced by miR-132 inhibitor, while were attenuated by miR-132 mimic. Besides, the up-regulated miR-132 could further decrease the expression of MGP, which acted as an anti-migratory and anti-adhesive factor. Furthermore, ox-LDL-induced the activation of c-Jun N-terminal Kinase (JNK) and Nuclear Factor Kappa B (NF-κB) pathways were partially attenuated by NGR1, and were fully eliminated by NGR1 treatment together with MGP overexpression. Conclusion: NGR1 prevents ox-LDL-induced apoptosis, migration and adhesion-related molecule release in HUVECs possibly via down-regulating miR-132, and subsequent up-regulating MGP.

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Section: Cellular Physiology and Biochemistry Cellular Physiology Andmentioning

confidence: 99%

Notoginsenoside R1 Protects HUVEC Against Oxidized Low Density Lipoprotein (Ox-LDL)-Induced Atherogenic Response via Down-Regulating miR-132

Fu¹,

Yin²,

Nie³

et al. 2018

Cell Physiol Biochem

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“…In addition, miR-19b plays a key role in the attenuation of TNF- α -induced EC apoptosis, and this function is closely linked to the Apaf1/caspase-7-dependent pathway [22]. Nevertheless, miR-132 promoted apoptosis of HUVEC induced by TNF- α and inhibited its proliferation, viability, and migration by inhibiting SIRT1 [23]. …”

Section: Mirnas and Vascular Agingmentioning

confidence: 99%

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

Lin

Zhan

Wang

et al. 2016

BioMed Research International

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Vascular aging, a specific type of organic aging, is related to age-dependent changes in the vasculature, including atherosclerotic plaques, arterial stiffness, fibrosis, and increased intimal thickening. Vascular aging could influence the threshold, process, and severity of various cardiovascular diseases, thus making it one of the most important risk factors in the high mortality of cardiovascular diseases. As endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) are the main cell biological basis of these pathology changes of the vasculature, the structure and function of ECs and VSMCs play a key role in vascular aging. MicroRNAs (miRNAs), small noncoding RNAs, have been shown to regulate the expression of multiple messenger RNAs (mRNAs) posttranscriptionally, contributing to many crucial aspects of cell biology. Recently, miRNAs with functions associated with aging or aging-related diseases have been studied. In this review, we will summarize the reported role of miRNAs in the process of vascular aging with special emphasis on EC and VSMC functions. In addition, the potential application of miRNAs to clinical practice for the diagnosis and treatment of cardiovascular diseases will also be discussed.

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“…miR-214 overexpression resulted in the inhibition of HUVEC migration, sprout formation and tubule formation on Matrigel ® in vitro , whereas anti-miR-214 induced the opposite effects. Interestingly, EC proliferation and viability were not affected by modulating miR-214 levels [77]. …”

Section: Mechanisms Of Mirnas In the Regulation Of Normal Cardiovascumentioning

confidence: 99%

“…Upregulation of miR-132 increases cell migration and viability, proliferation, and induces apoptosis in HUVECs. miR-132 also promotes TNFα-induced proinflammatory processes of ECs [77]. …”

Section: Mechanisms Of Mirnas In the Regulation Of Normal Cardiovascumentioning

confidence: 99%

Mechanisms and therapeutic potential of microRNAs in hypertension

Shi

Liao

Liu

et al. 2015

Drug Discovery Today

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Hypertension is the major risk factor for the development of stroke, coronary artery disease, heart failure and renal disease. The underlying cellular and molecular mechanisms of hypertension are complex and remain largely elusive. MicroRNAs (miRNAs) are short, noncoding RNA fragments of 22–26 nucleotides and regulate protein expression post-transcriptionally by targeting the 3′-untranslated region of mRNA. A growing body of recent research indicates that miRNAs are important in the pathogenesis of arterial hypertension. Herein, we summarize the current knowledge regarding the mechanisms of miRNAs in cardiovascular remodeling, focusing specifically on hypertension. We also review recent progress of the miRNA-based therapeutics including pharmacological and nonpharmacological therapies (such as exercise training) and their potential applications in the management of hypertension.

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MiR-132 Inhibits Expression of SIRT1 and Induces Pro-inflammatory Processes of Vascular Endothelial Inflammation through Blockade of the SREBP-1c Metabolic Pathway

Cited by 62 publications

References 33 publications

Notoginsenoside R1 Protects HUVEC Against Oxidized Low Density Lipoprotein (Ox-LDL)-Induced Atherogenic Response via Down-Regulating miR-132

Notoginsenoside R1 Protects HUVEC Against Oxidized Low Density Lipoprotein (Ox-LDL)-Induced Atherogenic Response via Down-Regulating miR-132

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

Mechanisms and therapeutic potential of microRNAs in hypertension

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