MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells

Xu, Panpan; Jia, Shuangkai; Wang, Kai; Fan, Zusen; Zheng, Hongqing; Lv, Jiangman; Jiang, Yanfen; Hou, Yong; Lou, Bihao; Zhou, Haoran; Zhang, Yanming; Guo, Kangkang

doi:10.1080/21505594.2020.1735051

Cited by 19 publications

(14 citation statements)

References 41 publications

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“…miRNA is closely related with viral infection. Viral infection alters host miRNA expression, and in turn, host miRNA regulates viral infection (Trobaugh and Klimstra 2017;Lin et al 2020;Wang et al 2019;Xu et al 2020). Most multicellular organisms and several viruses can generate miRNAs (Andreassen and Høyheim 2017a;Gutkoska et al 2017;Liu et al 2017b;Wang and Zhu 2017;Yang et al 2017a;Yu et al 2017;Huang et al 2019;Yoon et al 2020;Zhao et al 2020).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA regulation of viral replication in teleost fish: a review

Zhang

2021

Reviews in Aquaculture

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MicroRNAs (miRNAs) are single-stranded non-coding RNA molecules. In addition to participating in various biological processes of eukaryotes, such as cell proliferation, development, differentiation, metabolism, tumorigenesis, and apoptosis, miRNAs also play an important role in the regulation of virus replication. Here, we review the current knowledge on high-throughput miRNA sequencing of teleost fish or fish cells in response to virus infection, the role of teleost fish miRNAs in regulating virus replication via targeting either fish or virus genes and the function of teleost fish virus-encoded miRNAs during virus infection. Understanding the interaction between the miRNAs and the host or virus genes will lead to the identification of novel strategy to prevent virus infection in teleost fish.

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Section: Introductionmentioning

confidence: 99%

MicroRNA regulation of viral replication in teleost fish: a review

Zhang

2021

Reviews in Aquaculture

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“…The wild-type (WT) plasmids of circ_0016760 (circ_0016760-WT) and HIF1A (HIF1A-WT) and their mutant (MUT) plasmids (circ_0016760-MUT and HIF1A-MUT) were constructed by Ribobio Co., Ltd. Cell transfection was performed according to the literature’s methods [ 25 ]. Luciferase activities were detected using a Dual-Lucy Assay Kit (Solarbio).…”

Section: Methodsmentioning

confidence: 99%

PESV represses non-small cell lung cancer cell malignancy through circ_0016760 under hypoxia

Zhang

Zhu

et al. 2021

Cancer Cell Int

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Background Non-small cell lung cancer (NSCLC) accounts for more than 80% of lung cancers, which is the most common malignant tumor worldwide. Polypeptide extract from scorpion venom (PESV) has been reported to inhibit NSCLC process. The present study aims to reveal the roles of PESV in NSCLC progression under hypoxia and the inner mechanism. Methods The expression levels of circular RNA 0016760 (circ_0016760) and microRNA-29b (miR-29b) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was determined by western blot and immunohistochemistry assays. Cell migration, invasion, proliferation and tube formation were investigated by transwell, cell colony formation, 3-(4,5-Dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide and tube formation assays. The impacts between PESV and circ_0016760 overexpression on tumor growth in vivo were investigated by in vivo tumor formation assay. Results Circ_0016760 expression was dramatically upregulated in NSCLC tissues and cells, compared with adjacent lung tissues and cells, respectively. PESV treatment downregulated circ_0016760 expression. Circ_0016760 silencing or PESV treatment repressed cell migration, invasion, proliferation and tube formation under hypoxia in NSCLC cells. Circ_0016760 overexpression restored the effects of PESV treatment on NSCLC process under hypoxia. Additionally, circ_0016760 acted as a sponge of miR-29b, and miR-29b bound to HIF1A. Meanwhile, miR-29b inhibitor impaired the influences of circ_0016760 knockdown on NSCLC process under hypoxia. Further, ectopic circ_0016760 expression restrained the effects of PESV exposure on tumor formation in vivo. Conclusion Circ_0016760 overexpression counteracted PESV-induced repression of NSCLC cell malignancy and angiogenesis under hypoxia through miR-29b/HIF1A axis.

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“…These host proteins could be divided into the following categories according to their possible mechanism involved in CSFV replication. (1) Many members of the small Rab GTPase family, such as Rab1A [58], Rab1b [59], Rab2 [60], Rab5 [61,62], Rab7, Rab11 [62], Rab18 [63], and Rab25 [64], play a critical role in CSFV replication. For example, Rab18 interacts with CSFV NS5A and mediates viral RNA replication and virion assembly [63].…”

Section: Host Proteins Whose Function Defect Exert Anti-csfv Effectsmentioning

confidence: 99%

“…MiRNAs could also regulate CSFV replication. For example, miR-140 inhibits CSFV replication by targeting Rab25 [64]. However, there are still few studies on the relationship between miRNA and CSFV replication.…”

Section: Concluding Remarks and Prospectsmentioning

confidence: 99%

Anti-Classical Swine Fever Virus Strategies

et al. 2021

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Classical swine fever (CSF), caused by CSF virus (CSFV), is a highly contagious swine disease with high morbidity and mortality, which has caused significant economic losses to the pig industry worldwide. Biosecurity measures and vaccination are the main methods for prevention and control of CSF since no specific drug is available for the effective treatment of CSF. Although a series of biosecurity and vaccination strategies have been developed to curb the outbreak events, it is still difficult to eliminate CSF in CSF-endemic and re-emerging areas. Thus, in addition to implementing enhanced biosecurity measures and exploring more effective CSF vaccines, other strategies are also needed for effectively controlling CSF. Currently, more and more research about anti-CSFV strategies was carried out by scientists, because of the great prospects and value of anti-CSFV strategies in the prevention and control of CSF. Additionally, studies on anti-CSFV strategies could be used as a reference for other viruses in the Flaviviridae family, such as hepatitis C virus, dengue virus, and Zika virus. In this review, we aim to summarize the research on anti-CSFV strategies. In detail, host proteins affecting CSFV replication, drug candidates with anti-CSFV effects, and RNA interference (RNAi) targeting CSFV viral genes were mentioned and the possible mechanisms related to anti-CSFV effects were also summarized.

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MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells

Cited by 19 publications

References 41 publications

MicroRNA regulation of viral replication in teleost fish: a review

MicroRNA regulation of viral replication in teleost fish: a review

PESV represses non-small cell lung cancer cell malignancy through circ_0016760 under hypoxia

Anti-Classical Swine Fever Virus Strategies

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