miR-142-3p is essential for hematopoiesis and affects cardiac cell fate in zebrafish

Nishiyama, Takahiko; Kaneda, Ruri; Ono, Tomohiko; Tohyama, Shugo; Hashimoto, Hisayuki; Endo, Jin; Tsuruta, Hikaru; Yuasa, Shinsuke; Ieda, Masaki; Makino, Shinji; Fukuda, Keiichi

doi:10.1016/j.bbrc.2012.07.148

Cited by 39 publications

(36 citation statements)

References 23 publications

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“…Our conclusion is in agreement with observations by others that miR-142-3p and not miR-142-5p is responsible for the regulation of immune cell function in mice 12,13,35 and zebrafish. [9][10][11] Growing evidence suggests that miRNAs tightly control cellular responses and organismal homeostasis by targeting signaling nodes in a variety of physiological networks. In agreement with this notion, our study shows that miR-142-3p targets several signaling pathways that play well-established roles in B-cell differentiation and function, including the VDJ recombination complex, actin cytoskeleton rearrangement networks, and BAFF-R signaling cascade.…”

Section: Discussionmentioning

confidence: 99%

“…Knockdown of miR-142-3p expression in zebrafish revealed a critical role for this miRNA in hematopoiesis during embryonic development, hematopoietic stem cell homeostasis, neutrophil differentiation, and heart development. [9][10][11] In mice, miR-142 is essential for normal development and function of megakaryocytes, dendritic, and mast cells.…”

Section: Introductionmentioning

confidence: 99%

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Altered lymphopoiesis and immunodeficiency in miR-142 null mice

Kramer¹,

Wang²,

Reyes³

et al. 2015

Blood

102

118

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Altered lymphopoiesis and immunodeficiency in miR-142 null mice

Kramer¹,

Wang²,

Reyes³

et al. 2015

Blood

102

118

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“…Consistent with the ratio of the miR-K10a 5 ′ -isomiRs detected by deep sequencing (Supplemental Table S2; Gottwein et al 2011), the miRK10a 5 ′ -isomiR is slightly more abundant in PEL cell lines than miR-K10a+1. Because of the emerging pivotal role of miR-142-3p in the vertebrate hematopoietic lineage (Nishiyama et al 2012;Mildner et al 2013;Nimmo et al 2013;Chapnik et al 2014), we assessed whether the expression of mature miR-142-3p−1 is conserved beyond mice and humans. The predicted mature miR-142-3p/−1 sequences are invariant across vertebrates (http://genome.ucsc .edu/), but less conserved portions of the pri-miRNA could lead to differences in isomiR expression.…”

Section: Mir-142-3p 5 ′ -Isomir Expression Is Conserved In Vertebratesmentioning

confidence: 99%

“…Overexpression of the miR-142 precursor in mouse hematopoietic progenitor cells substantially increases the T-cell population in vitro (Chen et al 2004). The inactivation of miR-142-3p causes defects in hematopoiesis in zebrafish (Nishiyama et al 2012) and prevents the specification of definitive hemangioblasts in Xenopus (Nimmo et al 2013). In mice, ablation of the miR-142 locus results in reduced numbers of CD4 + dendritic cells (Mildner et al 2013) and a severe impairment of platelet formation (Chapnik et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Divergent target recognition by coexpressed 5′-isomiRs of miR-142-3p and selective viral mimicry

Manzano

Forte

Raja

et al. 2015

RNA

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Sequence heterogeneity at the ends of mature microRNAs (miRNAs) is well documented, but its effects on miRNA function are largely unexplored. Here we studied the impact of miRNA 5 ′ -heterogeneity, which affects the seed region critical for target recognition. Using the example of miR-142-3p, an emerging regulator of the hematopoietic lineage in vertebrates, we show that naturally coexpressed 5 ′ -variants (5 ′ -isomiRs) can recognize largely distinct sets of binding sites. Despite this, both miR-142-3p isomiRs regulate exclusive and shared targets involved in actin dynamics. Thus, 5′ -heterogeneity can substantially broaden and enhance regulation of one pathway. Other 5 ′ -isomiRs, in contrast, recognize largely overlapping sets of binding sites. This is exemplified by two herpesviral 5 ′ -isomiRs that selectively mimic one of the miR-142-3p 5 ′ -isomiRs. We hypothesize that other cellular and viral 5 ′ -isomiRs can similarly be grouped into those with divergent or convergent target repertoires, based on 5 ′ -sequence features. Taken together, our results provide a detailed characterization of target recognition by miR-142-3p and its 5 ′ -isomiR-specific viral mimic. We furthermore demonstrate that miRNA 5 ′ -end variation leads to differential targeting and can thus broaden the target range of miRNAs.

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“…miR-142-3p can directly regulate the expression of tab2 in the myeloid lineage [22] and that of IL-6 in dendritic cells(DCs) [23]. Very recently, zebrafish miR-142-3p (ZFIN ID: ZDB-GENE-090929-151, miR-142a-3p herein) has been shown to be required for the maturation of primitive erythrocytes, cardiogenesis and vessel integrity [24,25]. However, the mechanisms underlying miRNA regulation of HSC development in vivo during vertebrate embryogenesis remain largely unclear.…”

Section: Introductionmentioning

confidence: 99%

miR-142-3p regulates the formation and differentiation of hematopoietic stem cells in vertebrates

et al. 2013

Cell Res

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Previous studies on developmental hematopoiesis have mainly focused on signaling and transcription factors, while the appreciation of epigenetic regulation including that of microRNAs is recent. Here, we show that in zebrafish and mouse, miR-142-3p is specifically expressed in hematopoietic stem cells (HSCs). Knockdown of miR-142a-3p in zebrafish led to a reduced population of HSCs in the aorta-gonad-mesonephros (AGM) region as well as T-cell defects in the thymus. Mechanistically, miR-142a-3p regulates HSC formation and differentiation through the repression of interferon regulatory factor 7 (irf7)-mediated inflammation signaling. Finally, we show that miR-142-3p is also involved in the development of HSCs in mouse AGM, suggesting that it has a highly conserved role in vertebrates. Together, these findings unveil the pivotal roles that miR-142a-3p plays in the formation and differentiation of HSCs by repressing irf7 signaling.

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miR-142-3p is essential for hematopoiesis and affects cardiac cell fate in zebrafish

Cited by 39 publications

References 23 publications

Altered lymphopoiesis and immunodeficiency in miR-142 null mice

Altered lymphopoiesis and immunodeficiency in miR-142 null mice

Divergent target recognition by coexpressed 5′-isomiRs of miR-142-3p and selective viral mimicry

miR-142-3p regulates the formation and differentiation of hematopoietic stem cells in vertebrates

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