miR-143 suppresses osteogenic differentiation by targeting Osterix

Li, Enqi; Zhang, Jinli; Yuan, Tianxiang; Ma, Baotong

doi:10.1007/s11010-013-1957-3

Cited by 69 publications

(53 citation statements)

References 21 publications

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“…Li et al reported that miR-143 suppressed osteogenic differentiation by targeting Osx in osteoblastic MC3T3-E1 cells [84]. MiR-143-mimic transfection upregulated Osx , alkaline phosphatase (ALP) and osteocalcin levels, whereas miR-143 inhibitor reduced their expression.…”

Section: The Role Of Mirnas In Osteoblastsmentioning

confidence: 99%

MicroRNAs involved in bone formation

Papaioannou

Mirzamohammadi

Kobayashi

2014

Cell. Mol. Life Sci.

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During skeletal development, mesenchymal progenitor cells undergo a multistage differentiation process in which they proliferate and become bone- and cartilage-forming cells. This process is tightly regulated by multiple levels of regulatory systems. The small non-coding RNAs, microRNAs (miRNAs), post-transcriptionally regulate gene expression. Recent studies have demonstrated that miRNAs play significant roles in all stages of bone formation, suggesting the possibility that miRNAs can be novel therapeutic targets for skeletal diseases. Here, we review the role and mechanism of action of miRNAs in bone formation. We discuss roles of specific miRNAs in major types of bone cells, osteoblasts, chondrocytes, osteoclasts, and their progenitors. Except a few, the current knowledge about miRNAs in bone formation has been obtained mainly by in vitro studies; further validation of these findings in vivo is awaited. We also discuss about several miRNAs of particular interest in the light of future therapies of bone diseases.

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Section: The Role Of Mirnas In Osteoblastsmentioning

confidence: 99%

MicroRNAs involved in bone formation

Papaioannou

Mirzamohammadi

Kobayashi

2014

Cell. Mol. Life Sci.

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“…It was recently revealed that miRNAs have been involved in mediating the osteogenic differentiation of osteoblasts and mesenchymal stem cells (MSCs). MiR-143 and miR-145 were decreased in osteogenic differentiation, and their overexpression suppressed osteogenic differentiation by targeting Osterix and Sp7 transcription factor 7 (Sp7) in vitro, respectively [12,13]. MiR-155 is induced by Interferon-β and inhibits osteoclast differentiation by targeting suppressor of cytokine signaling 1 (Socs1) and microphthalmia-associated transcription factor (Mitf), two essential regulators of osteoclastogenesis [14].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia-Induced MicroRNA-429 Promotes Differentiation of MC3T3-E1 Osteoblastic Cells by Mediating ZFPM2 Expression

Huang

Peng

Cao

et al. 2016

Cell Physiol Biochem

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Background/Aims: Hypoxia has been reported to regulate osteoblastic differentiation of bone cells and cartilage development. However, information concerning the molecular mechanisms remains largely unknown. Methods: The expression of miR-429 was evaluated by quantitative real-time PCR analysis. To test whether miR-429 directly regulate the expression level of ZFPM2 at transcription level, dual-luciferase reporter gene assay was performed. Western blotting was performed to detect osteogenesis related protein expression. The cell proliferation, apoptosis, alkaline phosphatase activity and matrix mineralization were performed to assess the functions of miR-429 in vitro and in vivo the effects of miR-429 on fracture healing. Results: Expression of miR-429 was increased in MC3T3-E1 cells treated with 200 μM CoCl₂ by qRT-PCR, and overexpression of miR-429 promoted cell differentiation, and enhanced alkaline phosphatase activity and matrix mineralization. Luciferase reporter assays suggested that miR-429 directly targets the 3'UTR of ZFPM2. In addition, knockdown of ZFPM2 could phenocopy the effects of miR-429 expression. Furthermore, overexpression of ZFPM2 in miR-429-expressing MC3T3-E1 cells suppressed cell differentiation. Conclusions: Our results provide valuable insight into the potential role of hypoxia in regulation of osteoblastic cell differentiation.

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“…However, a relationship has not been confirmed beyond the finding of reduced Osx mRNA [99]. In contrast, miR-143 [100,101], miR-145 [100], miR-214 [102] and miR-637 [103] suppress osteogenic differentiation by directly targeting Osx. Due to the ability of miR-322 to directly target Tob2 , which normally helps to degrade Osx, Osx mRNA is stabilized, allowing osteoblast differentiation to occur [104].…”

Section: Micrornas Associated With the Mesenchymal State And Mesenchymentioning

confidence: 99%

microRNA Regulation of Skeletal Development

Sera

Nieden

2017

Curr Osteoporos Rep

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Purpose of review Osteogenesis is a complex process involving the specification of multiple progenitor cells and their maturation and differentiation into matrix-secreting osteoblasts. Osteogenesis occurs not only during embryogenesis, but also during growth, after an injury, and in normal homeostatic maintenance. While much is known about osteogenesis associated regulatory genes, the role of microRNAs, which are epigenetic regulators of protein expression, is just beginning to be explored. While miRNAs do not abrogate all protein expression, their purpose is to finely tune it, allowing for a timely and temporary protein down-regulation. Recent findings The last decade has unveiled a multitude of microRNAs that regulate key proteins within the osteogenic lineage, thus qualifying them as ‘osteomiRs’. These miRNAs may endogenously target an activator or inhibitor of differentiation, and depending on the target, may either lead to the prolongation of a progenitor maintenance state or to early differentiation. Interestingly, cellular identity seems intimately coupled to the expression of miRNAs, which participate in the suppression of previous and subsequent differentiation steps. In such cases where key osteogenic proteins were identified as direct targets of miRNAs in non-bone cell types, or through bioinformatic prediction, future research illuminating the activity of these miRNAs during osteogenesis will be extremely valuable. Summary Many bone related diseases involve the dysregulation of transcription factors or other proteins found within osteoblasts and their progenitors, and the dysregulation of miRNAs, which target such factors, may play a pivotal role in disease etiology, or even as a possible therapy.

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miR-143 suppresses osteogenic differentiation by targeting Osterix

Cited by 69 publications

References 21 publications

MicroRNAs involved in bone formation

MicroRNAs involved in bone formation

Hypoxia-Induced MicroRNA-429 Promotes Differentiation of MC3T3-E1 Osteoblastic Cells by Mediating ZFPM2 Expression

microRNA Regulation of Skeletal Development

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