miR-146a and miR-638 in BRCA1-deficient triple negative breast cancer tumors, as potential biomarkers for improved overall survival

Zavala, Valentina A.; Pérez-Moreno, Elisa; Tapia, Teresa; Camus, Mauricio; Carvallo, Pilar

doi:10.3233/cbm-150545

Cited by 32 publications

(19 citation statements)

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“…miR-146a precursor SNP (rs2910164) involves a G>C nucleotide substitution that changes a G:U pair to a C:U (11). Higher expression of miR-146a was observed in many solid types of cancer, such as melanoma, breast and lung cancer (12)(13)(14). A particular miR-146a was found to promote cellular viability (15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

miR-146a promotes cervical cancer cell viability via targeting IRAK1 and TRAF6

Song

Ding

et al. 2018

Oncol Rep

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Cervical cancer is the third most common type of cancer in women, and microRNAs play an important role in this type of cancer. The elevated expression of miR-146a is involved in the pathogenesis of cancers generally, but its role in cervical cancer has not been fully elucidated. In the present study, we assessed the expression of miR-146a in G>C polymorphisms and confirmed that the overexpression of miR-146a promoted cervical cancer cell viability. The recombinant expression plasmids pre-miR-146a-G or pre-miR-146a-C including single nucleotide polymorphisms (SNP) were successfully constructed. Pre-miR-146a-G or pre-miR-146a-C was transfected into cervical cancer cells or immortalized non-tumorigenic cells and the expression of miR-146a was evaluated by real-time PCR. The cell viability, cell-cycle analysis and apoptosis were assessed using Cell Counting Kit-8 assay (CCK-8), flow cytometry and cleaved caspase-3 protein expression, respectively. The expression of interleukin 1 receptor associated kinase 1 (IRAK1), TNF receptor-associated factor 6 (TRAF6) and cyclin D1 was assessed following the transfection with a miR-146a mimic or a negative control. The cell viability and the number of S-phase cells increased after transfection with miR-146a mimic or an IRAK1 or TRAF6 interference fragment. After transfection, IRAK1 and TRAF6 protein expression was downregulated and the expression of cyclin D1 was upregulated, however apoptosis and cleaved caspase-3 were not affected. Polymorphisms in miR-146a precursor may be linked to the expression of miR-146a and may be a potential target for cervical cancer therapy.

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Section: Introductionmentioning

confidence: 99%

miR-146a promotes cervical cancer cell viability via targeting IRAK1 and TRAF6

Song

Ding

et al. 2018

Oncol Rep

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“…Zavala et al illustrated miR‐146a and miR‐638 were overexpressed TNBC. Furthermore, expression of both miRNAs in this patients is linked in BRCA1 expression and overexpression of miR‐146a and miR‐638 was linked in a favorable OS relative to the group with normal BRCA1 and low‐expression of both miRNAs (Zavala, Pérez‐Moreno, Tapia, Camus, & Carvallo, ). Accumulating evidence suggest that detecting breast cancer in early‐stage involved in favorable disease outcome as well as prolonged patient survival.…”

Section: Micrornas Can Be New Biomarkers In the Diagnosis Of Breast Tmentioning

confidence: 84%

“…Moreover, the upregulation of miR-301a correlated with poor prognosis in TNBC. (Yu et al, 2014;Zavala et al, 2016) Abbreviations expression of 804 miRNAs in 12 inflammatory breast tumor compared with 31 non-IBC and eight healthy samples. Many studies confirmed that mir-9, mir-155, mir-122, and mir-27b are miRNAs that were linked in unfavorable prognosis in breast cancer (Kong et al, 2014;Shen et al, 2014;Wu, Somlo et al, 2012;Zhou et al, 2012).…”

Section: Prognosis Tissuementioning

confidence: 99%

MicroRNAs in breast cancer: Roles, functions, and mechanism of actions

Abolghasemi

Tehrani

Yousefi

et al. 2019

Journal Cellular Physiology

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Breast cancer is one of the most lethal malignancies in women in the world. Various factors are involved in the development and promotion of the malignancy; most of them involve changes in the expression of certain genes, such as microRNAs (miRNAs). MiRNAs can regulate signaling pathways negatively or positively, thereby affecting tumorigenesis and various aspects of cancer progression, particularly breast cancer. Besides, accumulating data demonstrated that miRNAs are a novel tool for prognosis and diagnosis of breast cancer patients. Herein, we will review the roles of these RNA molecules in several important signaling pathways, such as transforming growth factor, Wnt, Notch, nuclear factor‐κ B, phosphoinositide‐3‐kinase/Akt, and extracellular‐signal‐regulated kinase/mitogen activated protein kinase signaling pathways in breast cancer.

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“…miR-146a plays important roles in the development and progression of many types of tumors, and it has been widely studied and reported in recent years. The expression of miR-146a is decreased significantly in breast (26,27), liver (28,29), prostate (30) and bladder cancer (31), and upregulation of miR-146a can inhibit the proliferation, invasion, and metastasis of these tumor cells and promote apoptosis (26-31); Figure 6. Detection of miR-146a targeted genes using a dual-luciferase reporter assay system.…”

Section: Discussionmentioning

confidence: 99%

Effect and mechanism of miR‑146a on malignant biological behaviors of lung adenocarcinoma cell line

et al. 2020

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The aim of the present study was to assess the expression of microRNA-146a (miR-146a) in human lung adenocarcinoma cells, its effect on cellular behaviors, and the underlying molecular mechanisms. Reverse transcription-quantitative PCR (RT-qPCR) was used to measure miR-146a expression in the human normal lung epithelial cell line, BEAS-2B, and human lung adenocarcinoma cell lines, A549, PC-9 and H1299, to determine whether miR-146a acts as an oncogene or anti-oncogene. miR-146a mimics were transfected into target cells to observe the proliferation, apoptosis, invasion and migration of human lung adenocarcinoma cells. The target genes of miR-146a were predicted using bioinformatics analysis, and binding sites were validated by dual-luciferase reporter assay. Target gene expression at the mRNA and protein levels was measured by RT-qPCR and western blot analysis, respectively. The expression levels of miR-146a in human lung adenocarcinoma cell lines were lower than its expression in BEAS-2B (P<0.01). A549 cell line is a EGFR wild-type lung adenocarcinoma cell line, which is also the most widely studied in NSCLC, and therefore this was chosen as the target cell line for further investigation. Overexpression of miR-146a in A549 cells can inhibit cell proliferation (P<0.05), promote apoptosis (P<0.05), and reduce the cells' migratory ability (P<0.01). Bioinformatics prediction indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) and TNF receptor associated factor 6 (TRAF6) are the target genes of miR-146a. Dual-luciferase reporter assay showed that miR-146a could specifically bind to 3'-untranslated regions of IRAK1 and TRAF6. The protein and mRNA levels of IRAK1 and TRAF6 were significantly downregulated after miR-146a overexpression in A549 cells (P<0.01). The results of this study demonstrated that the expression of miR-146a in human lung adenocarcinoma cells was significantly lower than in normal lung epithelial cells, indicating that miR-146a acts as an anti-oncogene. miR-146a suppresses the proliferation and migration of human lung adenocarcinoma cells by downregulating the expression of IRAK1 and TRAF6.

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miR-146a and miR-638 in BRCA1-deficient triple negative breast cancer tumors, as potential biomarkers for improved overall survival

Abstract: Both miRNAs are potential biomarkers for improved overall survival in patients with BRCA1-deficient TNBC tumors.

Cited by 32 publications

References 0 publications

miR-146a promotes cervical cancer cell viability via targeting IRAK1 and TRAF6

miR-146a promotes cervical cancer cell viability via targeting IRAK1 and TRAF6

MicroRNAs in breast cancer: Roles, functions, and mechanism of actions

Effect and mechanism of miR‑146a on malignant biological behaviors of lung adenocarcinoma cell line

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