miR-154* and miR-379 in the DLK1-DIO3 MicroRNA Mega-Cluster Regulate Epithelial to Mesenchymal Transition and Bone Metastasis of Prostate Cancer

Osteosarcoma is the most common primary bone tumour. Increasing evidence has demonstrated the pathogenic role of microRNA (miRNAs) dysregulation in tumour development. miR‐379 was previously reported to function as an oncogenic or tumour‐suppressing miRNA in a tissue‐dependent manner. However, its function in osteosarcoma remains unknown. In this study, we found that the expression of miR‐379 was downregulated in osteosarcoma tissues and cell lines. Further functional characterization revealed that miR‐379 suppressed osteosarcoma cell proliferation and invasion in vitro and retarded the growth of osteosarcoma xenografts in vivo. Mechanistically, PDK1 was identified as the direct target of miR‐379 in osteosarcoma, in which PDK1 expression was up‐regulated and showed inverse correlation with miR‐379. Enforced expression of PDK1 promoted osteosarcoma cell proliferation and rescued the anti‐proliferative effect of miR‐379. These data suggest that miR‐379 could function as a tumour‐suppressing miRNA via targeting PDK1 in osteosarcoma.

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“…vPrevious studies showed that aberrant expression of miR‐379 contributes to cancer development 31, 32, 33. For instance, Khan et al .…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1

Shen

Chan

et al. 2016

J Cellular Molecular Medi

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“…It is important to explore the molecular mechanisms underlying the initiation and development of cervical cancer. A growing amount of evidence has demonstrated that miRNAs are important regulators of various types of biological processes in cancers, including cell proliferation, cell cycle, apoptosis, invasion and migration (24)(25)(26). Furthermore, the abnormal expression of miRNAs is associated with carcinogenesis and progression of cancer (27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-10b inhibits proliferation, migration and invasion in cervical cancer cells via direct targeting of insulin-like growth factor-1 receptor

2017

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Abstract. MicroRNAs are deregulated in numerous types of human cancers and have crucial roles in the carcinogenesis and progression of human cancers. MicroRNA-10b (miR-10b) has been studied in several types of human cancer. However, the expression and roles of miR-10b in cervical cancer remain unknown. In the present study, the expression, functions and molecular mechanisms of miR-10b were explored in cervical cancer. The present data revealed that miR-10b was significantly downregulated in cervical cancer tissues and cell lines. In addition, miR-10b overexpression inhibited the proliferation, migration and invasion of cervical cancer cells, while miR-10b under-expression had the opposite effect. Based on bioinformatics analysis, a luciferase reporter assay and western blot analysis, insulin-like growth factor-1 receptor (IGF-1R) was identified as a direct target of miR-10b in cervical cancer. In addition, IGF-1R small interfering RNA-mediated knockdown of IGF-1R also inhibited the proliferation, migration and invasion of the cervical cancer cells. In conclusion, the present study demonstrated that miR-10b serves an important role in cervical cancer progression by targeting IGF-1R.

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“…In contrast, miR-154*, miR-379 and miR-409-3p/-5p promote tumourigenesis, EMTand bone metastasis of human prostate cancer. 33 Dicer is a target for miR-103/107, whose expression is associated with metastasis and poor outcome in breast cancer patients. 21 This miRNA family attenuates miRNA biosynthesis by regulating Dicer expression and induces EMT thus enhancing the migratory ability of the cells in vitro and the metastatic dissemination in vivo.…”

Section: Mirnas In Primary Carcinomas Are Potential Predictive Factormentioning

confidence: 99%

Tumour-derived miRNAs and bone metastasis

Croset

Kan

Clézardin³

2015

BoneKEy Reports

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Skeletal metastases are complications of epithelial cancers, among which breast, prostate and lung carcinomas are the most osteotropic. In primary tumours, a subset of cancer cells undergoes epithelial-mesenchymal transition, acquires mobility to migrate into the surrounding stroma and seeds at distant sites to grow. The specific development of bone metastasis requires the recruitment of circulating tumour cells in the bone marrow, their adaptation to survive in the surrounding microenvironment where they alter the functions of osteoclasts and osteoblasts, and hijack signals coming from the bone matrix. Each of the molecular pathways underlining these steps is regulated by multiple factors, through the tight control of genes expressed by cancer cells interacting with cells from the bone microenvironment. In this context, miRNAs can act as master regulators of gene expression to control multiple aspects of bone metastasis formation, including cancer cell escape from the primary tumour site, cancer cell dissemination to bone and invasion of the bone marrow, as well as secondary outgrowth and tumour-stroma cell interactions. In the clinic, specific miRNA signatures have been identified in osteotropic cancer cells, raising the possibility that miRNAs could be used as biomarkers of bone metastasis. The regulatory activity of miRNAs in the bone microenvironment also suggests that miRNAs could be promising therapeutic targets.

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miR-154* and miR-379 in the DLK1-DIO3 MicroRNA Mega-Cluster Regulate Epithelial to Mesenchymal Transition and Bone Metastasis of Prostate Cancer

Cited by 99 publications

References 42 publications

RETRACTED: MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1

RETRACTED: MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1

MicroRNA-10b inhibits proliferation, migration and invasion in cervical cancer cells via direct targeting of insulin-like growth factor-1 receptor

Tumour-derived miRNAs and bone metastasis

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