miR-154 suppresses non-small cell lung cancer growth in vitro and in vivo

Lin, Xingyu; Yang, Zhiyong; Zhang, Peng; Shao, Guoguang

doi:10.3892/or.2015.3895

Cited by 35 publications

(36 citation statements)

References 28 publications

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“…For NSCLC, a previous study by the present authors revealed that miR-154 expression is downregulated in human primary NSCLC tissues and cell lines, and that exogenous miR-154 significantly suppressed NSCLC growth in vitro and in vivo (20), suggesting that miR-154 has potential therapeutic application against NSCLC. However, the molecular mechanisms by which it exerts its functions remain largely unknown.…”

Section: Introductionmentioning

confidence: 53%

miR-154 inhibits migration and invasion of human non-small cell lung cancer by targeting ZEB2

et al. 2016

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Abstract. Emerging evidence suggests that microRNAs (miRs) play critical roles in the development and progression of non-small cell lung cancer (NSCLC). In a previous study, the present authors demonstrated that miR-154 acts as a tumor suppressor in NSCLC; however, its underlying molecular mechanism and target in NSCLC remain poorly understood. In the present study, ectopic expression of miR-154 remarkably suppressed cell migration and invasion in NSCLC cells.Zinc finger E-box binding homeobox 2 (ZEB2) was identified as a direct target of miR-154 in NSCLC cells. Furthermore, overexpression of miR-154 could decrease the expression of ZEB2 at the messenger RNA and protein levels. Ectopic expression of miR-154 also increased the levels of E-cadherin, an epithelial marker, and decreased the levels of vimentin, a mesenchymal marker, which contributed to suppress epithelial-mesenchymal transition (EMT) and to inhibit cell migration and invasion. In addition, downregulation of ZEB2 exerted similar effects to those caused by miR-154 overexpression on NSCLC cell migration and invasion, while upregulation of ZEB2 could significantly reverse the inhibitory effects on migration and invasion caused by miR-154 on NSCLC cells. These findings demonstrated that miR-154 inhibited migration and invasion of NSCLC cells by regulating EMT through targeting ZEB2, suggesting that miR-154 may be a potential anticancer therapeutic target for NSCLC.

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Section: Introductionmentioning

confidence: 53%

miR-154 inhibits migration and invasion of human non-small cell lung cancer by targeting ZEB2

et al. 2016

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“…Pang et al demonstrated that miR-154 expression was negatively correlated with tumor differentiation, tumor-node-metastasis (TNM) stage and lymph node metastasis in hepatocellular carcinoma (23). Lin et al revealed that low miR-154 expression was significantly correlated with metastasis, larger tumor size and advanced TNM stage in NSCLC (21). These findings implicated the potential effects of miR-154 in the prognosis of cancer.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑154 functions as a tumor suppressor in non‑small cell lung cancer through directly targeting B‑cell‑specific Moloney murine leukemia virus insertion site 1

et al. 2018

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Abstract. Lung cancer remains the leading cause of cancer-associated mortality in China and worldwide. Increasing numbers of studies have demonstrated that microRNAs (miRNAs/miRs) have vital functions in numerous developmental processes and tumorigenesis. The aim of the present study was to investigate miR-154 expression in non-small cell lung cancer (NSCLC), and to explore the roles of miR-154 in the carcinogenesis and progression of this cancer. Reverse transcription-polymerase chain reaction (RT-qPCR) was performed to detect miR-154 expression in NSCLC tissues and cell lines. In addition, cell proliferation assay, migration and invasion assays were adopted to investigate the functional roles of miR-154 in NSCLC. Bioinformatics analysis, luciferase reporter assay, RT-qPCR and western blot analysis were used to explore the potential targets of miR-154 in NSCLC. According to the results, miR-154 was significantly downregulated in NSCLC tissues and cell lines. Restoration of miR-154 expression inhibited proliferation, migration and invasion of NSCLC cells. In addition, B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI-1) was identified as a direct target gene of miR-154 in NSCLC. In conclusion, miR-154 may function as a tumor suppressor in NSCLC, partly by regulating BMI-1, and the modulation of miR-154 expression represents a potential strategy for the treatment of NSCLC patients. IntroductionLung cancer, a highly malignant tumor, is the leading cause of cancer-associated mortality in males and females worldwide (1). The incidence and mortality rates of non-small cell lung cancer are increasing in developing countries, including China, due to the increase in the use of tobacco as well as air pollution (2). According to its pathological pattern, lung cancer may be divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) (3). NSCLC, which includes adenocarcinoma, squamous cell carcinoma, adenosquamous cell carcinoma and large cell carcinoma, accounts for ~80-85% of all lung cancer cases (4). Despite advances in traditional treatments, including surgery, supplemented with radiotherapy and chemotherapy, the prognosis remains poor and the five-year overall survival rate is extremely low (17.1%) (5). These facts indicate an urgent requirement to fully understand the molecular mechanisms underlying the carcinogenesis and progression of NSCLC, and to investigate novel therapeutic targets to control this malignant disease.Increasing studies have demonstrated that microRNAs (miRNAs/miRs) have vital functions in numerous developmental processes and tumorigenesis, including the progression of NSCLC (6-8). miRNAs represent a group of small (~19-25 nucleotides), non-protein-coding, and endogenous single RNAs, which negatively regulate gene expression by binding to the 3' untranslated regions (3'UTRs) of target genes in an imperfect base pairing manner, causing mRNA cleavage or translational repression (9,10). To date, miRNAs have been demonstrated to regulate >30% of all cellular pro...

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“…In NSCLC, cells overexpression of miR-154 inhibits cell growth, colony formation, migration and invasion, and enhances cellular apoptosis and G0/G1 cell cycle arrest (21). Upregulation of miR-154 also suppresses the growth of NSCLC cell xenografts in vivo (21).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

Qiao¹,

Cao²,

Yang³

2017

Oncology Letters

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Abstract. MicroRNAs (miRNAs) are a group of non-protein-coding, highly conserved single-stranded RNA molecules. The abnormal expression of miRNAs has been demonstrated to have an important function in the carcinogenesis and progression of gastric cancer. microRNA-154 (miR-154) has been reported to be downregulated in non-small cell lung, colorectal and prostate cancer. However, the expression and roles of miR-154 in gastric cancer remain to be established. The present study measured the expression levels of miR-154 in gastric cancer tissues and cell lines. miR-154 was found to be significantly downregulated in gastric cancer tissues and cell lines. In addition, functional studies indicated that the overexpression of miR-154 inhibited the proliferation, migration and invasion of gastric cancer cells. Using TargetScan, a dual luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction and western blot analysis, metadherin (MTDH) was revealed as a novel miR-154 target. In addition, knocking down MTDH lead to a similar effect as overexpressing-154 in gastric cells. The present findings indicate that miR-154 was downregulated in gastric cancer, and inhibited tumor behaviors of gastric cancer cells partially through the downregulation of MTDH. Therefore, the miR-154/MTDH axis may be a novel therapeutic to treat patients with gastric cancer.

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miR-154 suppresses non-small cell lung cancer growth in vitro and in vivo

Cited by 35 publications

References 28 publications

miR-154 inhibits migration and invasion of human non-small cell lung cancer by targeting ZEB2

miR-154 inhibits migration and invasion of human non-small cell lung cancer by targeting ZEB2

MicroRNA‑154 functions as a tumor suppressor in non‑small cell lung cancer through directly targeting B‑cell‑specific Moloney murine leukemia virus insertion site 1

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

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