miR‐16 inhibits cell proliferation by targeting IGF1R and the Raf1–MEK1/2–ERK1/2 pathway in osteosarcoma

Chen, Lei; Wang, Qing; Wang, Guodong; Wang, Huasong; Huang, Yong; Liu, Ximing; Cai, Xianhua

doi:10.1016/j.febslet.2013.03.007

Cited by 102 publications

(85 citation statements)

References 24 publications

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“…In osteosarcoma, specific miRNA expression has been analyzed in tumor cell lines as well as in primary human samples and fixed specimens [10,11,12]. Multiple miRNAs including miR-199a-3p, miR-221, miR-34c and miR-16 have been implicated in the development of osteosarcoma, which function as tumor suppressors or oncogenes [13,14,15,16]. …”

Section: Introductionmentioning

confidence: 99%

MiR-142-3p Functions as a Potential Tumor Suppressor in Human Osteosarcoma by Targeting HMGA1

Wang

Jia

et al. 2014

Cell Physiol Biochem

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Background/Aims: Mounting evidence has shown that aberrant expression of miRNAs correlates with human cancers, and that miRNAs can function as tumor suppressors or oncogenes. Here, we investigated the role and mechanism of miR-142-3p in human osteosarcoma. Methods: We used quantitative real-time RT-PCR to measure the expression of miR-142-3p in human osteosarcoma cell lines and tissues. The roles of miR-142-3p in osteosarcoma development were studied using cultured HOS, MG63 and Saos-2 cells and tumor xenograft analyses in nude mice; their target genes were also investigated. Results: We found that miR-142-3p was significantly downregulated in osteosarcoma cell lines and clinical specimens. Overexpression of miR-142-3p suppressed osteosarcoma cell proliferation, migration and invasion, whereas miR-142-3p knockdown increased these parameters. The xenograft mouse model also revealed the suppressive effect of miR-142-3p on tumor growth. High mobility group AT-hook 1 (HMGA1) was identified as a target of miR-142-3p. Downregulation of HMGA1 induced effects on osteosarcoma cell lines similar to those induced by miR-142-3p. In contrast, restoration of HMGA1 abrogated the effects induced by miR-142-3p up-regulation. Conclusion: These results indicated that miR-142-3p may function as a tumor suppressor by targeting HMGA1 in osteosarcoma.

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Section: Introductionmentioning

confidence: 99%

MiR-142-3p Functions as a Potential Tumor Suppressor in Human Osteosarcoma by Targeting HMGA1

Wang

Jia

et al. 2014

Cell Physiol Biochem

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“…Previous studies have shown that multiple miRNAs are differentially expressed in human cancers, including osteosarcoma [6,7,8], providing new insights into the complex genetic mechanisms of cancer progression. For instance, miR-16 was shown to inhibit cell proliferation by targeting IGF1R and the Raf1-MEK1/2-ERK1/2 pathway in osteosarcoma [9], while miR-802 promoted osteosarcoma cell proliferation by down-regulation of cell-cycle inhibitor, p27 [10]. …”

Section: Introductionmentioning

confidence: 99%

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

Luο

Tang

Gao

et al. 2014

Cell Physiol Biochem

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Background: Multiple MicroRNAs (miRNAs) have been identified in the development and progression of osteosarcoma. However, the expression and roles of miR-212 in osteosarcoma remain largely undefined. Methods: Real-time PCR assays were used to detect the expression of miR-212 in human osteosarcoma tissues. MiR-212 mimics were introduced into MG63 and U2OS cells. Bioinformatic prediction was used to identify the potential targets of miR-212. Protein expression analysis, luciferase assays and rescue assays were used to confirm the substrate of miR-212. Results: miR-212 was significantly down-regulated in human osteosarcoma tissues, compared with adjacent normal tissues. Introduction of miR-212 mimics into MG63 and U2OS cells inhibited cell proliferation and invasion. Besides, miR-212 overexpression could also inhibit tumor growth in the nude mice. Additionally, bioinformatic prediction suggested that the sex-determining region Y-box 4 (Sox4) is a target gene of miR-212. Sox4 inhibition phenocopied the roles of miR-212, while restored expression of Sox4 dampened miR-212-mediated suppression of tumor progression. Conclusion: The miR-212/Sox4 interaction plays an important role of in the osteosarcoma progression.

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“…Multiple members of the miR-16 family were decreased in OS tumor samples, and lower levels of one family member associated with chemoresistance [ 7 ]. These two groups of oncogenic and tumor suppressive miRs have been identifi ed by other groups as well [ 8 ]. For example, the well-known MG-63 OS cell line was found to overexpress miR-181a [ 9 ].…”

Section: Profi Les and Patterns Of Micrornas In Osteosarcomamentioning

confidence: 97%

MicroRNAs in Osteosarcomagenesis

Kafchinski

Jones

2014

Advances in Experimental Medicine and Biology

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The etiology of osteosarcoma (OS) remains enigmatic. Particular clinical and molecular patterns, observed with high frequency in OS, suggest that it results from some yet-to-be-discovered central driver. How else can biology generate such an aggressive, metastatic, genetically and chromosomally unstable malignancy with virtually no apparent precursor neoplasms that are partway along a disease path toward OS? With this conundrum as a backdrop, the discovery of every new native molecule with power to impact a cell's biology is usually quickly followed by a search to see if this type of molecule contains the key to unlock OS biology.

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miR‐16 inhibits cell proliferation by targeting IGF1R and the Raf1–MEK1/2–ERK1/2 pathway in osteosarcoma

Cited by 102 publications

References 24 publications

MiR-142-3p Functions as a Potential Tumor Suppressor in Human Osteosarcoma by Targeting HMGA1

MiR-142-3p Functions as a Potential Tumor Suppressor in Human Osteosarcoma by Targeting HMGA1

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

MicroRNAs in Osteosarcomagenesis

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