miR-182 inhibits Schwann cell proliferation and migration by targeting FGF9 and NTM, respectively at an early stage following sciatic nerve injury

Abstract

The regulation of Schwann cell (SC) responses to injury stimuli by microRNAs (miRNAs) remains to be explored. Here, we identified 17 miRNAs that showed dynamic expression alterations at five early time points following rat sciatic nerve resection. Then we analyzed the expression pattern of 17 miRNAs, and integrated their putative targets with differentially expressed mRNAs. The resulting 222 potential targets were mainly involved in cell phenotype modulation, including immune response, cell death and cell locomotion. Among 17 miRNAs, miR-182 expression was up-regulated. The enhanced expression of miR-182 was correlated with nerve injury-induced phenotype modulation of SCs. Further investigation revealed that fibroblast growth factor 9 (FGF9) and neurotrimin (NTM) were two direct targets of miR-182 in SCs, with miR-182 binding to the 3′-untranslated region of FGF9 and NTM. Silencing of FGF9 and NTM recapitulated the inhibiting effect of miR-182 mimics on SC proliferation and migration, respectively, whereas enforced knockdown of FGF9 and NTM reversed the promoting effect of miR-182 inhibitor on SC proliferation and migration, respectively. Our data indicate that nerve injury inhibits SC proliferation and migration through rapid regulation of miR-182 by targeting FGF9 and NTM, providing novel insights into the roles of miRNAs in nerve injury and repair.

MicroRNA-351 inhibits denervation-induced muscle atrophy by targeting TRAF6

“…All animal experiments were carried out in accordance with the institutional animal care guidelines of Nantong University and approved by the Administration Committee of Experimental Animals, Jiangsu Province. A total of 72 rats underwent surgery for sciatic nerve transection as described previously (21). The TA muscle was harvested from a cohort of rats at 0, 3, 7 and 14 days postsurgery.…”

HMGB1 Protein Does Not Mediate the Inflammatory Response in Spontaneous Spinal Cord Regeneration

“…Cell Migration Assay-Migration of oligodendrocyte Gsn3 was studied using 6.5-mm Transwell chambers with 8-m pores (Corning Costar) as described previously (50). 100 l of oligodendrocytes (2 ϫ 10 5 cells/ml) resuspended in DMEM/ F-12 were transferred to the top chambers of each transwell and allowed to migrate at 30°C in 5% CO 2 for 36 h, and 600 l of DMEM/F-12 including 1 g/ml recombinant proteins or antibodies (1:50) was injected into the lower chambers.…”

Hypoxia-Induced Upregulation of miR-132 Promotes Schwann Cell Migration After Sciatic Nerve Injury by Targeting PRKAG3

“…In the PNS, mature miRNAs are critical for SC differentiation and myelination [9,10]. Our previous studies have also shown that several miRNAs, including miR-221/222, miR-182, and miR-9, are involved in phenotype modulation of SCs after sciatic never injury [11][12][13].…”