miR-186, miR-3651 and miR-494: Potential biomarkers for oral squamous cell carcinoma extracted from whole blood

Ries, Jutta; Vairaktaris, Eleftherios; Agaimy, Abbas; Kintopp, Rita; Baran, Christoph; Neukam, Friedrich Wilhelm; Nkenke, Emeka

doi:10.3892/or.2014.2983

Cited by 76 publications

(61 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Researcher have explored whether circulating miRNAs could be used as potential molecular biomarkers for diseases including human malignancies. Also, studies suggest that differentially expressed miRNA are present in tumor tissues or whole blood of OSCC patients, but few reports described serum miRNAs [9,10]. Thus, we studied 26 differently expressed serum miRNAs from OSCC patients and confirmed that miR-483-5p was significantly correlated with tumor lymph nodal metastases and tumor differentiation.…”

Section: Discussionsupporting

confidence: 86%

Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma

Yang

Zhao

et al. 2015

Tumor Biol.

View full text Add to dashboard Cite

Deregulation of microRNAs (miRNAs) is essential to tumor development, and serum miRNA profiles have been reported in several cancers. However, the serum miRNA profile in oral squamous cell carcinoma (OSCC) remained unclear. The present study aimed to explore abnormal miRNA profile in sera samples from OSCC patients and the association of miR-483-5p with patient prognosis. Microarray analysis was performed in sera from OSCC patients versus healthy controls. miR-483-5p expression was measured by reverse transcription (RT)-PCR and correlated to clinicopathological characteristics of OSCC patients. The prognostic significance was then evaluated with a Kaplan-Meier curve and log-rank tests, using a Cox proportional hazard model. According to microRNA array, 16 miRNAs were upregulated and 10 were downregulated in OSCC patient sera. miR-483-5p expression was significantly increased in OSCC patients (3.23-fold, p < 0.01), and this was significantly correlated with tumor nodal metastasis (TNM) stage and lymph nodal metastases (p < 0.01, p < 0.01). For predicting OSCC, receiver operating characteristic (ROC)/area under the curve (AUC) analysis confirmed a AUC of 0.85 (sensitivity of 0.853 and specificity of 0.746). OSCC patients with high serum miR-483-5p had lower survival than those with low expression, and multivariate analyses for overall survival revealed that high serum miR-483-5p expression was an independent prognostic factor for OSCC (HR = 2.32, 95 %CI 1.20-4.48). miR-483-5p expression increased in OSCC patient sera, and this may be a novel diagnostic and prognostic biomarker for OSCC.

show abstract

Section: Discussionsupporting

confidence: 86%

Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma

Yang

Zhao

et al. 2015

Tumor Biol.

View full text Add to dashboard Cite

show abstract

“…In gastrointestinal stromal tumor cells, lung cancer cells (12), medulloblastoma cells (21), oral cancer cells (22), esophageal squamous cell carcinoma cells (23), gastric cancer (24) and cholangiocarcinoma (21), miR-494 functions as a tumor suppressor gene. By contrast, in glioma cells, oral squamous cell carcinoma (25), bronchial cancer (26) and hepatocellular carcinoma (15), miR-494 functions as an oncogene. In the present study, the expression profile of miR-494 was evaluated in 25 pairs of tumor and adjacent non-tumor samples.…”

Section: Discussionmentioning

confidence: 99%

miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2

et al. 2016

View full text Add to dashboard Cite

Abstract. MicroRNAs (miRs) have been reported to be key regulators in numerous types of cancer. The aim of the present study was to investigate the role of miR-494 in ovarian cancer. Expression of miR-494 was analyzed in ovarian cancer tissues and cell lines by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). miR-494 mimic or negative control was transiently transfected into A2780 and SKOV3 cell lines. A cell counting kit-8 assay was performed to assess the effects of miR-494 on cell proliferation, and flow cytometry was used to evaluate the apoptotic rate. The target gene of miR-494 was detected by luciferase assay. Expression of fibroblast growth factor receptor 2 (FGFR2) was identified using RT-qPCR and western blotting. In the present study, decreased expression of miR-494 was observed in ovarian cancer samples and cell lines. Overexpression of miR-494 inhibited ovarian cancer cell proliferation by inducing apoptosis. Additional investigation indicated that FGFR2 was a direct target of miR-494. Taken together, the results of the present study suggested that miR-494 suppressed ovarian cancer cell proliferation by inducing apoptosis via targeting FGFR2.

show abstract

“…The level of miR-494 was higher in a number of cancers such as classical Hodgkin's lymphoma [4] and oral squamous cell carcinoma [5]. Anti-miR-494 treatment significantly diminished tumor size in mice with primary myc-driven liver tumor [6].…”

Section: Introductionmentioning

confidence: 99%

Tumor-derived microRNA-494 promotes angiogenesis in non-small cell lung cancer

et al. 2015

View full text Add to dashboard Cite

Angiogenesis, a crucial step in tumor growth and metastasis, is regulated by various pro- or anti-angiogenic factors. Recently, microRNAs have been shown to modulate angiogenic processes by modulating the expression of critical angiogenic factors. However, roles of tumor-derived microRNAs in regulating tumor vascularization remain to be elucidated. In this study, we found that delivery of miR-494 into human vascular endothelial cells (ECs) enhanced the EC migration and promoted angiogenesis. The angiogenic effect of miR-494 was mediated by the targeting of PTEN and the subsequent activation of Akt/eNOS pathway. Importantly, co-culture experiments demonstrated that a lung cancer cell line, A549, secreted and delivered miR-494 into ECs via a microvesicle-mediated route. In addition, we found that the expression of miR-494 was induced in the tumor cells in response to hypoxia, likely via a HIF-1α-mediated mechanism. Furthermore, a specific miR-494 antagomiR effectively inhibited angiogenesis and attenuated the growth of tumor xenografts in nude mice. Taken together, these results demonstrated that miR-494 is a novel tumor-derived paracrine signal to promote angiogenesis and tumor growth under hypoxic condition.

show abstract

miR-186, miR-3651 and miR-494: Potential biomarkers for oral squamous cell carcinoma extracted from whole blood

Cited by 76 publications

References 44 publications

Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma

Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma

miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2

Tumor-derived microRNA-494 promotes angiogenesis in non-small cell lung cancer

Contact Info

Product

Resources

About