MiR-193a-5p/ERBB2 act as concurrent chemoradiation therapy response indicator of esophageal squamous cell carcinoma

Lin, Chunru; Tsai, Chen Hsun; Yeh, Ching Tung; Liang, Jin-Wei; Hung, Wan Chun; Lin, Fengyi; Chang, Wei; Li, Hao Yi; Yao, Yun; Hsu, Tai-I; Lee, Yu Cheng; Wang, Yi‐Ching; Sheu, Bor Shyang; Lai, Wu‐Wei; Calkins, Marcus J.; Hsiao, Michael; Lu, Pei Jung

doi:10.18632/oncotarget.9444

Cited by 32 publications

(29 citation statements)

References 31 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Over the past few years, miR-193a-5p has been found to be involved in multiple cancers, such as esophageal squamous cell carcinoma ( 14 ), bladder cancer ( 15 ), primary bone tumors ( 16 ), osteosarcoma ( 17 ), colorectal cancer ( 18 ) and endometrioid endometrial adenocarcinoma ( 19 ). Some noted pathways such as the PI3K/AKT signaling pathway, PTEN/AKT signaling pathway and mTOR signaling pathway have been implicated in the oncogenesis of different tumors and as therapeutic drug targets ( 20 – 24 ).…”

Section: Introductionmentioning

confidence: 99%

A meta‑analysis and bioinformatics exploration of the diagnostic value and molecular mechanism of miR‑193a‑5p in lung cancer

et al. 2018

View full text Add to dashboard Cite

Lung cancer is a leading cause of mortality worldwide and despite recent improvements in lung cancer treatments patient mortality remains high. miR-193a-5p serves a crucial role in the initiation and development of cancer; it is necessary to understand the underlying molecular mechanisms of miR-193a-5p in lung cancer, which may enable the development of improved clinical diagnoses and therapies. The present study investigated the diagnostic value of peripheral blood and tissue miR-193a-5p expression using a microarray meta-analysis. Peripheral blood miR-193a-5p was revealed to be upregulated in patients with lung cancer. The pooled area under the curve (AUC) was 0.67, with a sensitivity and specificity of 0.74 and 0.56, respectively. Conversely, the peripheral tissue miR-193a-5p expression in patients with lung cancer was significantly downregulated. The pooled AUC was 0.83, and the sensitivity and specificity were 0.65 and 0.89, respectively. Through bioinformatics analysis, three Kyoto Encyclopedia of Genes and Genomes (KEGG) terms, pathways in cancer, prostate cancer and RIG-I-like receptor signaling pathway, were identified as associated with miR-193a-5p in lung cancer. In addition, in lung cancer, six key miR-193a-5p target genes, receptor tyrosine-protein kinase erbB-2 (ERBB2), nuclear cap-binding protein subunit 2 (NCBP2), collagen α-1(I) chain (COL1A1), roprotein convertase subtilisin/kexin type 9 (PCSK9), casein kinase II subunit α (CSNK2A1) and nucleolar transcription factor 1 (UBTF), were identified, five of which were significantly upregulated (ERBB2, NCBP2, COL1A1, CSNK2A1 and UBTF). The protein expression of ERBB2, NCBP2, COL1A1, CSNK2A1 and UBTF was also upregulated. NCBP2 and CSNK2A1 were negatively correlated with miR-193a-5p. The results demonstrated that miR-193a-5p exhibited opposite expression patterns in peripheral blood and tissue. Upregulated peripheral blood miR-193a-5p and downregulated tissue miR-193a-5p may be promising diagnostic biomarkers in lung cancer. In addition, the KEGG terms pathways in cancer, prostate cancer and RIG-I-like receptor signaling pathway may suggest which pathways serve vital roles in lung cancer by regulating miR-193a-5p. In addition, six genes, ERBB2, COL1A1, PCSK9, UBTF and particularly NCBP2 and CSNK2A1, may be key target genes of miR-193a-5p in lung cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

A meta‑analysis and bioinformatics exploration of the diagnostic value and molecular mechanism of miR‑193a‑5p in lung cancer

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that the 3'-untranslated region (UTR) of target mRNAs can be bound by specific miRNAs to play important roles in the regulation of radiation sensitivity of ESCC [18,19]. Wnt3 is a member of Wnt family, and is the key molecule of Wnt-β-catenin signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

CircRNA_100367 regulated the radiation sensitivity of esophageal squamous cell carcinomas through miR-217/Wnt3 pathway

Liu

Xue

Guo

et al. 2019

Aging

128

View full text Add to dashboard Cite

Background: Circular RNAs (circRNAs) play important roles in regulating the radioresistance of esophageal squamous cell carcinoma (ESCC). This study aimed to determine the role of hsa_circRNA_100367 in regulating radioresistance of ESCC. Results: Higher expression and potency of endothelial to mesenchymal transformation (EMT) was found in radioresistant ESCC cells (KYSE-150R) than in ESCC cells (KYSE-150). Silencing circRNA_100367 inhibited the proliferation and migration of KYSE-150R cells, and decreased the expression of β-catenin (an important molecule in Wnt pathway) in KYSE-150R cells. Additionally, circRNA_100367 bound to miR-217, and miR-217 targeted Wnt3. Low Wnt3 expression was associated with the short survival time in patients with ESCC and Wnt3 knockdown inhibited the proliferation and migration of KYSE-150R cells. CircRNA_100367 enhanced the radioresistance of KYSE-150R cells through miR-217/Wnt3 pathway. In vivo, circRNA_100367 silence reduced the growth of KYSE-150R cells under radiation. Conclusion: Our results revealed that circRNA_100367 attenuated radioresistance of ESCC through miR-217/Wnt3 pathway. Methods: CircRNAs related with the radioresistance of ESCC were analyzed by hierarchical cluster analysis. The relationship between circRNA_100367 and miR-217, Wnt3 was detected by RNA immunoprecipitation (RIP), RNA pull-down and luciferase reporte assays. The proliferation and migration ESCC cells were detected by MTT, Transwell and colony formation assays.

show abstract

“…For the included 96 studies, 52 were excluded because the frequency of them evaluating prognostic value of miRNA was equal to 1 3 , 4 , 16 , 18 – 23 , 26 , 30 , 31 , 39 – 46 , 48 , 53 , 54 , 56 , 60 , 61 , 64 – 74 , 77 – 86 , 92 – 96 . In addition, although one article reported OS results about miR-193a-5p, it was excluded because it had non-dichotomous miRNA expression value 105 . The mean NOS score of the included researches was 6.5 (4.0–8.0), indicating that the quality of them was adequate (Supplementary Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

Prognostic Value of MicroRNAs in Esophageal Carcinoma: A Meta-Analysis

Gao

Zhao

Zhang

et al. 2018

Clinical and Translational Gastroenterology

View full text Add to dashboard Cite

BackgroundNumerous articles have reported that abnormal expression levels of microRNAs (miRNAs) are related to the survival times of esophageal carcinoma (EC) patients, which contains esophageal adenocarcinoma (EAC) and esophageal squamous cell carcinoma (ESCC). Nevertheless, there has not been a comprehensive meta-analysis to assess the accurate prognostic value of miRNAs in EC.MethodsStudies published in English up to April 12, 2018 that evaluated the correlation of the expression levels of miRNAs with overall survival (OS) in EC were identified by online searches in PubMed, EMBASE, Web of Science, and the Cochrane Database of Systematic Reviews performed by two independent authors. The pooled hazard ratios (HRs) with 95% confidence intervals (CIs) were used to estimate the correlation between OS and miRNA expression. HR ≥ 2 was considered cutoff for considering the miRNA as prognostic candidate.ResultsForty-four pertinent articles with 22 miRNAs and 4310 EC patients were ultimately included. EC patients with tissue expression levels of high miR-21 or low miR-133a (HR = 2.48, 95% CI = 1.50–4.12), miR-133b (HR = 2.15, 95% CI = 1.27–3.62), miR-138 (HR = 2.27, 95% CI = 1.68–3.08), miR-203 (HR = 2.83, 95% CI = 1.35–5.95), miR-375 and miR-655 (HR = 2.66, 95% CI = 1.16–6.12) had significantly poorer OS (P < 0.05). In addition, EC patients with blood expression levels of high miR-21 (HR = 2.19, 95% CI = 1.31–3.68) and miR-223 had significantly shorter OS (P < 0.05).ConclusionsIn conclusion, tissue expression levels of miR-21, miR-133a, miR-133b, miR-138, miR-203, miR-375, and miR-655 and blood expression levels of miR-21 and miR-223 demonstrate significant prognostic value. Among them, the expression levels of miR-133a, miR-133b, miR-138, miR-203, and miR-655 in tissue and the expression level of miR-21 in blood are potential prognostic candidates for predicting OS in EC.

show abstract

MiR-193a-5p/ERBB2 act as concurrent chemoradiation therapy response indicator of esophageal squamous cell carcinoma

Cited by 32 publications

References 31 publications

A meta‑analysis and bioinformatics exploration of the diagnostic value and molecular mechanism of miR‑193a‑5p in lung cancer

A meta‑analysis and bioinformatics exploration of the diagnostic value and molecular mechanism of miR‑193a‑5p in lung cancer

CircRNA_100367 regulated the radiation sensitivity of esophageal squamous cell carcinomas through miR-217/Wnt3 pathway

Prognostic Value of MicroRNAs in Esophageal Carcinoma: A Meta-Analysis

Contact Info

Product

Resources

About