miR-199a-5p induces cell invasion by suppressing E-cadherin expression in cutaneous squamous cell carcinoma

Abstract

A growing quantity of evidence exists to suggest that microRNAs are significant regulators of multiple cellular processes. When expressed aberrantly in different types of cancer, including cutaneous squamous cell carcinoma (cSCC), they play key roles in tumorigenesis and progression. The aberrant expression of miR-199a-5p has been observed to contribute to carcinogenesis in various types of cancer. However, the role of miR-199a-5p in the progression of cSCC metastasis remains largely unknown. In this study, we determined that miR-199a-5p was the upstream regulator of CDH1 (E-cadherin) and that it could suppress the expression of E-cadherin in cSCC cells. In addition, miR-199a-5p mimics significantly induced cell invasion and the activity of matrix metalloproteinase (MMP)2 and MMP9 in cSCC cells. In conclusion, these results are likely to aid in elucidating the molecular mechanisms of cSCC progression. In addition, the findings provide a new theoretical basis to further investigate miR-199a-5p as a potential biomarker and a promising approach in cSCC treatment.

MicroRNA‑186 promotes cell proliferation and inhibits cell apoptosis in cutaneous squamous cell carcinoma by targeting RETREG1

“…Furthermore, the expression of RETREG1 was negatively regulated by miR-186 in CSCC cells, and restoration of RETREG1 attenuated the effects of miR-186 on CSCC cells. In recent decades, a large number of miRs, including, miR-125b (11), miR-365 (20), miR-205 (21), miR-199a-5p (22), and miR-203 (23) have been identified as key regulators of the development and progression of CSCC and numerous other types of human cancer. For instance, miR-199a-5p induces CSCC cell invasion by suppressing E-cadherin expression (22).…”

Knockdown of lncRNA‑UCA1 inhibits the proliferation and migration of melanoma cells through modulating the miR‑28‑5p/HOXB3 axis

“…miRNAs, another type of non-coding small RNA that are 22-25 nucleotides in length, regulate the expression of their target genes through directly binding to the complementary sites in the 3'-UTR region of target mRNAs (25)(26)(27). Similar to lncRNAs, miRs are also associated with various cancers (28)(29)(30)(31)(32). For instance, a previous study demonstrated that miR-33a-5p increased the radiosensitivity of melanoma cells by inhibition of glycolysis (33).…”

“…Given that miRs function through regulating the expression of their target genes (28,29), bioinformatics analysis was conducted to predict the potential target gene of miR-28-5p. We selected HOXB3 for the following studies.…”

MiR‐199a‐5p suppresses tumorigenesis by targeting clathrin heavy chain in hepatocellular carcinoma

“…It is well known that miRNAs function via regulating their target protein by binding to the 3’ untranslated regions (UTRs) of target messenger RNAs (mRNAs), resulting in mRNA degradation or the blockade of mRNA translation . Previously, miR‐199a‐5p was reported to functions as a tumor suppressor by targeting connective tissue growth factor (CTGF), integrin β1, hypoxia‐inducible factor 1 alpha (HIF‐1α), E‐cadherin, hexokinase 2, frizzled class receptor 6 (FZD6), E2F transcription factor 3 (E2F3), Wnt family member 2 (WNT2) and cyclin‐dependent kinase inhibitor 1C (P57, CDKN1C) …”

“…In present study, we showed that miR-199a-5p, frequently down- 19,20 Previously, miR-199a-5p was reported to functions as a tumor suppressor by targeting connective tissue growth factor (CTGF), integrin β1, hypoxia-inducible factor 1 alpha (HIF-1α), E-cadherin, hexokinase 2, frizzled class receptor 6 (FZD6), E2F transcription factor 3 (E2F3), Wnt family member 2 (WNT2) and cyclin-dependent kinase inhibitor 1C (P57, CDKN1C). [21][22][23][24][25][26][27][28][29] Clathrin is considered the prototype vesicle coat protein whose self-assembly mediates sorting of membrane cargo and recruitment of lipid modifiers. 30 In the past decades, most of studies were focused on clathrin mediated endocytosis.…”