MiR-19a Overexpression in FTC-133 Cell Line Induces a More De-Differentiated and Aggressive Phenotype

Calabrese, Giovanna; Dolcimascolo, Anna; Torrisi, Filippo; Zappalà, Agata; Gulino, Rosario; Parenti, Rosalba

doi:10.3390/ijms19123944

Cited by 17 publications

(11 citation statements)

References 78 publications

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“…By regulating target genes of various signalling pathways including gene expression of known oncogenes and tumour suppressor genes, they play a crucial role for cell differentiation, migration, invasion and even epithelial-to-mesenchymal transition (EMT) [26][27][28]. Furthermore, different and distinct miRNA profiles were identified in different TC subtypes [28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Molecular Markers Guiding Thyroid Cancer Management

Nylén

Mechera

Maréchal-Ross

et al. 2020

Cancers

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The incidence of thyroid cancer is rapidly increasing, mostly due to the overdiagnosis and overtreatment of differentiated thyroid cancer (TC). The increasing use of potent preclinical models, high throughput molecular technologies, and gene expression microarrays have provided a deeper understanding of molecular characteristics in cancer. Hence, molecular markers have become a potent tool also in TC management to distinguish benign from malignant lesions, predict aggressive biology, prognosis, recurrence, as well as for identification of novel therapeutic targets. In differentiated TC, molecular markers are mainly used as an adjunct to guide management of indeterminate nodules on fine needle aspiration biopsies. In contrast, in advanced thyroid cancer, molecular markers enable targeted treatments of affected signalling pathways. Identification of the driver mutation of targetable kinases in advanced TC can select treatment with mutation targeted tyrosine kinase inhibitors (TKI) to slow growth and reverse adverse effects of the mutations, when traditional treatments fail. This review will outline the molecular landscape and discuss the impact of molecular markers on diagnosis, surveillance and treatment of differentiated, poorly differentiated and anaplastic follicular TC.

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Section: Introductionmentioning

confidence: 99%

Molecular Markers Guiding Thyroid Cancer Management

Nylén

Mechera

Maréchal-Ross

et al. 2020

Cancers

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“…36 Our group recently demonstrated that miR-19a overexpression in FTC-133 cell line induces a more de-differentiated and aggressive phenotype. 37 In this study, we aim to confirm the role of mir-19a overexpression in thyroid cancer progression and malignancy by using another welldifferentiated cell line, PTC, showing a miR-19a expression level lower than FTC. In addition, to further support the mir-19a function in promoting tumor progression, we evaluated the effect of its inhibition in ATC cell line, which expresses higher basal level than PTC and FTC.…”

Section: Introductionmentioning

confidence: 99%

<p>miR-19a Is Involved In Progression And Malignancy Of Anaplastic Thyroid Cancer Cells</p>

Calabrese¹,

Dolcimascolo²,

Caruso³

et al. 2019

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Background: MicroRNAs (miRNAs) are endogenous, single-stranded, non-coding RNAs acting as negative regulators of gene expression involved in a number of physiological processes. MiRNAs' expression is commonly dysregulated in many types of human tumor diseases and cancers, including thyroid cancers, and is often involved in tumor initiation and progression. miR-19a, a member of miR-17-92 cluster, has been demonstrated to promote cell growth in anaplastic thyroid cancer (ATC), the most advanced and aggressive thyroid cancer. Purpose: In this work, we investigate the potential contribution of miR-19a in thyroid cancer cells poor prognosis and de-differentiation. Methods: We directly modulated the expression of miR-19a in papillary (PTC) and anaplastic thyroid carcinoma cell lines through transfection of specific miR-19a mimic or inhibitor. Further, we performed gene expression analysis of specific genes to evaluate miR-19a association with cell cycle, differentiation, and poor prognosis. Results: Our data indicate that miR-19a overexpression in PTC cells significantly promotes cell growth, decreases the expression of differentiation genes and activates poor prognosis genes. Its inhibition in ATC cells reduces cell proliferation and the expression of genes related to poor prognosis but does not affect differentiation. Conclusion: Our findings reveal the existence of functional associations between miR-19a expression and thyroid cancer progression and malignancy suggesting miR-19a as a novel candidate therapeutic target for ATC.

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“…Some authors also include the possibility of overexpression of microRNAs, which suggests a possible involvement in the differentiation and malignancy [10,11].…”

Section: Introductionmentioning

confidence: 99%

Differentiated Thyroid Carcinoma and Late Onset of Lung Distant Metastasis. A Case Report

Semprebene

Mangano

Ventroni

et al. 2019

Reports

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Background: Differentiated thyroid carcinoma (DTC), which includes the papillary and follicular variants, is a common neoplasm. DTC has a very high cure rate and is treated surgically, usually followed by ablation of the post-surgical remnant with radioiodine. Case Presentation: The case of a 68-year-old male patient who underwent a minimally invasive complete thyroidectomy on July 4, 2007 for capsulated follicular carcinoma with margins of excision exempted from neoplastic infiltration (AJCC 2002 pT2 PNX PMX) is presented. Discussion: As the patient showed the presence of a pulmonary metastasis after 11 years, the potential implications of DTC follow-up management are here summarized. Conclusions: Follow up must be continued throughout life.

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MiR-19a Overexpression in FTC-133 Cell Line Induces a More De-Differentiated and Aggressive Phenotype

Cited by 17 publications

References 78 publications

Molecular Markers Guiding Thyroid Cancer Management

Molecular Markers Guiding Thyroid Cancer Management

<p>miR-19a Is Involved In Progression And Malignancy Of Anaplastic Thyroid Cancer Cells</p>

Differentiated Thyroid Carcinoma and Late Onset of Lung Distant Metastasis. A Case Report

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