miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4

Li, Ming; Li, Jie; Ye, Chaoyang; Wu, Weiwu; C, Yi

doi:10.5114/aoms.2019.89969

Cited by 3 publications

(4 citation statements)

References 34 publications

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“…Decreased STAT4-induced proliferation inhibition and apoptosis in sw780 and T24 cells, however, co-transfection of mir-200a-3p mimics and STAT4 reversed this effect. 36 In contrast, STAT4 showed the tumor suppressor effect in hepatocellular carcinoma (HCC) and was significantly correlated with Ki67 expression, histological grade, HBV infection, and tumor size in HCC clinical cohort. 37 Here, we revealed the prognostic value of STAT4 in clinical cohorts of breast cancer and demonstrated the oncogenic role of STAT4 in breast cancer BT549 cells.…”

Section: Discussionmentioning

confidence: 99%

“…Similar results have been reported in bladder cancer, where the expression of STAT4 in cancerous tissues was higher than that in adjacent non‐cancerous tissues at both the protein and mRNA level. Decreased STAT4‐induced proliferation inhibition and apoptosis in sw780 and T24 cells, however, co‐transfection of mir‐200a‐3p mimics and STAT4 reversed this effect 36 . In contrast, STAT4 showed the tumor suppressor effect in hepatocellular carcinoma (HCC) and was significantly correlated with Ki67 expression, histological grade, HBV infection, and tumor size in HCC clinical cohort 37 …”

Section: Discussionmentioning

confidence: 99%

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STAT4 facilitates PD‐L1 level via IL‐12R/JAK2/STAT3 axis and predicts immunotherapy response in breast cancer

Zhou,

Wan,

Wang

et al. 2023

MedComm

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Signal transducer and activator of transcription 4 (STAT4) is a critical transcription factor for T helper cell differentiation and tumor cells. Although its prognostic role and gene function have been reported in several carcinomas, the role of STAT4 in vitro and in vivo in breast cancer remains poorly understood. The effect of STAT4 in immunotherapy is also unclear. Therefore, we integrated bulk transcriptomics, experiments, and single‐cell transcriptomics to systematically analyze its function in prognosis and signaling pathway. Several clinical breast cancer cohorts confirmed STAT4 as a T‐cell relevant prognostic biomarker. Overexpressed STAT4 increased programmed cell death ligand 1 (PD‐L1) and major histocompatibility complex class II levels in breast cancer cells. In molecular mechanism, transcriptional synergy between STAT4 and STAT3 transactivated interleukin (IL)‐12R and involved a positive feedback loop: STAT4/IL‐12R/JAK2–STAT3–STAT4, which contributed to the upregulation of PD‐L1 expression. The above signaling axis was defined as the STAT4‐related pathway and its score was used to predict T‐cell expansion and anti‐PD1 treatment response. These findings highlight a novel molecular mechanism indirectly regulating PD‐L1 through the STAT4‐related pathway: IL‐12R/JAK2–STAT3–STAT4/PD‐L1, and it has potential application in predicting anti‐PD‐1 immunotherapy response, which may pave the way for stratified immunotherapy in breast cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

STAT4 facilitates PD‐L1 level via IL‐12R/JAK2/STAT3 axis and predicts immunotherapy response in breast cancer

Zhou,

Wan,

Wang

et al. 2023

MedComm

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“…Cell apoptosis and inflammation are the end results of oxidative stress causing fibrosis, cardiac injury and in extreme conditions heart failure [25][26][27]. A number of studies have suggested that miR-200a halts apoptosis in various cancer conditions [28,29]. Also miR-200a has been reported to downregulate mediators of inflammation such as IL-1 and TNF-α and also suppress the levels of HMGB1 [13].…”

Section: Discussionmentioning

confidence: 99%

MiR-200a promotes the survival of cardiac cells and improves cardiac injury in chronic heart failure rats

Wu¹,

Che

2020

Arch Med Sci

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IntroductionmiRNAs play an important role in cardiovascular abnormalities such as heart failure. In the present work we evaluated the role of miR-200a in the condition of chronic heart failure and also the mechanism involved.Material and methodsIn the study 180 subjects, among whom 100 were reported for chronic heart failure and 80 as normal, were included. ELISA and qRT-PCR was done to evaluate levels of HMGB1 and miR-200a in subjects. The cardiac hemodynamics and functioning, oxidative stress and expression of mediators of inflammation were studied in rats with chronic heart failure induced after transfecting them with miR-200a or HMGB1. Luciferase activity was measured to establish any correlation between HMGB1 and miR-200a.ResultsThe chronic heart failure patients included in the study showed suppressed levels of miR-200a and elevated HMGB1 compared to normal subjects. In chronic heart failure rats, the transfection of miR-200a attenuated the cardiac function and other hemodynamic parameters. In addition, improvement in oxidative stress as well as inflammatory mediators was observed. The outcomes also confirmed that HMGB1 was the potential target of miR-200a. It was also noted that upon transfection miR-200a resulted in suppression of protein as well as mRNA levels of HMGB1 in the cardiac tissue of chronic heart failure rats. Also overexpression of HMGB1 decreased the effects of miR-200a.ConclusionsThe outcomes indicate that miR-200a exerts a protective effect on cardiac cell injury via the HMGB1 pathway.

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“…Similarly, other signaling pathways regulating cell proliferation are targeted by miRNAs. MiR-200a binds STAT4 in bladder and liver cancer and decreases cell proliferation in vitro ( 162 , 218 ). In esophageal carcinoma, however, miR-200a acts as oncomiR and targets APC, an inhibitor of β-Catenin/Wnt signaling ( 70 ).…”

Section: Regulation Of Cell Proliferation By Mir-200 Familymentioning

confidence: 99%

The role of miR-200 family in the regulation of hallmarks of cancer

et al. 2022

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MiRNAs are short non-coding RNAs that regulate gene expression post-transcriptionally contributing to the development of different diseases including cancer. The miR-200 family consists of five members, miR-200a, miR-200b, miR-200c, miR-141, and miR-429. Their expression is dysregulated in cancer tissue and their level is altered in the body fluids of cancer patients. Moreover, the levels of miR-200 family members correlate with clinical parameters such as cancer patients’ survival which makes them potentially useful as diagnostic and prognostic biomarkers. MiRNAs can act as either oncomiRs or tumor suppressor miRNAs depending on the target genes and their role in the regulation of key oncogenic signaling pathways. In most types of cancer, the miR-200 family acts as tumor suppressor miRNA and regulates all features of cancer. In this review, we summarized the expression pattern of the miR-200 family in different types of cancer and their potential utility as biomarkers. Moreover, we comprehensively described the role of miR-200 family members in the regulation of all hallmarks of cancer proposed by Hanahan and Weinberg with the focus on the epithelial-mesenchymal transition, invasiveness, and metastasis of tumor cells.

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miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4

Cited by 3 publications

References 34 publications

STAT4 facilitates PD‐L1 level via IL‐12R/JAK2/STAT3 axis and predicts immunotherapy response in breast cancer

STAT4 facilitates PD‐L1 level via IL‐12R/JAK2/STAT3 axis and predicts immunotherapy response in breast cancer

MiR-200a promotes the survival of cardiac cells and improves cardiac injury in chronic heart failure rats

The role of miR-200 family in the regulation of hallmarks of cancer

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