miR-204-5p acts as a tumor suppressor by targeting matrix metalloproteinases-9 and B-cell lymphoma-2 in malignant melanoma

Luan, Wenkang; Yao, Qian; Ni, Xin; Bu, Xuefeng; Xia, Yun; Wang, Jinlong; Ruan, Hongru; Ma, Shaojun; Xu, Bin

doi:10.2147/ott.s128819

Cited by 42 publications

(24 citation statements)

References 33 publications

(42 reference statements)

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“…38 Furthermore, Luan et al reportedmiR-204-5p acts as a tumor suppressor by targeting MMP9 and B-cell lymphoma-2 in malignant melanoma. 22 In the present study, our results showed that miR-204 also inhibited cell proliferation and cell migration by targeting MMP9 in CRC cells. In addition, ectopic PlncRNA-1 could partially rescue suppression of the effect of miR-204 on cell proliferation, cell migration behaviors and EMT process in SW480 cells.…”

Section: Discussionsupporting

confidence: 59%

“…Studies have reported that MMP9 was a direct target of miR‐204 in trophoblast invasion and malignant melanoma . To validate whether miR‐204 targets MMP9 in CRC cells, many experiments were performed.…”

Section: Resultsmentioning

confidence: 99%

“…Studies have reported that MMP9 was a direct target of miR-204 in trophoblast invasion and malignant melanoma. 22,23 To validate whether miR-204 targets MMP9 in CRC cells, many experiments were performed. Consistent with this finding, EGFP reporter assay ( Figures 5A and 5B), RT-qPCR ( Figure 5C) and Western blot analysis ( Figure 5D) showed that MMP9 was negatively regulated by miR-204 in SW480 and SW620 cells.…”

Section: Plncrna-1 Enhanced the Expression Of An Endogenous Mir-204mentioning

confidence: 99%

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Long non‐coding RNA PlncRNA‐1 promotes cell proliferation and hepatic metastasis in colorectal cancer

Ge¹,

Zhang

Wang

et al. 2018

J of Cellular Biochemistry

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Emerging evidence has identified that long non-coding RNAs (lncRNAs) may play an important role in the pathogenesis of many cancer types, including colorectal cancer (CRC). However, the role of PlncRNA-1 in CRC remains unclear. The aim of our present study was to investigate the potential functions of PlncRNA-1 in CRC and to identify the underlying mechanisms of action. We demonstrated that up-regulated PlncRNA-1 in CRC tissues and cells promoted cell proliferation by accelerating cell cycle process and inhibiting cell apoptosis in vitro, enhanced tumor growth and matastasis in vivo and was associated with cell migration and invasion, EMT process of CRC cells. In addition, PlncRNA-1 was a target of miR-204 and enhanced the expression of an endogenous miR-204 target, MMP9 in CRC cells. Furthermore, we found that PlncRNA-1 activates Wnt/β-catenin pathway through the miR-204 in CRC cells. These results suggest that the PlncRNA-1/miR-204/ Wnt/β-catenin regulatory network may shed light on tumorigenesis in CRC.

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Section: Discussionsupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Plncrna-1 Enhanced the Expression Of An Endogenous Mir-204mentioning

confidence: 99%

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Long non‐coding RNA PlncRNA‐1 promotes cell proliferation and hepatic metastasis in colorectal cancer

Ge¹,

Zhang

Wang

et al. 2018

J of Cellular Biochemistry

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“…Abnormally expressed miRNAs play an important role in melanoma formation and progression by serving as potential biomarkers and therapeutic targets (35)(36)(37). In the present study, we investigated the expression levels, the exact roles and the regulatory mechanism of miR-326 in melanoma.…”

Section: Discussionmentioning

confidence: 97%

MicroRNA-326 inhibits melanoma progression by targeting KRAS and suppressing the AKT and ERK signalling pathways

Kang

Zhang

et al. 2017

Oncol Rep

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Abstract. Melanoma is the seventh most common malignancy in females and the fifth most common cancer in males worldwide. An increasing number of studies have reported that microRNA (miRNA) dysregulation is frequently observed in various types of human cancers, including melanoma. Abnormally expressed miRNAs play an important role in melanoma formation and progression by serving as potential biomarkers and therapeutic targets. Recently, miRNA-326 (miR-326) has been reported to be differentially expressed in various types of tissues and play important roles in tumourigenesis and tumour development. However, the expression levels, biological roles and underlying mechanisms of miR-326 in melanoma remain unknown. In the present study, we demonstrated that miR-326 was significantly downregulated in melanoma tissues and cell lines. Functional assays revealed that the enforced expression of miR-326 suppressed melanoma cell proliferation and invasion and increased cell apoptosis in vitro. Using bioinformatic analysis, Kirsten rat sarcoma viral oncogene homolog (KRAS) was predicted as a potential target of miR-326. Luciferase reporter assay confirmed that miR-326 could directly target the 3'-untranslated region of KRAS. In addition, reverse transcription-quantitative polymerase chain reaction and western blot analysis revealed that miR-326 upregulation decreased the KRAS expression in melanoma cells at both the mRNA and protein level. Furthermore, KRAS was upregulated in melanoma tissues and inversely correlated with miR-326 expression. In addition, the KRAS knockdown phenocopied the tumour-suppressing effects of miR-326 overexpression on melanoma cells. The restoration of the expression of KRAS markedly reversed the antitumour effects induced by miR-326 overexpression in melanoma cells. Further experiments indicated that miR-326 inactivated the AKT and ERK signalling pathways in melanoma. Collectively, these results revealed that miR-326 serves as a tumour suppressor in melanoma by targeting KRAS and regulating the AKT and ERK signalling pathways, indicating that miR-326 may be a promising therapeutic target for melanoma patients.

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“…Also, miR‐204‐5p has recently been reported to act as tumour suppressor in melanoma, decreasing cell proliferation, migration and invasion, and promoting cell apoptosis in this cancer (Luan et al., ). The same study identified two direct targets of miR‐204‐5p in MMP‐9 and B‐cell lymphoma‐2 (Bcl‐2) protein.…”

Section: Circulating Cf‐mirnas In Cutaneous Melanoma Stagingmentioning

confidence: 99%

Circulating cell‐free microRNAs in cutaneous melanoma staging and recurrence or survival prognosis

Polini

Carpi

Romanini

et al. 2019

Pigment Cell Melanoma Res

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Cutaneous melanoma is a skin cancer with increasing incidence. Identification of novel clinical biomarkers able to detect the stage of disease and suggest prognosis could improve treatment and outcome for melanoma patients. Cell‐free microRNAs (cf‐miRNAs) are the circulating copies of short non‐coding RNAs involved in gene expression regulation. They are released into the interstitial fluid, are detectable in blood and other body fluids and have interesting features of ideal biomarker candidates. They are stable outside the cell, tissue specific, vary along with cancer development and are sensitive to change in the disease course such as progression or therapeutic response. Moreover, they are accessible by non‐invasive methods or venipuncture. Some articles have reported different cf‐miRNAs with the potential of diagnostic tools for melanoma staging, recurrence and survival prediction. Although some concordance of results is already emerging, differences in analytical methods, normalization strategies and tumour staging still will require further research and standardization prior to clinical usage of cf‐miRNA analysis. This article reviews this literature with the aim of contributing to a shared focusing on these new promising tools for melanoma treatment and care.

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miR-204-5p acts as a tumor suppressor by targeting matrix metalloproteinases-9 and B-cell lymphoma-2 in malignant melanoma

Cited by 42 publications

References 33 publications

Long non‐coding RNA PlncRNA‐1 promotes cell proliferation and hepatic metastasis in colorectal cancer

Long non‐coding RNA PlncRNA‐1 promotes cell proliferation and hepatic metastasis in colorectal cancer

MicroRNA-326 inhibits melanoma progression by targeting KRAS and suppressing the AKT and ERK signalling pathways

Circulating cell‐free microRNAs in cutaneous melanoma staging and recurrence or survival prognosis

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