miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3

Zhu, Endong; Zhang, Juanjuan; Zhou, Jie; Yuan, Hairui; Zhao, Wei; Wang, Baoli

doi:10.1530/jme-17-0183

Cited by 22 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kdm6b, Tgfbr2 and Klf3 have been demonstrated to be the direct targets of miR-20a-5p in our previous studies (21,22). In this study, Tob2 was predicted to be a potential target of miR-20a-5p and the potential pairing between miR-20a-5p and Tob2 is shown in Figure 5A.…”

Section: Tob2 Was a Direct Target Gene Of Mir-20a-5psupporting

confidence: 54%

“…In addition, the miR-20a-5p sponge lentivirus (Sponge LV) were made according to previously published protocol (22). Oil-red O staining showed that the sponge LV infection resulted in fewer differentiated adipocytes and less triglyceride content as compared with control LV (Figures 4A,B).…”

Section: Mir-20a-5p Regulated Adipocyte Differentiation and Lipogenesmentioning

confidence: 99%

“…miR-20a-5p, as a member of the miR-17-92 gene cluster, has critical roles in tumorigenesis and development (19,20). We have recently demonstrated that miR-20a-5p regulates adipogenesis in preadipocytes as well as marrow stromal cells (21,22). However, the mechanism underlying the function of miR-20a-5p in governing adipogenesis, as well as the mechanism how miR-20a-5p is regulated, has not yet been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Novel Regulatory Circuit “C/EBPα/miR-20a-5p/TOB2” Regulates Adipogenesis and Lipogenesis

et al. 2020

Self Cite

View full text Add to dashboard Cite

Recent studies have identified growing importance of microRNAs as key regulators of adipocyte differentiation. We have previously reported that miR-20a-5p is able to induce adipogenesis of established adipogenic cell lines and bone marrow derived mesenchymal stem cells (BMSCs). However, the molecular mechanisms by which miR-20a-5p controls adipogenesis and by which miR-20a-5p expression is regulated need to be further explored. In the current study we found that miR-20a-5p expression was induced during adipocyte differentiation from preadipocyte 3T3-L1 and was increased in epididymal white adipose tissue from either ob/ob mice or high fat diet-induced obese mice. Functional studies identified miR-20a-5p as a positive regulator of adipocyte differentiation and lipogenesis in 3T3-L1 by using either synthetic mimics to supplement miR-20a-5p, or using synthetic inhibitor or sponge lentivirus to inactivate endogenous miR-20a-5p. Luciferase activity assay revealed that TOB2 is a novel target of miR-20a-5p and functional experiment demonstrated its negative regulatory role in adipocyte differentiation. Moreover, Tob2 overexpression significantly attenuated adipocyte formation induced by miR-20a-5p supplementation. In-depth investigation of mechanisms that govern miR-20a-5p expression clarified that C/EBPα transcriptionally activated miR-20a-5p expression via binding to the promoter of miR-20a-5p. Taken together, we conclude that a novel C/EBPα/miR-20a-5p/TOB2 circuit exists and regulates adipogenesis and lipogenesis.

show abstract

Section: Tob2 Was a Direct Target Gene Of Mir-20a-5psupporting

confidence: 54%

Section: Mir-20a-5p Regulated Adipocyte Differentiation and Lipogenesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Regulatory Circuit “C/EBPα/miR-20a-5p/TOB2” Regulates Adipogenesis and Lipogenesis

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…For instance, miR-34a, miR-450a-5p, and miR-28- 5p have been revealed to be involved in regulating the osteoblastic differentiation of mesenchymal stem cells [38,39]. In BMSCs, the adipogenic and osteogenic differentiation can also be regulated by miR-383 [40], miR-20a-5p [41], and miR-20a-5p [41]. Moreover, some miRNAs are reported to be involved in the development of SANFH.…”

Section: Discussionmentioning

confidence: 99%

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

Liu

Lei

2020

J Orthop Surg Res

View full text Add to dashboard Cite

Background: It is reported that miR-596 has a potential diagnostic value for non-traumatic osteonecrosis of the femoral head (NOFH), but its underlying mechanisms in NOFH is unclear. Methods: The expression of miR-596 and Smad3 was detected by western blot and quantitative real-time PCR. The relationship between the two molecules was explored using Dual-Luciferase Reporter Assay. Glucocorticoid (GC)dexamethasone, was used to induce bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation, and the effects of miR-596 on BMSC osteogenic differentiation and proliferation were determined.Results: MiR-596 expression was upregulated, while Smad3 expression was inhibited in the bone marrow samples of patients with steroid-induced osteonecrosis of femoral head (SANFH). Overexpression of miR-596 inhibited the proliferation and osteogenic differentiation of BMSCs induced by GC. Meanwhile, the opposite results were observed in the miR-596 inhibitor group. In addition, Smad3 was a target gene of miR-596, and negatively regulated by miR-596. The promotion effect of the miR-596 inhibitor on BMSC proliferation and osteogenic differentiation was reversed by si-Smad3.Conclusion: MiR-596 can suppress GC-BMSC osteoblastic differentiation and proliferation by regulating Smad3 expression.

show abstract

“…Total RNA was extracted from cells using a total RNA isolation kit (Omega Bio-Tek, Norcross, GA, USA), and 0.5 mg RNA of each sample was reverse-transcribed to cDNA with a Reverse Transcription Kit (Thermo Fisher Scientific, Shanghai, China). Quantitative PCR (qPCR) analyses for the mRNA expression were performed as previously described (27) by using TransStart Green qPCR SuperMix (TransGen Biotech, Beijing, China) on the Light Cycler 96 Real-Time PCR System (Roche Diagnostics, Mannheim, Germany). The relative expression levels of the target genes were calculated using the comparative threshold cycle method (28).…”

Section: Quantitative Rt-pcrmentioning

confidence: 99%

Long noncoding RNA Plnc1 controls adipocyte differentiation by regulating peroxisome proliferator‐activated receptor γ

Zhu

Zhang

et al. 2018

FASEB j.

Self Cite

View full text Add to dashboard Cite

Detailed understanding of molecular mechanisms controlling adipogenesis is of great importance to identify new targets for treating obesity. Emerging evidence suggests that long noncoding RNAs (lncRNAs) may play a pivotal role in adipogenesis. Here, we have identified a novel lncRNA, Plnc1, which is transcribed from a position ~25,000 bp upstream of the peroxisome proliferator‐activated receptor γ2(PPAR‐γ2) gene. Plnc1 is abundantly expressed in adipose tissue, and obese mice have higher Plnc1 expression in adipose tissue than nonobese mice. Plnc1 was induced in established adipogenic lines ST2, 3T3‐L1, and C3H10T1/2 as well as in bone marrow stromal cells (BMSCs) after adipogenic treatment. Plnc1 knockdown blocked differentiation of ST2 cells and BMSCs into mature adipocytes, along with the reduction of PPAR‐γ, CCAAT/enhancer binding protein‐α, and adipocyte protein 2. Conversely, overexpression of Plnc1 promoted ST2 cells and BMSCs to fully differentiate. Mechanism studies revealed that Plnc1 could reduce the methylation level of CpG region in the PPAR‐γ2 promoter and enhance the transcriptional activity of the promoter and thereby increase PPAR‐γ2 transcription. Our study suggests that Plnc1 promotes adipogenic differentiation through controlling the key adipogenic transcription factor PPAR‐g and highlights the potential of Plnc1 as a target for new therapies to control metabolic disorders like obesity.—Zhu, E., Zhang, J., Li, Y., Yuan, H., Zhou, J., Wang, B. Long noncoding RNA Plnc1 controls adipocyte differentiation by regulating peroxisome proliferator‐activated receptor γ. FASEB J. 33, 2396–2408 (2019). http://www.fasebj.org

show abstract

miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3

Cited by 22 publications

References 37 publications

A Novel Regulatory Circuit “C/EBPα/miR-20a-5p/TOB2” Regulates Adipogenesis and Lipogenesis

A Novel Regulatory Circuit “C/EBPα/miR-20a-5p/TOB2” Regulates Adipogenesis and Lipogenesis

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

Long noncoding RNA Plnc1 controls adipocyte differentiation by regulating peroxisome proliferator‐activated receptor γ

Contact Info

Product

Resources

About