miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells

Yan, Liang; Ma, Jinzhu; Wang, Yi; Zan, Jiawei; Wang, Zhen; Zhu, Yu; Zhu, Yiping; Ling, Liefeng; Cao, Long; Liu, Xin; Li, Shu; Xu, Lei; Zhou, Qi; Nie, Liuwang; Zhang, Yao

doi:10.3892/etm.2018.6752

Cited by 21 publications

(20 citation statements)

References 38 publications

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“…Also, it was reported that miR-21 targets transforming growth factor b-induced gene (TGFB1), which encodes the same protein with recognized functions in cell proliferation. Specifically, in A549 cells TGFB1 overexpression reduces cell proliferation and overexpression of miR-21 decreases TGFB1 levels, thus promoting aberrant cell proliferation and highlighting the function of miR-21 as an oncomiR in NSCLC (75). Also, there is solid evidence that miR-21 regulates radiosensitivity in lung cancer cells, and the mechanism reported by Jiang et al indicate the interference with PI3K/AKT/mTOR signaling by inhibiting the transcription of pathway programmed cell death 4 (PDCD4) gene (76) (Figure 6).…”

Section: Discussionmentioning

confidence: 97%

The Clinical Utility of miR-21 and let-7 in Non-small Cell Lung Cancer (NSCLC). A Systematic Review and Meta-Analysis

et al. 2020

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Non-small cell lung cancer (NSCLC) remains a problem worldwide due to its rapid progression and low rate of response to treatment. The heterogeneity of these tumors observed in histopathology exam but also in the mutational status and gene expression pattern makes this malignancy difficult to treat in clinic. The present study investigated the effect of miR-21 and let-7 family members as prognostic biomarkers in NSCLC patients based on the results published in different studies regarding this subject until March 2019. The analysis revealed that these two transcripts are steady biomarkers for prediction of patient outcome or survival. Upregulated expression of miR-21 is associated with poor outcome of patients with NSCLC [HR = 1.87, 95% CI = (1.41, 2.47), p < 0.001]. The analysis regarding let-7 family, specifically let-7a/b/e/f, revealed that downregulated expression of these transcripts predicts poor outcome for NSCLC patients [HR = 2.61, 95% CI = (1.58, 4.30), p < 0.001]. Besides, the reliability of these microRNAs is reflected in the fact that their prognostic significance is constant given the different sample types (tissue, FFPE tissue, serum, serum/plasma or exosomes) used in the selected studies.

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Section: Discussionmentioning

confidence: 97%

The Clinical Utility of miR-21 and let-7 in Non-small Cell Lung Cancer (NSCLC). A Systematic Review and Meta-Analysis

et al. 2020

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“…Previous studies have shown that miR-21-5p is significantly increased in NSCLC cell lines and tissues (20)(21)(22); this is positively associated with tumor size, metastasis and poor prognosis of patients with NSCLC (23,24), indicating the oncogenic properties of miR-21-5p. miR-21-5p has been shown to promote NSCLC cell proliferation in vitro (23,25). However, the involvement and mechanisms of miR-21-5p in NSCLC metastasis have yet to be fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA MEG3 inhibits cell migration and invasion of non‑small cell lung cancer cells by regulating the miR‑21‑5p/PTEN axis

et al. 2021

Mol Med Rep

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“…The FASN-3'-UTRluciferase assay was successfully performed to validate the ability of miR-150-5p to target FASN in the mice experiment 52 . MiR-21 was able to induce the tumor growth by targeting TGFBI in non-small cell lung cancer cells 53 and pancreatic cancer cells 54 , demonstrating the relative stability of our predictions though experimental investigation in BLCA is demanded.…”

Section: Discussionmentioning

confidence: 68%

Construction of An Immune-Related CeRNA Network as a Prognostic Signature in Bladder Cancer

Zhou¹,

Tian²,

Yang³

et al. 2020

Preprint

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Background: Immunotherapy has been proved to be effective for bladder cancer (BLCA). However, the molecular network involved in BLCA tumor immune response remains unclear. This study aims to construct an immune-related ceRNA network and to identify the prognostic value. Methods: Based on The Cancer Genome Atlas (TCGA), we used single-sample gene set enrichment analysis (ssGSEA), weighted gene co-expression network analysis (WGCNA) to determine immune-related mRNA, lncRNA and miRNA. Then least absolute shrinkage, and selection operator (LASSO) and Cox regression were performed to identify the mRNAs with high prognostic value, and accordingly, the risk score was calculated. Internal and external validation were performed both in TCGA and GSE13507 with Kaplan-Meier (KM) survival and Receiver Operating Characteristic (ROC) curve analysis. Using the immune-related mRNA, lncRNA and miRNA, a ceRNA network was established via MiRcode, starBase, miRDB, miRTarBase and TargetScan. Besides, we also explore the relationship between the risk score and immune cell infiltration via CIBERSORT algorithm. Results: 5 mRNAs (PCGF3, FASN, DPYSL2, TGFBI and NTF3) were ultimately identified, and KM survival analysis displayed the 5-mRNA risk signature could predict the prognosis of BLCA with high efficacy both in TCGA (p = 1.006e-13) and GSE13507 (p = 7.759e-04). Using miRNA targeting molecular prediction database, an immune-related ceRNA network, including 5 mRNAs, 24 miRNAs and 86 lncRNAs, was constructed. Memory B cells, activated dendritic cells, and regulatory T cells infiltration into tumors were negatively correlated with risk score, while the infiltration levels of macrophages M0, M1 and M2 were positively correlated with risk score. Conclusion: This study helped to better understand the molecular mechanisms of tumor immune response from the view of ceRNA hypothesis, and provided a novel prognostic signature for bladder cancer.

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miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells

Cited by 21 publications

References 38 publications

The Clinical Utility of miR-21 and let-7 in Non-small Cell Lung Cancer (NSCLC). A Systematic Review and Meta-Analysis

The Clinical Utility of miR-21 and let-7 in Non-small Cell Lung Cancer (NSCLC). A Systematic Review and Meta-Analysis

Long non‑coding RNA MEG3 inhibits cell migration and invasion of non‑small cell lung cancer cells by regulating the miR‑21‑5p/PTEN axis

Construction of An Immune-Related CeRNA Network as a Prognostic Signature in Bladder Cancer

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