MiR-21 is Overexpressed in Response to High Glucose and Protects Endothelial Cells from Apoptosis

Zeng, Jie; Xiong, Y; Li, G; Liu, M; He, Tao; Tang, Yun; Y, Chen; Cai, Liquan; Rong-jian, Jiang; Tao, Jun

doi:10.1055/s-0033-1345169

Cited by 43 publications

(31 citation statements)

References 19 publications

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“…At least two microRNAs that can inhibit S100A4 have been reported: miR‐21 in endothelial cells and miR‐187‐3p in hepatocellular carcinoma . Although these studies used other cellular models, these signals may share phenomena with our miR‐124 /S100A4 in VSMCs to reduce cell survival, suggesting that these microRNAs might also play a key role in neointimal proliferation.…”

Section: Discussionmentioning

confidence: 99%

The microRNA miR‐124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium‐binding protein A4 (S100A4)

et al. 2017

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S100 calcium-binding protein A4 (S100A4) induces proliferation and migration of vascular smooth muscle cells (VSMCs). We aimed to find the microRNA regulating S100A4 expression. S100A4 transcripts are abruptly increased in the acute phase of carotid arterial injury 1 day later (at day 1) but gradually decreases at days 7 and 14. Bioinformatics analysis reveals that miR-124 targets S100A4. VSMC survival is attenuated by miR-124 mimic but increased by miR-124 inhibitor. miR-124 decreases immediately after carotid arterial injury but dramatically increases at days 7 and 14. miR-124 inhibitor-induced cell proliferation is blocked by S100A4 siRNA, whereas miR-124-induced cell death is recovered by S100A4. Our findings suggest that miR-124 is a novel regulator of VSMC proliferation and may play a role in the development of neointimal proliferation.

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Section: Discussionmentioning

confidence: 99%

The microRNA miR‐124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium‐binding protein A4 (S100A4)

et al. 2017

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“…For example, Let-7g was reported to target caspase-3 and inhibit the apoptosis induced by oxidized low density lipoprotein (OX-LDL) in endothelial cells [83]. MiRNA-29b promotes high-fat diet-stimulated endothelial permeability and apoptosis in apoE knock-out mice by down-regulating MT1 expression [84], miR-21 is overexpressed in response to high glucose and protects endothelial cells from apoptosis by targeting death-domain associated protein [85]. …”

Section: Discussionmentioning

confidence: 99%

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

Zhang

Liu

et al. 2017

PLoS ONE

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Increasing evidence has shown that miRNAs play important roles in endometrium development during the menstrual cycle in humans and many other animals. Our previous data indicated that miR-182 levels increase 15.55-fold and pleiotrophin (PTN) levels decrease 20.97-fold in the receptive endometrium (RE, D15) compared with the pre-receptive endometrium (PE, D5) in dairy goats. The present study shows that miR-182 is widely expressed in different tissues of dairy goats and that its expression levels are regulated by E2 and P4 in endometrial epithelium cells (EECs). We confirmed that PTN is a target of miR-182 and that miR-182 regulates the protein levels of AKT, Bcl-2, FAS, MAPK, Caspase-3 and SP1 in EECs. Furthermore, miR-182 up-regulates or maintains the expression levels of osteopontin (OPN), cyclooxygenase-2 (COX-2) and prolactin receptor (PRLR) in EECs, suggesting that miR-182 is an important regulatory factor in the construction of endometrial receptivity in dairy goats. In conclusion, miR-182 participates in the development of endometrial receptivity by down-regulating PTN and affecting the expression of select apoptosis-related genes and increasing or maintaining the expression levels of OPN, COX-2 and PRLR in the EECs of dairy goats.

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“…In order to investigate the potential mechanism of miRNAs in the pathogenesis of PDR, we searched the target genes associated with miR-21, miR-181c and miR-1179. We found that miR-21 was reported inducing tumor angiogenesis through targeting PTEN, leading to activate AKT and ERK1/2 signaling pathways, and thereby enhancing HIF-1α and VEGF expression [22], furthermore, miR-21 protected endothelial cell against high glucose-induced endothelial cytotoxicity [23]. These indicated that miR-21 was highly associated with the angiogenesis with the microenvironment of high glucose.…”

Section: Discussionmentioning

confidence: 99%

Serum MiRNA Biomarkers serve as a Fingerprint for Proliferative Diabetic Retinopathy

Shao

Yuan

Chen

et al. 2014

Cell Physiol Biochem

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Background: Diabetic retinopathy (DR) is a retinopathy resulting from diabetes mellitus (DM) which was classified into non-proliferative DR (NPDR) and proliferative DR (PDR). Without an early screening and effective diagnosis, patients with PDR will develop serious complications. Therefore, we sought to identify special serum microRNAs (miRNAs) that can serve as a novel non-invasive screening signature of PDR and test its specificity and sensitivity in the early diagnosis of PDR. Methods: In total, we obtained serum samples from 90 PDR cases, 90 matched NPDR patients and 20 controls. An initial screening of miRNA expression was performed through TaqMan Low Density Array (TLDA). The candidate miRNAs were validated by individual reverse transcription quantitative real-time PCR (RT-qPCR) arranged in an initial and a two-stage validation sets. Moreover, additional double-blind testing was performed in 20 patients clinically suspected of having DR to evaluate the diagnostic value and accuracy of the serum miRNA profiling system in predicting PDR. Results: Three miRNAs were significantly increased in patients with PDR compared with NPDR after the multiple stages. The areas under the receiver operating characteristic (ROC) curves of the validated three-serum miRNAs signature were 0.830, 0.803 and 0.873 in the initial and two validation sets, respectively. Combination of miR-21, miR-181c, and miR-1179 possessed a moderate ability to discrimination between PDR and NPDR with an area under ROC value of 0.89. The accuracy rate of the three-miRNA profile as PDR signature was 82.6%. Conclusions: These data provide evidence that serum miRNAs have the potential to be sensitive, cost-effective biomarkers for the early detection of PDR. These biomarkers could serve as a dynamic monitoring factor for detecting the progression of PDR from NPDR.

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MiR-21 is Overexpressed in Response to High Glucose and Protects Endothelial Cells from Apoptosis

Cited by 43 publications

References 19 publications

The microRNA miR‐124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium‐binding protein A4 (S100A4)

The microRNA miR‐124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium‐binding protein A4 (S100A4)

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

Serum MiRNA Biomarkers serve as a Fingerprint for Proliferative Diabetic Retinopathy

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