MiR‐21 is up‐regulated in psoriasis and suppresses T cell apoptosis

Meisgen, Florian; Xu, Ning; Wei, Tianling; Janson, Peter; Obad, Susanna; Broom, Oliver; Nagy, Nikoletta; Kauppinen, Sakari; Kemény, Lajos; Ståhle, Mona; Pivarcsi, Andor; Sonkoly, Enikö

doi:10.1111/j.1600-0625.2012.01462.x

Cited by 153 publications

(136 citation statements)

References 26 publications

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“…In recent years, with the comprehensive genetic analysis on miR, increasing numbers of specific miR have been discovered to be related with the pathology of psoriasis 19, including miR‐21 20, miR‐146a 21, miR‐424 22 and miR‐99a 23. Other previous studies have also shown that miR‐203, miR‐125b, miR‐99a and miR‐197 were relevant to the pathogenesis of psoriasis by regulating target genes 24, 25, 26, 27.…”

Section: Introductionmentioning

confidence: 99%

Retracted: MicroRNA‐181b negatively regulates the proliferation of human epidermal keratinocytes in psoriasis through targeting TLR4

Feng

Bai

et al. 2016

J Cellular Molecular Medi

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Our study aims to explore the role of microRNA‐181b (miR‐181b) and TLR in the regulation of cell proliferation of human epidermal keratinocytes (HEKs) in psoriasis. Twenty‐eight patients diagnosed with psoriasis vulgaris were selected as a case group with their lesional and non‐lesional skin tissues collected. A control group consisted of 20 patients who underwent plastic surgery with their healthy skin tissues collected. Real‐time quantitative fluorescence polymerase chain reaction (RT‐qPCR), in situ hybridization and immunohistochemistry were used to detect the expressions of miR‐181b and TLR4 in HEKs of healthy skin, psoriatic lesional skin and non‐lesional skin respectively. The 3′ untranslated region (3′UTR) of TLR4 combined with miR‐181b was verified by a dual‐luciferase reporter assay. Western blotting and bromodeoxyuridine were applied for corresponding detection of TLR4 expression and cell mitosis. The expression of miR‐181b in HEKs of psoriatic lesional skin was less than healthy skin and psoriatic non‐lesional skin. In psoriatic lesional and non‐lesional skin, TLR4‐positive cell rates and the number of positive cells per square millimetre were higher than healthy skin. The dual‐luciferase reporter assay verified that miR‐181b targets TLR4. HEKs transfected with miR‐181b mimics had decreased expression of TLR4, along with the decrease of mitotic indexes and Brdu labelling indexes. However, HEKs transfected with miR‐181b inhibitors showed increased TLR4 expression, mitotic indexes and Brdu labelling indexes. HEKs transfected with both miR‐181b inhibitors and siTLR4 had decreased mitotic indexes and Brdu labelling indexes. These results indicate that miR‐181b can negatively regulate the proliferation of HEKs in psoriasis by targeting TLR4.

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Section: Introductionmentioning

confidence: 99%

Retracted: MicroRNA‐181b negatively regulates the proliferation of human epidermal keratinocytes in psoriasis through targeting TLR4

Feng

Bai

et al. 2016

J Cellular Molecular Medi

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“…Alternatively, splenocyte suspensions were generated from MOG (27). Increased miR-21 levels have also been reported in T cells from patients with SLE and psoriasis (28)(29)(30), and these levels correlated with disease activity. Given the ameliorating effect of anti-miR-21 we observed in EAE, the implication of miR-21 in multiple autoimmune disease models, and the expression profile of miR-21 in patients with MS and other autoimmune diseases, silencing miR-21 may be an effective therapeutic approach in the treatment of MS and other Th17-mediated inflammatory diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Increased expression of miR-21 has been observed in human autoimmune conditions including MS, systemic lupus erythematosus (SLE), and psoriasis (27)(28)(29)(30). However, the role of miR-21 and its intrinsic requirement in Th cell differentiation and autoimmunity remains unclear.…”

Section: Mir-21 Promotes Th17 Differentiation Cd4mentioning

confidence: 99%

MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis

et al. 2015

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“…Often a miRNA has hundreds of different target genes, regulating biological functions through the modulation of their expression (9,10). Several miRNAs have been identified to have important and specific functions in the skin, both in keratinocytes (11)(12)(13)(14)(15)(16) and in other cell types (17,18), showing the relevance of this class of molecules in skin biology.…”

Section: Introductionmentioning

confidence: 99%

Activation of Toll‐like receptors alters the microRNA expression profile of keratinocytes

Meisgen

Landén

Bouez

et al. 2014

Experimental Dermatology

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Additional Supporting Information may be found in the online version of this article:Data S1. Method. Figure S1. Expression of AhR and ARNT in the lesional skin of AD and psoriasis patients and in the healthy skin of the control group. Figure S2. Expression of AhR and ARNT in the lesional skin of AD and psoriasis patients and in the healthy skin of the control group. Figure S3. Effect of TCDD and PCB on normal human epidermal keratinocytes. Figure S4. Abstract: Keratinocytes recognize invading pathogens by various receptors, among them Toll-like receptors (TLRs), and provide the first line of defense in skin immunity. The role of microRNAs in this important defense mechanism has not been explored yet. Our aim was to identify microRNAs involved in the innate immune response of keratinocytes. MicroRNA expression profiling revealed that the TLR2 ligand zymosan, the TLR3 ligand poly(I:C) or the TLR5 ligand flagellin significantly altered the microRNA expression in keratinocytes. The regulation of microRNAs was concentration-dependent and it could be neutralized by siRNAs specific for TLR2, TLR3 and TLR5, respectively, confirming the specificity of the TLR response. Interestingly, one microRNA, miR-146a, was strongly induced by all studied TLR ligands, while other microRNAs were regulated in a TLR-or time point-specific manner. These findings suggest an important role for microRNAs in the innate immune response of keratinocytes and provide a basis for further investigations.

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MiR‐21 is up‐regulated in psoriasis and suppresses T cell apoptosis

Cited by 153 publications

References 26 publications

Retracted: MicroRNA‐181b negatively regulates the proliferation of human epidermal keratinocytes in psoriasis through targeting TLR4

Retracted: MicroRNA‐181b negatively regulates the proliferation of human epidermal keratinocytes in psoriasis through targeting TLR4

MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis

Activation of Toll‐like receptors alters the microRNA expression profile of keratinocytes

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