miR-21, miR-221 and miR-150 Are Deregulated in Peripheral Blood of Patients with Colorectal Cancer

Šarlinová, Miroslava; Halasa, Mirko; D, Mistuna; Musak, Ludovit; Iliev, Robert; Slabý, Ondřej; Mazuchova, Jana; Valentová, Vanda; Plank, L; Halašová, Erika

doi:10.21873/anticanres.11124

Cited by 28 publications

(24 citation statements)

References 24 publications

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“…Increased expression of miR-21 and miR-221 was previously found in peripheral blood samples in patients with colorectal cancer (10,13). We also found that miR-221 had higher expression in rectal than in colonic cancer and than in healthy controls.…”

Section: Discussionsupporting

confidence: 79%

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Expression of Circulating miR-155, miR-21, miR-221, miR-30a, miR-34a and miR-29a: Comparison of Colonic and Rectal Cancer

Orosz

Kiss

Gyöngyi

et al. 2018

In Vivo

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Background: Colorectal cancer is an increasing cause of death. Circulating microRNAs (miRs) could be great diagnostic and prognostic biomarkers of colorectal cancer, but further continuation of their utility is needed for their comprehensive application. Materials and Methods: Twentyseven patients with colonic cancer, 16 with rectal cancer and 12 healthy volunteers as controls, were involved in this study. and miR-29a were determined by reverse transcription polymerase chain reaction (RT-PCR) from sera of patients. Results: Expression of miR-155, miR-21 and miR-221 was significantly higher in rectal cancer than in colonic cancer. There was no difference found between those with TNM1 cancer and controls for both cancer types. miR-155, miR-34a and miR-29a were down-regulated in all patients with cancer compared to controls. We did not find any statistically significant up-regulation of miR-221 in patients with colonic cancer compared to controls. In contrast, in patients with rectal cancer, miR-221 expression was higher than in controls. Advanced stage was also linked to higher miR-221 expression compared to early stage. Slight, but statistically significant increase was observed in miR-30a expression in patients with colon cancer compared to control individuals. Conclusion: Our results partly support previous findings. Here we report on differences in the expression of circulating microRNA between colonic and rectal tumours for the first time. Colorectal tumours are becoming more and more significant 1333 This article is freely accessible online.

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Section: Discussionsupporting

confidence: 79%

“…We also found that miR-221 had higher expression in rectal than in colonic cancer and than in healthy controls. Moreover, expression of miR-21 and miR-221 was more pronounced at an advanced tumour stage (11)(12)(13).…”

Section: Discussionmentioning

confidence: 95%

Expression of Circulating miR-155, miR-21, miR-221, miR-30a, miR-34a and miR-29a: Comparison of Colonic and Rectal Cancer

Orosz

Kiss

Gyöngyi

et al. 2018

In Vivo

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show abstract

“…us, there is a compelling need to identify molecular biomarkers for mass screening and early diagnosis of CRC. A report showed that miR-221 (p < 0.0001) was significantly higher in peripheral blood of 71 patients with colorectal cancer in comparison with 80 matched healthy control individuals [107]. Yau and colleagues evaluated the plasma miR-221 level [108] and the stool miR-221 level [109] and found that the plasma miR-221 had a high sensitivity (86%) but poor specificity (41%) and an AUC of only 0.61.…”

Section: Role Of Mir-221/222 In Colorectal Cancermentioning

confidence: 99%

Role of miR-221/222 in Tumor Development and the Underlying Mechanism

Song

Niu

et al. 2019

Journal of Oncology

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MicroRNA-221/222 (miRNA-221/222, miR-221/222) is a noncoding microRNA which is widely distributed in eukaryotic organisms and deeply involved in the posttranscriptional regulation of gene expressions. According to recent studies, abnormal expressions of miR-221/222 are closely related to the occurrence and development of various kinds of malignant tumors. The role of miR-221/222 in tumor development and their potential molecular mechanism in various cancers, including liver cancer, colorectal cancer, cervical cancer, ovarian cancer, and endometrial carcinoma, are summarized and reviewed in this paper. Moreover, the potential translational biomarker role of abnormal miR-221/222 level in tumor or blood circulation for tumor diagnosis is also discussed.

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“…An antecedent study has reported that microRNA-150-5p (miR-150-5p) exacerbates cellular metastasis in non-small cell lung cancer (Li et al 2016). Its role in the progression of colorectal cancer has also been explored (Aherne et al 2015;Sarlinova et al 2016). Deserved to be mentioned, miR-150-5p is sponged by circular RNA ZNF609 (a competitive endogenous RNA) to mediate AKT3 expression in Hirschsprung's disease (Peng et al 2017).…”

Section: Introductionmentioning

confidence: 99%

circCDYL/microRNA-150-5p participates in modulating growth and migration of colon cancer cells

Cui¹,

Dai²,

Ma³

2019

gpb

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Circular RNA-microRNA (circRNA-miR) node has recently been found to modulate cancer process. Here, we investigated whether circCDYL and miR-150-5p exerted biological function in colon cancer cells. Colon cancer tissues were collected and subjected to qRT-PCR assay for circCDYL and miR-150-5p. SW480 and SW620 cells were forced to overexpress circCDYL and miR-150-5p before subjected to viability, colony formation, apoptosis, migration, invasion and protein (associated with proliferation, apoptosis and signaling pathways) assays. To confirm the combined function, the cells were transfected to simultaneously overexpress circCDYL and miR-150-5p. We found circCDYL was generally decreased while miR-150-5p was increased in colon cancer tissues in parallel with the para-carcinoma tissues. In circCDYL-transfected SW480 and SW620 cells, circ-CDYL decreased viability and promoted apoptosis with down-regulation of c-Myc and cyclin D1, up-regulation of p53, and cleavage of caspase-3 and PARP. Besides, migration and invasion behaviors were impeded. By contrast, miR-150-5p showed a carcinogenesis. However, suppressive role of circ-CDYL in cellular growth and migration was restrained in the cells simultaneously transfected with circCDYL and miR-150-5p, which was companied by down-regulation of PTEN and phosphorylation of PI3K, AKT, JAK2 and STAT5. circCDYL overexpression repressed cellular growth and migration via repressing miR-150-5p in colon cancer cells.

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miR-21, miR-221 and miR-150 Are Deregulated in Peripheral Blood of Patients with Colorectal Cancer

Cited by 28 publications

References 24 publications

Expression of Circulating miR-155, miR-21, miR-221, miR-30a, miR-34a and miR-29a: Comparison of Colonic and Rectal Cancer

Expression of Circulating miR-155, miR-21, miR-221, miR-30a, miR-34a and miR-29a: Comparison of Colonic and Rectal Cancer

Role of miR-221/222 in Tumor Development and the Underlying Mechanism

circCDYL/microRNA-150-5p participates in modulating growth and migration of colon cancer cells

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