MiR-210 and miR-155 as potential diagnostic markers for pre-eclampsia pregnancies

Gan, Lu; Liu, Zheng; Wei, Ming Hui; Chen, Yulong; Yang, Xiao-Ning; Chen, Lihong; Xiao, Xiaomin

doi:10.1097/md.0000000000007515

Cited by 57 publications

(34 citation statements)

References 30 publications

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“…All five of the overlapping miRNAs that were higher in PE than control in the Jairajpuri et al 21 data were also consistently higher in the other studies: hsa-miR-650 30 ; hsa-miR-29a 22 , 33 , 34 ; hsa-miR-210 17 , 30 , 32 ; hsa-miR-518b 19 , 25 , 30 ; and hsa-miR-155–5p. 17 Three of the four overlapping miRNAs that were lower in PE than control in the Jairajpuri et al 21 data were also consistently lower in the other studies: hsa-miR-144–3p 22 , 30 , 31 ; hsa-miR-19b1 32 ; and hsa-miR-15b-5p 30 . The fourth overlapping miRNA that was lower in PE than control for the Jairajpuri et al 21 study was also lower in PE in Xu et al 32 but higher in PE in Yang et al 34 It is notable that the C19MC miRNAs were largely seen in the studies that compared PE cases after diagnosis with gestational age-matched non-PE controls.…”

Section: Discussionsupporting

confidence: 58%

“…Starting in 2011, a number of papers have reported on extracellular microRNA (miRNA) biomarkers associated with PE ( Table 1 ). 17 – 35 Importantly, only two previous studies verified their initial findings in an independent cohort. One of these studies collected all samples after diagnosis, with the small discovery cohort (8 cases and 4 controls) being analyzed by small RNA sequencing (RNA-seq) and the verification cohort (38 cases and 32 controls) being analyzed by qRT-PCR.…”

Section: Introductionmentioning

confidence: 83%

“…As mentioned in the Introduction, we found nineteen previous studies reporting on extracellular miRNAs associated with PE, with limited overlap in identified miRNAs among studies ( Table 1 ). 17 – 35 These studies can be divided into twelve discovery studies, which used large qRT-PCR panels, microarrays, or small RNA-seq, 18 , 22 , 23 , 27 – 35 and seven targeted studies, which performed qRT-PCR on small numbers of selected miR-NAs, 17 , 19 – 21 , 24 – 26 most commonly members of the placenta-specific miRNA cluster located at chr19q13 (C19MC). 19 – 21 , 24 , 25 Eleven C19MC miRNAs were identified in four of the discovery studies (the other eight discovery studies did not identify any C19MC miRNAs), 29 , 30 , 33 , 34 but only three specific miRNAs were shared among at least two studies: hsa-miR-517c-3p and hsa-miR-518e-3p were shared between Yang et al 33 and Yang et al, 34 and hsa-miR-519a-3p was common to Yang et al 33 and Timofeeva et al 29 ( Table S1 ).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women

Srinivasan

Treacy

Herrero

et al. 2020

Cell Reports Medicine

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SUMMARY Development of effective prevention and treatment strategies for pre-eclampsia is limited by the lack of accurate methods for identification of at-risk pregnancies. We performed small RNA sequencing (RNA-seq) of maternal serum extracellular RNAs (exRNAs) to discover and verify microRNAs (miRNAs) differentially expressed in patients who later developed pre-eclampsia. Sera collected from 73 pre-eclampsia cases and 139 controls between 17 and 28 weeks gestational age (GA), divided into separate discovery and verification cohorts, are analyzed by small RNA-seq. Discovery and verification of univariate and bivariate miRNA biomarkers reveal that bivariate biomarkers verify at a markedly higher rate than univariate biomarkers. The majority of verified biomarkers contain miR-155–5p, which has been reported to mediate the pre-eclampsia-associated repression of endothelial nitric oxide synthase (eNOS) by tumor necrosis factor alpha (TNF-α). Deconvolution analysis reveals that several verified miRNA biomarkers come from the placenta and are likely carried by placenta-specific extracellular vesicles.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women

Srinivasan

Treacy

Herrero

et al. 2020

Cell Reports Medicine

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“…As a potential diagnostic biomarker, miR-210-3p in the sera of patients with Graves' disease and colorectal cancer has remarkably higher levels than that in the sera of the healthy control groups [ 43 , 44 ]. The expression of miR-210-3p in the sera of pregnant women with preeclampsia was upregulated; hence, some relationship may exist between miR-210-3p and preeclampsia pathogenesis [ 45 ]. OSCC tissues express more miR-210-3p than normal oral mucosa, and this finding is consistent with our results observed in OSCC tissues [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway

Wang

Zhou

et al. 2020

BioMed Research International

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This study is aimed at determining how oral squamous cell carcinoma (OSCC) regulates the angiogenesis of HUVECs through miR-210-3p expression and exploring the relationship among miR-210-3p, its target protein, and the possible mechanism of angiogenesis regulation. miR-210-3p expression was detected in OSCC tissues and juxta cancerous tissues (JCT), and the relationship among miR-210-3p, microvessel density (MVD), and histopathologic features was analyzed. A conditioned medium (CM) of the OSCC cell line CAL27 was collected to stimulate human umbilical vein endothelial cells (HUVECs), and the miR-210-3p levels and tube formation capability of HUVECs were measured. The target protein level of miR-210-3p was altered; then, PI3K/AKT pathway activation in HUVECs was detected. miR-210-3p was tested in exosomes separated from CAL27 CM, and the transfer of miR-210-3p from OSCC exosomes to HUVECs was verified. Then, we found that the OSCC tissues had higher miR-210-3p levels than the JCT, and miR-210-3p level was positively correlated with MVD and tumor grade. CAL27 CM was able to elevate miR-210-3p levels in HUVECs and promoted tube formation. EFNA3 was the target gene of miR-210-3p, and ephrinA3 protein level was able to influence the migration and proliferation of HUVECs. The levels of phosphorylated AKT in the HUVECs increased when ephrinA3 was downregulated, and the upregulation of ephrinA3 resulted in the suppression of the PI3K/AKT pathway. miR-210-3p was detected in exosomes isolated from the CM of CAL27 cells, and miR-210-3p level in the HUVECs was elevated after absorbing the OSCC exosomes. In conclusion, miR-210-3p was more overexpressed in OSCC tissues than in the JCT. The exosomes secreted by OSCC cells were able to upregulate miR-210-3p expression and reduce ephrinA3 expression in HUVECs and promoted tube formation through the PI3K/AKT signaling pathway.

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“…miR-210 has been shown to be upregulated in response to hypoxia-inducible factor (HIF). miR-210 has also been reported to be involved in various pathophysiological pathways such as oxidative stress, cancer, and apoptosis [86]. Liu et al indicated that miR-210 protected renal tubular cells from apoptosis by targeting HIF-1α [60].…”

Section: Versatile Msc-evs-derived Mirnas Modulate Renal Injury and Hmentioning

confidence: 99%

Immunomodulatory and Regenerative Effects of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Renal Diseases

Tsuji

Kitamura

Wada

2020

IJMS

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Mesenchymal stem cells (MSCs) have immunomodulatory and regenerative effects in many organs, including the kidney. Emerging evidence has shown that the trophic effects from MSCs are mainly mediated by the paracrine mechanism rather than the direct differentiation of MSCs into injured tissues. These secretomes from MSCs include cytokines, growth factors, chemokines and extracellular vesicles (EVs) containing microRNAs, mRNAs, and proteins. Many research studies have revealed that secretomes from MSCs have potential to ameliorate renal injury in renal disease models, including acute kidney injury and chronic kidney disease through a variety of mechanisms. These trophic mechanisms include immunomodulatory and regenerative effects. In addition, accumulating evidence has uncovered the specific factors and therapeutic mechanisms in MSC-derived EVs. In this article, we summarize the recent advances of immunomodulatory and regenerative effects of EVs from MSCs, especially focusing on the microRNAs.

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MiR-210 and miR-155 as potential diagnostic markers for pre-eclampsia pregnancies

Cited by 57 publications

References 30 publications

Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women

Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women

OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway

Immunomodulatory and Regenerative Effects of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Renal Diseases

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