miR-218 is downregulated and directly targets SH3GL1 in childhood medulloblastoma

Shi, Jing; Yang, Lu; Wang, Tuanjie; Zhang, Jian; Guo, Xixia; Huo, Xiaoqing; Huang, Niu

doi:10.3892/mmr.2013.1639

Cited by 22 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Indeed, we con rmed that the expression of miR-218 is decreased by 50% in primary tumours and by ~ 70% in recurrent GBM tumours. Decreased miR-218-5p expression levels have also been reported in other types of human cancer, such as medulloblastoma, thyroid cancer and cervical cancer [43][44][45]. We con rmed that the predicted miR-218 targets, the ECM components TN-C and SDC-2, are directly regulated by miR-218.…”

Section: Discussionsupporting

confidence: 67%

miR-218 Affects the ECM Composition and Cell Biomechanical Properties of Glioblastoma Cells

Grabowska

Kuczyński

Piwecka

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundGlioblastoma (GBM) is the most common malignant brain tumour. GBM cells have ability to infiltrate into the surrounding brain tissue, which results in a significant decrease in the patient’s survival rate. Infiltration is a consequence of the low adhesion and high migration of the tumour cells, two features being associated with the highly remodelled extracellular matrix (ECM). MethodsThe expression profile of miRNAs and mRNAs was analysed by qPCR on 19 GBM tissue samples. Than luciferase assay was performed to confirm interaction between miR-218 and target sequences. Next we checked how supplementation of glioma cell line (U118-MG) with microRNA 218 will change expression pattern of TN-C and SDC both on transcript level and on protein level. Analysis of protein level were made with western-blot technique. Last step in expression profiling of genes connected to cellular motility and adhesion was done with use of qPCR analysis after supplementation with miR-218. To assess the abilities of cells to migrate and proliferate real-time cell culture analysis with use of Incelligence system were utilized. Also this results were backed by classic wound-healing assay. To conclude we used atomic force microscopy (AFM) to measure physical properties such as adhesion and stiffness of cells. This study was supported by microscopy analysis of cytoskeleton changes after supplementation of miR-218.ResultsIn this study, we report that ECM composition is partially regulated at the posttranscriptional level by miRNA. Particularly, we show that miR-218, a well-known miRNA suppressor, is involved in direct regulation of ECM components, tenascin-C (TN-C) and syndecan-2 (SDC-2). We demonstrated that the overexpression of miR-218 reduces the mRNA and protein expression levels of TN-C and SDC-2, and subsequently influences biomechanical properties of GBM cells. Atomic force microscopy (AFM) and real-time migration analysis revealed that miR-218 overexpression impairs the migration potential and enhances the adhesive properties of cells. AFM analysis followed by F-actin staining demonstrated that expression level of miR-218 has an impact on cell stiffness and cytoskeletal reorganization. Global gene expression analysis showed deregulation of a number of genes involved in tumour cell motility and adhesion or ECM remodelling upon miR-218 treatment, suggesting further indirect interactions between the cells and ECM. Conclusion The results demonstrated a direct impact of miR-218 reduction in GBM tumours on the qualitative ECM content, leading to changes in the rigidity of the ECM and GBM cells being conducive to increased invasiveness of GBM.

show abstract

Section: Discussionsupporting

confidence: 67%

miR-218 Affects the ECM Composition and Cell Biomechanical Properties of Glioblastoma Cells

Grabowska

Kuczyński

Piwecka

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In agreement with that report, it was determined that miR-218 was expressed at a reduced level in osteosarcoma, compared with osteoblast cells. Previous studies demonstrated that miR-218 may serve a role in tumor suppression through targeting BMI1 (17,23), RET (18), tumor protein D52 (22), KIT (24) and SH3 domain-containing growth factor receptor bound protein 2-like protein 1 (25). Furthermore, it was determined that miR-218 directly targeted the E2F2 3'UTR in osteosarcoma.…”

Section: Discussionmentioning

confidence: 99%

miR‑218 suppresses the proliferation of osteosarcoma through downregulation of E2F2

et al. 2018

View full text Add to dashboard Cite

Osteosarcoma is the most common primary malignant bone tumor type in children and adolescents under 20 years of age. Biological characteristics include invasiveness, metastasis, abnormal differentiation and loss of contact inhibition. microRNAs (miRNAs) are involved in the transcriptional and post-transcriptional regulation of target mRNAs. Previous studies have demonstrated that miR-218 inhibits tumor formation and progression in glioma, colon cancer and renal cell carcinoma; however, the mechanism of action of miR-218 in osteosarcoma has not been completely determined. In the present study, it was demonstrated that miR-218 exhibited low expression and targeted E2F2 in osteosarcoma cells. Additionally, overexpression of miR-218 inhibited osteosarcoma cell proliferation, with the opposite result occurring following the knockdown of miR-218. Furthermore, it was determined that miR-218 inhibited tumor formation and reduced the expression of E2F2 and proliferating cell nuclear antigen in nude mice. Collectively, the present data demonstrated that miR-218 serves an important role in suppressing the proliferation of osteosarcoma cells, potentially regulated by E2F2, which may provide a novel protein marker for the treatment of osteosarcoma.

show abstract

“…LASP1 is up-regulated in bladder [71] and prostate [72] cancer tissues, while SH3GL1 is overexpressed in childhood medulloblastoma [73]. They were both significantly suppressed by miR-218, which inhibited cell migration and invasion in cancer cells.…”

Section: Focal Adhesion Pathwaymentioning

confidence: 98%

MiR-218 Mediates tumorigenesis and metastasis: Perspectives and implications

Zhang

Waye

et al. 2015

Experimental Cell Research

View full text Add to dashboard Cite

miR-218 is downregulated and directly targets SH3GL1 in childhood medulloblastoma

Cited by 22 publications

References 27 publications

miR-218 Affects the ECM Composition and Cell Biomechanical Properties of Glioblastoma Cells

miR-218 Affects the ECM Composition and Cell Biomechanical Properties of Glioblastoma Cells

miR‑218 suppresses the proliferation of osteosarcoma through downregulation of E2F2

MiR-218 Mediates tumorigenesis and metastasis: Perspectives and implications

Contact Info

Product

Resources

About