MiR-22-3p suppresses sepsis-induced acute kidney injury by targeting PTEN

Wang, Xudong; Wang, Yali; Kong, Mingjian; Yang, Jianping

doi:10.1042/bsr20200527

Cited by 49 publications

(31 citation statements)

References 49 publications

(56 reference statements)

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“…Moreover, we found the upregulation of miR-22-3p alleviated airway inflammation in the OVA-induced murine asthma model. In line with our observation, miR-22-3p notably curbed the inflammation and apoptosis by lowering the levels of IL-1β and IL-6 in rats with sepsis-induced acute kidney injury and in HK-2 cells exposed to LPS (Wang et al, 2020b). Therefore, these findings showed that miR-22-3p may participate in the pathogenesis of asthma.…”

Section: Discussionsupporting

confidence: 90%

microRNA‐22‐3p plays a protective role in a murine asthma model through the inhibition of the NLRP3–caspase‐1–IL‐1β axis

Guo

Chen

Zhang

et al. 2021

Experimental Physiology

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Asthma, a great public health burden, is triggered by inflammatory responses in the airways and these are not addressed appropriately by current therapies. This study aims to investigate the regulatory mechanism of microRNA-22-3p (miR-22-3p) on the proliferation of bronchial epithelial cells exposed to lipopolysaccharide (LPS) and expression of pro-inflammatory cytokines in a murine asthma model challenged by ovalbumin. We first confirmed the downregulation of miR-22-3p in the murine asthma model and bronchial epithelial cells. miR-22-3p remarkably reversed the decline in bronchial epithelial cell viability, enhancement in apoptosis rate and release of inflammatory factors induced by LPS. miR-22-3p targeted and conversely regulated NACHT, LRR and PYD domains-containing protein 3 (NLRP3). Overexpression of NLRP3 counteracted the inhibitory effect of miR-22-3p on inflammatory damage in bronchial epithelial cells through activation of caspase-1/interleukin (IL)-1β. In an in vivo model, overexpression of miR-22-3p significantly attenuated airway obstruction and tissue damage in mice. In summary, our study underscores that miR-22-3p serves both as a negative regulator of the NLRP3-caspase-1-IL-1β axis and as a protective factor against the inflammatory response, suggesting a future therapeutic role in asthma. K E Y W O R D Sasthma, caspase-1, IL-1β, microRNA-22-3p, NLRP3 INTRODUCTIONMicroRNAs (miRNAs), short 18-22 nt single-stranded RNAs that play important parts in modulating protein expression and regulating cellular signalling, have been implicated in the development of chronic airway diseases, including asthma (Tan et al., 2020). The symptoms of asthma result from a chronic airway inflammatory response to a trigger, most frequently inhaled allergen(s), that induces airway hyper-

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Section: Discussionsupporting

confidence: 90%

microRNA‐22‐3p plays a protective role in a murine asthma model through the inhibition of the NLRP3–caspase‐1–IL‐1β axis

Guo

Chen

Zhang

et al. 2021

Experimental Physiology

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“…With the rapid development of high-throughput sequencing, a number of miRNAs were identified to be involved in PTEN downregulation. miR-22 has been proved to downregulate PTEN in many different cell types via directly binding to the 3’UTR of PTEN 39 , 40 . In this study, we found MIR22HG overexpression significantly promoted miR-22 expression in hBMSCs.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA MIR22HG promotes osteogenic differentiation of bone marrow mesenchymal stem cells via PTEN/ AKT pathway

et al. 2020

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Osteoporosis is a prevalent metabolic bone disease characterized by low bone mineral density and degenerative disorders of bone tissues. Previous studies showed the abnormal osteogenic differentiation of endogenous bone marrow mesenchymal stem cells (BMSCs) contributes to the development of osteoporosis. However, the underlying mechanisms by which BMSCs undergo osteogenic differentiation remain largely unexplored. Recently, long non-coding RNAs have been discovered to play important roles in regulating BMSC osteogenesis. In this study, we first showed MIR22HG, which has been demonstrated to be involved in the progression of several cancer types, played an important role in regulating BMSC osteogenesis. We found the expression of MIR22HG was significantly decreased in mouse BMSCs from the osteoporotic mice and it was upregulated during the osteogenic differentiation of human BMSCs. Overexpression of MIR22HG in human BMSCs enhanced osteogenic differentiation, whereas MIR22HG knockdown inhibited osteogenic differentiation both in vitro and in vivo. Mechanistically, MIR22HG promoted osteogenic differentiation by downregulating phosphatase and tensin homolog (PTEN) and therefore activating AKT signaling. Moreover, we found MIR22HG overexpression promoted osteoclastogenesis of RAW264.7 cells, which indicated that MIR22HG played a significant role in bone metabolism and could be a therapeutic target for osteoporosis and other bone-related diseases.

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“…The PI3K-Akt pathway is regulated by several miRNAs, such as miR-202-5p [28], miR-101 [29,30], and miR-22-3p [31], which are upregulated in LFF-EVs. It was reported that exosomes promote GCs' proliferation through the P13K-AKT signaling [12] and MAPK signaling pathways [24].…”

Section: Discussionmentioning

confidence: 99%

Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid

Ding

Jin

Kalds

et al. 2021

Animals

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Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and characterized EVs from goat follicular fluid by means of differential ultracentrifugation and Western blotting of marker proteins. Bioinformatics tools were used to detect miRNA expression levels in EVs. Different miRNA expression patterns of EVs exist in small to large follicles. Thirteen differentially expressed miRNAs (seven upregulated and six downregulated) were identified and used for analysis. A total of 1948 predicted target genes of 13 miRNAs were mapped to signaling pathways, and three significantly enriched pathways (FoxO, MAPK, and PI3K-AKT signaling pathways) were involved in follicular development, as revealed by KEGG enrichment analysis. Our findings suggest that EVs in follicular fluid play biofunctional roles during follicular development in goats.

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MiR-22-3p suppresses sepsis-induced acute kidney injury by targeting PTEN

Cited by 49 publications

References 49 publications

microRNA‐22‐3p plays a protective role in a murine asthma model through the inhibition of the NLRP3–caspase‐1–IL‐1β axis

microRNA‐22‐3p plays a protective role in a murine asthma model through the inhibition of the NLRP3–caspase‐1–IL‐1β axis

Long non-coding RNA MIR22HG promotes osteogenic differentiation of bone marrow mesenchymal stem cells via PTEN/ AKT pathway

Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid

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