MiR-23-TrxR1 as a novel molecular axis in skeletal muscle differentiation

Neri, Margherita; Fittipaldi, Simona; Paola, Elisa De; Dimauro, Ivan; Paronetto, Maria Paola; Jackson, Malcolm J.; Caporossi, Daniela

doi:10.1038/s41598-017-07575-0

Cited by 41 publications

(24 citation statements)

References 49 publications

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“…Either loss- and gain-of-function experiments have shown that miR-208b and miR-499 play important roles in muscle fiber identity by activating slow and repressing fast myofiber gene program [ 82 , 83 ]. Several other studies have shown that after 3 hours of one acute exercise miR-1, miR-133a, miR-133-b and miR-181a were all increased [ 10 , 84 , 85 ] whereas, miR-9, miR-23a, miR-23b and miR-31 decreased (Table 2 ). In another study, Nielsen and colleagues (2010) demonstrated that miR-1 and miR-133a expression levels in the vastus lateralis of healthy individuals were significantly upregulated after only one cycle ergometer activity at 65% of maximal power (Pmax).…”

Section: Mirna Functions In Skeletal Muscle Regenerationmentioning

confidence: 95%

Influence of physical exercise on microRNAs in skeletal muscle regeneration, aging and diseases

et al. 2018

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Skeletal muscle is a dynamic tissue with remarkable plasticity and its growth and regeneration are highly organized, with the activation of specific transcription factors, proliferative pathways and cytokines. The decline of skeletal muscle tissue with age, is one of the most important causes of functional loss of independence in older adults. Maintaining skeletal muscle function throughout the lifespan is a prerequisite for good health and independent living.Physical activity represents one of the most effective preventive agents for muscle decay in aging.Several studies have underlined the importance of microRNAs (miRNAs) in the control of myogenesis and of skeletal muscle regeneration and function.In this review, we reported an overview and recent advances about the role of miRNAs expressed in the skeletal muscle, miRNAs regulation by exercise in skeletal muscle, the consequences of different physical exercise training modalities in the skeletal muscle miRNA profile, their regulation under pathological conditions and the role of miRNAs in age-related muscle wasting.Specific miRNAs appear to be involved in response to different types of exercise and therefore to play an important role in muscle fiber identity and myofiber gene expression in adults and elder population.Understanding the roles and regulation of skeletal muscle miRNAs during muscle regeneration may result in new therapeutic approaches in aging or diseases with impaired muscle function or re-growth.

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Section: Mirna Functions In Skeletal Muscle Regenerationmentioning

confidence: 95%

Influence of physical exercise on microRNAs in skeletal muscle regeneration, aging and diseases

et al. 2018

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“…In addition, previous studies have also demonstrated that the expression of miR-23 is dysregulated in angiogenesis, coronary artery disease, immune responses and cancer (15)(16)(17)(18)(19); the dysregulation of miR-23 is associated with numerous of cancer (20)(21)(22). The miR-23 protein family has two members: mir-23a and mir-23b (23). Cai et al (20) demonstrated that the levels of miR-23a were decreased in prostate cancer and that low levels of miR-23a were associated with poor prognosis.…”

Section: Mir-23b-3p Promotes the Apoptosis And Inhibits The Proliferamentioning

confidence: 99%

miR‑23b‑3p promotes the apoptosis and inhibits the proliferation and invasion of osteosarcoma cells by targeting SIX1

et al. 2018

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Osteosarcoma (OS) is the most common primary malignant bone tumor and the third most common cancer that occurs during childhood and adolescence. Increasing evidence has suggested that microRNA (miR)-23b-3p has an important role in OS tumorigenesis; however, the underlying molecular mechanisms remain unknown. The aim of the present study was to investigate the expression levels of miR-23b-3p and sine oculis homeobox homolog 1 (SIX1) in OS tissues and cell lines (MG-63, SaOS-2 and U2OS), as well as to observe the effects of miR-23b-3p on U2OS cell viability, cell cycle, apoptosis and invasive ability. The results revealed that the expression levels of miR-23b-3p were significantly decreased in OS tissues and cell lines compared with tumor-adjacent normal tissues and a non-cancerous human fetal osteoblastic cell line (hFOB1.19). To investigate the underlying mechanisms of miR-23b-3p in OS tumorigenesis and progression, human U2OS cell lines over-or under expressing miR-23b-3p were established. The effects of miR-23b-3p on U2OS cell viability, cell cycle, apoptosis and invasion properties were determined by performing Cell Counting Kit-8, flow cytometry and Transwell invasion assays. miR-23b-3p was revealed to suppress cell viability, proliferation and invasion, and to enhance the levels of cell apoptosis. Furthermore, SIX1 mRNA and protein expression levels in OS tissues and cell lines were significantly upregulated when compared with tumor-adjacent normal tissues and hFOB 1.19 cells, which suggested that SIX1 expression levels may be inversely associated with miR-23b-3p levels in OS. Luciferase reporter system analysis demonstrated that miR-23b-3p binds to the SIX1 3'-untranslated region. miR-23b-3p downregulation contributed to SIX1 upregulation, which facilitated the potentiation of cyclin D1 and vascular endothelial growth factor-C expression levels, as well as the inhibition of caspase-3 expression. Collectively, these results suggested that miR-23b-3p is downregulated and SIX1 is upregulated in OS cells, and that miR-23b-3p inhibition may suppress the proliferation and invasion of OS cells, and contribute to cell apoptosis via negative regulation of SIX1. miR-23b-3p/SIX1 may therefore represent a potential target for the treatment of OS.

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“…However after chronic exercise, miR-1 and miR-133b were downregulated in a majority of studies [50,[55][56][57]. Only two studies reported increased expression of miR-133b and miR-181 which is thought to be associated with increased glucose homeostasis [40,60], and another study found a decrease in miR-23 [51] involved in myogenic processes [61]. A case-control study comparing powerlifters to healthy controls [58] reported a unique miR expression profiles that were able to distinguish powerlifters from healthy controls based on a five miR signature (miR-126, −23b, −16, −23a, −15a).…”

Section: Mirnas and Exercisecandidate Gene Approachmentioning

confidence: 99%

Epigenetic changes in healthy human skeletal muscle following exercise– a systematic review

et al. 2019

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Exercise training is continually challenging whole-body homeostasis, leading to improvements in performance and health. Adaptations to exercise training are complex and are influenced by both environmental and genetic factors. Epigenetic factors regulate gene expression in a tissue-specific manner and constitute a link between the genotype and the environment. Moreover, epigenetic factors are emerging as potential biomarkers that could predict the response to exercise training. This systematic review aimed to identify epigenetic changes that have been reported in skeletal muscle following exercise training in healthy populations. A literature search of five databases (PUBMED, MEDLINE, CINHAL, SCOPUS and SportDiscuss) was conducted in November 2018. Articles were included if they examined epigenetic modifications (DNA methylation, histone modifications and non-coding RNAs) in skeletal muscle, following either an acute bout of exercise, an exercise intervention in a pre/post design, or a case/control type of study. Twenty-two studies met the inclusion criteria. Several epigenetic markers including DNA methylation of genes known to be differentially expressed after exercise and myomiRs were reported to be modified after exercise. Several epigenetic marks were identified to be altered in response to exercise, with potential influence on skeletal muscle metabolism. However, whether these epigenetic marks play a role in the physiological impact of exercise is unclear. Exercise epigenetics is still a very young research field, and it is expected that in the future the causality of such changes will be elucidated via the utilization of emerging experimental models able to target the epigenome.

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MiR-23-TrxR1 as a novel molecular axis in skeletal muscle differentiation

Cited by 41 publications

References 49 publications

Influence of physical exercise on microRNAs in skeletal muscle regeneration, aging and diseases

Influence of physical exercise on microRNAs in skeletal muscle regeneration, aging and diseases

miR‑23b‑3p promotes the apoptosis and inhibits the proliferation and invasion of osteosarcoma cells by targeting SIX1

Epigenetic changes in healthy human skeletal muscle following exercise– a systematic review

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