miR-26 suppresses renal cell cancer via down-regulating coronin-3

Wang, Xin Jun; Yan, Zhi Jian; Luo, Guang; Chen, Yi Yan; Bai, Pei Ming

doi:10.1007/s11010-019-03636-2

Cited by 16 publications

(13 citation statements)

References 15 publications

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“…To explore the underlying mechanism of RASA1 in cells, the effect of RASA1 on the expression of miRNAs closely related to kidney cancer was examined. Here, we assessed miR-132, miR-630, miR-223-3p, miR-508-3p, miR-486, and miR-296-3p [13][14][15][16][17][18][19]. RASA1 overexpression reduced the expression of miR-223-3p ( Figure 5A), but had no notable impacts on the expression of other miRNAs tested (Supplementary Figure S1).…”

Section: Rasa1 Regulates Mir-223-3pmentioning

confidence: 99%

RASA1 inhibits the progression of renal cell carcinoma by decreasing the expression of miR-223-3p and promoting the expression of FBXW7

2020

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Abstract RAS p21 protein activator 1 (RASA1), also known as p120-RasGAP, is a RasGAP protein that functions as a signaling scaffold protein, regulating pivotal signal cascades. However, its biological mechanism in renal cell carcinoma (RCC) remains unknown. In the present study, RASA1, F-box/WD repeat-containing protein 7 (FBXW7), and miR-223-3p expression were assessed via quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot. Then, the targeted correlations of miR-223-3p with FBXW7 and RASA1 were verified via a dual-luciferase reporter gene assay. CCK-8, flow cytometry, and Transwell assays were implemented independently to explore the impact of RASA1 on cell proliferation, apoptosis, migration, and cell cycle progression. Finally, the influence of RASA1 on tumor formation in RCC was assessed in vivo through the analysis of tumor growth in nude mice. Results showed that FBXW7 and RASA1 expression were decreased in RCC tissues and cell lines, while miR-223-3p was expressed at a higher level. Additionally, FBXW7 and RASA1 inhibited cell proliferation but facilitated the population of RCC cells in the G0/G1 phase. Altogether, RASA1 may play a key role in the progression of RCC by decreasing miR-223-3p and subsequently increasing FBXW7 expression.

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Section: Rasa1 Regulates Mir-223-3pmentioning

confidence: 99%

RASA1 inhibits the progression of renal cell carcinoma by decreasing the expression of miR-223-3p and promoting the expression of FBXW7

2020

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“…We previously found that CORO3 serves as tumor-promoting factor to control cell growth and invasion of ccRCC cells. Downregulation of CORO3 by its specific upstream miRNA, miR-26, appeared to prevent CORO3-induced cell growth and invasion (Wang et al, 2020). Other studies have also consistently documented that CORO proteins are overexpressed in various cancers, including breast cancer, gastric cancer, and hepatocellular carcinoma (Thal et al, 2008;Wu et al, 2010).…”

Section: Discussionmentioning

confidence: 88%

CORO6 Promotes Cell Growth and Invasion of Clear Cell Renal Cell Carcinoma via Activation of WNT Signaling

Wang

Xiao

et al. 2021

Front. Cell Dev. Biol.

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Renal cell carcinoma (RCC) constitutes the most lethal type of genitourinary cancer. Understanding of RCC tumor biology helps to identify novel targets and develop directed treatments for patients with this type of cancer. Analysis from both The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma dataset and our RCC samples demonstrated that the expression level of CORO6 was significantly higher in RCC patients than in normal kidney tissues, and its level was highly associated with tumor stage and grade. Importantly, CORO6 expression level was an independent predictor of tumor metastasis and overall survival in RCC patients. Our cell line data also confirmed that CORO6 knockdown could suppress RCC cell growth as well as cell migration and invasion. The depletion of CORO6 led to cell cycle arrest at the G0/G1 phase and caused cell apoptosis. Further, mechanistic dissection showed that CORO6 mediated RCC cell growth, and cell invasion relied on WNT signaling. Moreover, the in vivo data suggested that CORO6 knockdown indeed suppressed RCC tumor growth. Overall, our study defines the oncogenic role of CORO6 in RCC progression and provides a rationale for developing CORO6-targeted therapies for improved treatment of RCC patients.

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“…We previously found that CORO3 serves as tumor-promoting factor to control cell growth and invasion of ccRCC cells. Downregulation of CORO3 by its speci c upstream miRNA, miR-26, appeared to prevent CORO3-induced cell growth and invasion [23]. Other studies have also consistently documented that CORO proteins are overexpressed in various cancers, including breast cancer, gastric cancer, and hepatocellular carcinoma [16,17].…”

Section: Discussionmentioning

confidence: 90%

CORO6 Promotes Cell Growth and Invasion of Clear Cell Renal Cell Carcinoma via Activation of WNT Signaling

Wang

Xiao

Yan

et al. 2020

Preprint

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BackgroundRenal cell carcinoma (RCC) constitutes the most lethal type of genitourinary cancer. Understanding of RCC tumor biology helps to identify novel targets and develop directed treatments for patients with this type of cancer. MethodsBioinformatical Analysis of The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma dataset. Western blotting and qPCR were used to examine the expression levels of interested genes. Flow cytometry, MTT, BrdU staining, wound healing assay and transwell invasion assay were applied to determine the biological functions of CORO6 in ccRCC cells. The in vivo effect of CORO6 on ccRCC development was validated with xenografted mouse model.ResultsAnalysis from both The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma dataset and our RCC samples demonstrated that the expression level of CORO6 was significantly higher in RCC patients than in normal kidney tissues, and its level was highly associated with tumor stage and grade. Importantly, CORO6 expression level was an independent predictor of tumor metastasis and overall survival in RCC patients. Our cell line data also confirmed that CORO6 knockdown could suppress RCC cell growth as well as cell migration and invasion. The depletion of CORO6 led to cell cycle arrest at the G0/G1 phase and caused cell apoptosis. Further, mechanistic dissection showed that CORO6 mediated RCC cell growth, and cell invasion relied on WNT signaling. Moreover, the in vivo data suggested that CORO6 knockdown indeed suppressed RCC tumor growth. ConclusionsOverall, our study defines the oncogenic role of CORO6 in RCC progression and provides a rationale for developing CORO6-targeted therapies for improved treatment of RCC patients.

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miR-26 suppresses renal cell cancer via down-regulating coronin-3

Cited by 16 publications

References 15 publications

RASA1 inhibits the progression of renal cell carcinoma by decreasing the expression of miR-223-3p and promoting the expression of FBXW7

RASA1 inhibits the progression of renal cell carcinoma by decreasing the expression of miR-223-3p and promoting the expression of FBXW7

CORO6 Promotes Cell Growth and Invasion of Clear Cell Renal Cell Carcinoma via Activation of WNT Signaling

CORO6 Promotes Cell Growth and Invasion of Clear Cell Renal Cell Carcinoma via Activation of WNT Signaling

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