miR-26b modulates OA induced BMSC osteogenesis through regulating GSK3β/β-catenin pathway

Hu, Hexiang; Zhao, Chuanlong; Zhang, Peiguang; Liu, Yalong; Jiang, Yulian; Wu, Enquan; Xue, Hao; Liu, Caiyun; Li, Zhehai

doi:10.1016/j.yexmp.2019.02.003

Cited by 36 publications

(26 citation statements)

References 31 publications

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“…Studies for the osteogenesis of BMSCs may provide novel insights to the development of more effective manipulations for treatment of osteoporosis [27] and muscle injuries [26]. In the multidirectional differentiation potential of BMSCs, miRNAs play a role in regulating the differentiation of stem cells in different directions, and the miRNAs involved in regulating the multidirectional differentiation potential of different types of stem cells are also different [28][29][30]. For example, miR-19a-3p promotes the osteogenic differentiation of human-derived mesenchymal stem cells by targeting HDAC4 (PMID: 31248594).…”

Section: Discussionmentioning

confidence: 99%

[Retracted] miRNA‐187‐5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

Sun

Wang

Chen

et al. 2020

BioMed Research International

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Osteoporosis (OP) is a common bone metabolic disease, the process of which is fundamentally irreversible. Therefore, the investigation into osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) will provide more clues for OP treatment. In the present study, we found that microRNA-187-5p (miR-187-5p) played a key role on osteoblastic differentiation, which was significantly upregulated during osteogenic differentiation of BMSCs in mice. Moreover, overexpression of miR-187-5p suppressed osteoblastic differentiation of BMSCs through increasing alkaline phosphatase (ALP), matrix mineralization, and levels of Osterix (OSX), and osteopontin (OPN) as well as runt-related transcription factor 2 (Runx2) in vitro. The results in vivo indicated that the upregulation of miR-187-5p enhanced the efficacy of new bone formation in the heterotopic bone formation assay. Luciferase reporter assay and western blot analysis revealed that miR-187-5p was involved in osteogenesis by targeting intracellular adhesion molecule 1 (ICAM-1). Furthermore, ICAM-1 silence inhibited osteoblastic differentiation of BMSCs. Taken together, our results suggested for the first time that miR-187-5p may promote osteogenesis by targeting ICAM-1, and provided a possible therapeutic target for bone metabolic diseases.

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Section: Discussionmentioning

confidence: 99%

[Retracted] miRNA‐187‐5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

Sun

Wang

Chen

et al. 2020

BioMed Research International

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“…MiR-129-5p targets TCF-4 and its downregulation enhanced osteoblast differentiation in the MC3T3-E1 cell line, and in C57BL6 mice it ameliorated osteoporosis [142]. Further, it has been shown that miR-26a and miR-26b, targeting glycogen synthase kinase three beta (GSK3β), lead to Wnt signaling activation and promote osteogenic differentiation of BM-MSCs [99,143]. Interestingly, miR-26a is increased during osteogenic differentiation induced by PEMFs [128].…”

Section: Transcription Factors: β-Catenin and Tcf/lefmentioning

confidence: 99%

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

Mazziotta

Lanzillotti

Iaquinta

et al. 2021

IJMS

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Mesenchymal stem cells (MSCs) have been identified in many adult tissues and they have been closely studied in recent years, especially in view of their potential use for treating diseases and damaged tissues and organs. MSCs are capable of self-replication and differentiation into osteoblasts and are considered an important source of cells in tissue engineering for bone regeneration. Several epigenetic factors are believed to play a role in the osteogenic differentiation of MSCs, including microRNAs (miRNAs). MiRNAs are small, single-stranded, non-coding RNAs of approximately 22 nucleotides that are able to regulate cell proliferation, differentiation and apoptosis by binding the 3′ untranslated region (3′-UTR) of target mRNAs, which can be subsequently degraded or translationally silenced. MiRNAs control gene expression in osteogenic differentiation by regulating two crucial signaling cascades in osteogenesis: the transforming growth factor-beta (TGF-β)/bone morphogenic protein (BMP) and the Wingless/Int-1(Wnt)/β-catenin signaling pathways. This review provides an overview of the miRNAs involved in osteogenic differentiation and how these miRNAs could regulate the expression of target genes.

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“…Previous studies have proven that decreasing miR-10a-3p and raising CXCL12 can induce the osteogenic differentiation of bone marrow-derived MSCs (BMSCs) [26]. miR-26b could promote BMSCs osteogenesis through directly regulating GSK3β and activating Wnt pathway [27]. MSCs-derived exosomes are involved in multiple physiology and pathology processes, including osteogenesis, bone regeneration and osteoarthritis [28,29].…”

Section: Discussionmentioning

confidence: 99%

LncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived From ADSCs for The Treatment of Osteoporosis

Wang

Jia²,

Chen

2021

Preprint

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Background Osteoporosis is a worldwide medical and socioeconomic threat characterized by systemic impairment to bone strength and microstructure. Exosomes derived from adipose-derived stem cells (ADSCs-Exos) have been confirmed to play effective roles in the repair of various tissues and organs. This study aimed to investigate the role of ADSCs-Exos and a noval long none coding RNAKCNQ1OT1 (lnc-KCNQ1OT1) played in osteoporosis as well as the underlying mechanism. Methods MC3T3-E1 cells were treated with different doses of TNF-α (0, 1, 2.5, 5, 10 ng/ml) and then co-cultured with ADSCs-Exos or exosomes-derived from lnc-KCNQ1OT1-modified ADSCs (KCNQ1OT1-Exos). The expression of microRNA (miRNA)-141-5p (miR-141-5p) and lnc-KCNQ1OT1 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The protein expression of cleaved-caspase-3, caspase-3 and Bax was determined by Western blot. Cell viability and apoptosis were assessed by cell counting kit 8 (CCK-8) and flow cytometry analysis, respectively. The binding sites between KCNQ1OT1 and miR-141-5p were validated by dual-luciferase reporter assay. Results Tumor necrosis factor-α (TNF-α) dose dependently increased miR-141-5p expression, inhibited viability and promoted apoptosis of MC3T3-E1 cells. However, miR-141-5p silencing or co-culture with ADSCs-Exos attenuated these effects. In addition, KCNQ1OT1-Exos could more significantly attenuate the induced cytotoxicity and apoptosis compared to ADSCs-Exos. Moreover, miR-141-5p was confirmed as the target of lnc-KCNQ1OT1 by luciferase reporter assay. Conclusions ADSCs-Exos attenuated cytotoxicity and apoptosis of TNF-α-induced MC3T3-E1 cells. KCNQ1OT1-Exos had a more significant inhibitory effect compared to ADSCs-Exos by the function of sponging miR-141-5p, suggesting that KCNQ1OT1-Exos could be promising agents in osteoporosis treatment.

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miR-26b modulates OA induced BMSC osteogenesis through regulating GSK3β/β-catenin pathway

Cited by 36 publications

References 31 publications

[Retracted] miRNA‐187‐5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

[Retracted] miRNA‐187‐5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

LncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived From ADSCs for The Treatment of Osteoporosis

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