miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways

Wang, Yuhui; Han, Xiaodong; Zang, Tongxin; Kang, Ping; Niu, Niu

doi:10.3892/etm.2020.9045

Cited by 13 publications

(10 citation statements)

References 28 publications

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“…miR-29b may be associated with the regulation of osteogenic and osteolytic differentiation in aseptic inflammation of the bone [ 115 ]. Recent studies found that first miR-29b can promote proliferation and migration of rat bone marrow MSCs through PI3K/AKT and TGF- β /Smad signaling pathways [ 116 ]. These protective effects seen in inflammatory bone diseases may be related to the osteoblast-osteoclast dynamic balance, but further research is needed to elucidate the exact mechanisms.…”

Section: Exosomes and Bone Metabolismmentioning

confidence: 99%

[Retracted] Exosome: Function and Application in Inflammatory Bone Diseases

Wang

Chen

et al. 2021

Oxidative Medicine and Cellular Longevity

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In the skeletal system, inflammation is closely associated with many skeletal disorders, including periprosthetic osteolysis (bone loss around orthopedic implants), osteoporosis, and rheumatoid arthritis. These diseases, referred to as inflammatory bone diseases, are caused by various oxidative stress factors in the body, resulting in long-term chronic inflammatory processes and eventually causing disturbances in bone metabolism, increased osteoclast activity, and decreased osteoblast activity, thereby leading to osteolysis. Inflammatory bone diseases caused by nonbacterial factors include inflammation- and bone resorption-related processes. A growing number of studies show that exosomes play an essential role in developing and progressing inflammatory bone diseases. Mechanistically, exosomes are involved in the onset and progression of inflammatory bone disease and promote inflammatory osteolysis, but specific types of exosomes are also involved in inhibiting this process. Exosomal regulation of the NF-κB signaling pathway affects macrophage polarization and regulates inflammatory responses. The inflammatory response further causes alterations in cytokine and exosome secretion. These signals regulate osteoclast differentiation through the receptor activator of the nuclear factor-kappaB ligand pathway and affect osteoblast activity through the Wnt pathway and the transcription factor Runx2, thereby influencing bone metabolism. Overall, enhanced bone resorption dominates the overall mechanism, and over time, this imbalance leads to chronic osteolysis. Understanding the role of exosomes may provide new perspectives on their influence on bone metabolism in inflammatory bone diseases. At the same time, exosomes have a promising future in diagnosing and treating inflammatory bone disease due to their unique properties.

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Section: Exosomes and Bone Metabolismmentioning

confidence: 99%

[Retracted] Exosome: Function and Application in Inflammatory Bone Diseases

Wang

Chen

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Hence, a clear understanding of the pathogenesis of OP requires a complete dissection and testing of the differentiation process of BMSCs. The differentiation of BMSCs into osteoblasts is regulated by several transcription factors, such as IGF-1, Osterix, Runx2 and others (9)(10)(11)(12). It is essential to understand how these factors regulate the differentiation of BMSCs towards osteoblasts and their function in the development of drugs for the treatment of OP and inflammatory bone diseases.…”

Section: Introductionmentioning

confidence: 99%

Down-regulation of miR-19b-3p enhances IGF-1 expression to induce osteoblast differentiation and improve osteoporosis

Cai

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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This research was conducted in order to investigate the role of miR-19b-3p in the development of osteoporosis (OP) in rats and the associated mechanisms. This study measured the expression levels of miR-19b-3p and IGF-1 in clinical OP patients and ovariectomy-induced OP rats by qRT-PCR. The osteoprotegerin levels in OP patients were measured by enzyme-linked immunosorbent assay (ELISA). The binding site of miR-19b-3p to IGF-1 was predicted by three prediction sites: Target Scan, miRDB and starbase. Experiments were conducted in vitro and in vivo using bone marrow mesenchymal stem cells (BMSCs) and OP rats, respectively, to verify the regulatory relationship between miR-19b-3p and IGF-1 and explore the role of miR-19b-3p in the development of OP. Results showed that the expression of miR-19b-3p was elevated in OP patients and rats, while IGF-1 expression was decreased (***p<0.001). The ELISA assay found that osteoprotegerin levels were inversely correlated with miR-19b-3p and positively correlated with IGF-1. The predictive analysis identified binding sites for miR-19b-3p to IGF-1. The potential regulatory relationship between miR-19b-3p and IGF-1 was validated by in vitro and in vivo experiments. Moreover, the important role of miR-19b-3p in the regulation of OP was further demonstrated. It was concluded the inhibition of miR-19b-3p has a suppressive effect on the development of OP and the function of miR-19b-3p in OP is likely to be achieved by regulating the expression of the IGF-1 gene.

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“…Some studies have attempted to explain the pathogenesis of OP via different basic research methods and identify therapeutic targets by proteomic and metabonomic investigation of the pathogenic mechanism of OP. Previous studies [ 8 – 12 ] have shown that a variety of signaling pathways, such as the ER pathway, OPG/RANKL pathway, BMP-2/Smad pathway, classical Wnt/β-catenin signaling pathway, and PI3K/akt signaling pathway, can affect osteoblast proliferation and inhibit osteoclast differentiation.…”

Section: Introductionmentioning

confidence: 99%

Quantitative proteomics reveals the effect of Yigu decoction (YGD) on protein expression in bone tissue

Zhang

Yan

et al. 2021

Clin Proteom

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Background Osteoporosis (OP) is a systemic bone disease characterized by decreased bone mass, destruction of the bone tissue microstructure, increased bone brittleness and an increased risk of fracture. OP has a high incidence rate and long disease course and is associated with serious complications. Yigu decoction (YGD) is a compound prescription in traditional Chinese medicine that is used to treat OP. However, its mechanism in OP is not clear. This study used a tandem mass tag (TMT)quantitative proteomics method to explore the potential bone-protective mechanism of YGD in an osteoporotic rat model. Materials and methods A rat model of OP was established by ovariectomy. Eighteen 12-week-old specific-pathogen-free female Wistar rats weighing 220 ± 10 g were selected. The eighteen rats were randomly divided into 3 groups (n = 6 in each group): the normal, model and YGD groups. The right femurs from each group were subjected to quantitative biological analysis. TMT quantitative proteomics was used to analyze the proteins extracted from the bone tissue of rats in the model and YGD groups, and the differentially expressed proteins after intervention with YGD were identified as biologically relevant proteins of interest. Functional annotation correlation analysis was also performed to explore the biological function and mechanism of YGD. Result Compared with the model group, the YGD group showed significant upregulation of 26 proteins (FC > 1.2, P < 0.05) and significant downregulation of 39 proteins (FC < 0.833, P < 0.05). Four important targets involved in OP and 5 important signaling pathways involved in bone metabolism were identified. Conclusions YGD can significantly increase the bone mineral density (BMD) of osteoporotic rats and may play a therapeutic role by regulating target proteins involved in multiple signaling pathways. Therefore, these results improve the understanding of the OP mechanism and provide an experimental basis for the clinical application of YGD in OP treatment.

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miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways

Cited by 13 publications

References 28 publications

[Retracted] Exosome: Function and Application in Inflammatory Bone Diseases

[Retracted] Exosome: Function and Application in Inflammatory Bone Diseases

Down-regulation of miR-19b-3p enhances IGF-1 expression to induce osteoblast differentiation and improve osteoporosis

Quantitative proteomics reveals the effect of Yigu decoction (YGD) on protein expression in bone tissue

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