MiR-302c-3p suppresses invasion and proliferation of glioma cells via down-regulating metadherin (MTDH) expression

Abstract

Glioma is the most common malignant brain tumors with poor prognosis. The molecular events involved in the development and progression of glioma remain unclear. In this study, the expression levels of miR-302c-3p were examined in glioma tissues by qRT-PCR. The in vitro and in vivo functional effects of miR-302c-3p were examined further. Luciferase reporter assays were conducted to confirm the targeting associations. Results showed that the expression level of miR-302c-3p in glioma tissues was significantly lower than those in normal brain tissues (P < 0.001). The decreased expression of mi-302c-3p in glioma was positively associated with WHO grade (P < 0.001). Up-regulation of MTDH was also detected in glioma tumors compared with normal brain tissues (P = 0.0027) and is inversely correlated with miR-302c-3p expression (P = 0.003, R(2) = 0.4065). MTDH mRNA is a direct target of miR-302c-3p, whose ectopic expression decreases MTDH expression through binding to its 3'-untranslated region. Overexpression of miR-302c-3p results in a dramatic inhibition of glioma cells proliferation and invasion in vitro and in vivo. These data suggest that miR-302c-3p play a pivotal role in the progression of glioma by targeting MTDH and is a potential inhibitor in glioma treatment.

Long noncoding RNASNHG1promotes non‐small cell lung cancer progression by up‐regulatingMTDH viasponging miR‐145–5p

“…Other studies have demonstrated that miR-145-5p targeted the 39-UTR of MTDH and restored its expression (26,33,34). MTDH is a reported oncogene that is elevated in a variety of cancers and contributes to tumor growth and metastasis (35,36). In our present study, the expression patterns of MTDH mRNA and protein were in accordance with SNHG1 serving as a miR-145-5p reservoir.…”

Serum MicroRNA profile in patients with colon adenomas or cancer

“…Although not reported in colon cancer, some DEMs, such as miR-302c-3p [26], miR-487a [30] miR-638 [31], have been found involved in the survival, invasion or chemoresistance of several cancer cell lines. We also identified 26 novel significant DEMs in colon adenoma samples when compared with healthy subjects, some of which have previously reported as tumor-related miRNAs, such as miR-22 [37], miR-620 [38], miR-765 [39], and miR-1247 [40].…”

Association and in silico investigations of miR‐302c insertion/deletion variant as a novel biomarker with susceptibility to gastric cancer

“…Recently, a study reported that the expression of miR‐302c was downregulated among GC patients . Moreover, recent researches have suggested that miR‐302c is implicated in many cancer‐related biological mechanisms, including cell proliferation and invasion, by targeting several genes, such as CDK2, MTDH, AKT1, and IL8 . Altogether, the current study aimed to investigate the association of miR‐302c INDEL (rs199971565) with GC susceptibility as a putative prognostic biomarker.…”

“…It has been well evidenced that some miRNAs can function as oncogenes or tumor suppressors in various types of cancers by involving in several different biological processes including differentiation, cell proliferation, and apoptosis . As a member of the miR‐302 family, miR‐302c plays critical roles in the development of many cancers including GC, glioma, and hepatocellular carcinoma . Interestingly, a prognostic value in GC patients has been suggested for miR‐302c …”

“…10 As a member of the miR-302 family, miR-302c plays critical roles in the development of many cancers including GC, glioma, and hepatocellular carcinoma. [11][12][13] Interestingly, a prognostic value in GC patients has been suggested for miR-302c. 14 Various sequence variations located in mature and/or pre-miRNA sequences can influence miRNA biogenesis and function by changing the pre-miRNA maturation or miRNA-target interactions; this may alter numerous biological pathways and may be associated with several diseases, especially cancers.…”