miR‐30b‐5p up‐regulation related to the dismal prognosis for patients with renal cell cancer

Abstract

The diagnosis of renal cell carcinoma (RCC) is often made late since there is no early symptom, which thus results in dismal patient prognosis. As a result, new biomarkers are urgently needed and efforts should be made to identify their functions in predicting RCC prognosis. microRNAs (miRNAs) are a class of small noncoding RNAs that are about 20‐22 nucleotides in length, and they have been demonstrated to function as prognostic markers in numerous tumors. This study aimed to assess the role of miR‐30b‐5p in predicting the prognosis of RCC postoperatively. In this study, RNA was extracted from 284 formalin‐fixed and paraffin‐embedded kidney cancer tissue samples. After cDNA synthesis, real‐time quantitative PCR (RT‐qPCR) was adopted for detecting the relative miR‐30b‐5p level. Then, the Kaplan‐Meier method, Cox regression analysis, and the receiver operating characteristic curve analysis were applied in analyzing the miR‐30b‐5p effect on the prognosis for patients. Our findings indicated that, following adjustment for age, gender, tumor stage, and tumor size, patients with low miR‐30b‐5p expression had remarkably longer overall survival. Thus, the miR‐30b‐5p level might be related to RCC prognosis.

Hypoxic pancreatic cancer derived exosomal miR-30b-5p promotes tumor angiogenesis by inhibiting GJA1 expression

“…However, Wu et al 40 found that miR-30b-5p promoted the proliferation, migration and invasion of breast cancer cells. Moreover, the diagnostic and prognostic value of miR-30b-5p in peripheral blood and tumor tissues for renal cell carcinoma and breast cancer had been explored [41][42][43] . Our study demonstrated that hypoxic pancreatic cancer cells promoted angiogenesis through transferring exosomal miR-30b-5p to endothelial cells to regulate directly the expression of GJA1.…”