miR‐339‐5p downregulation contributes to Taxol resistance in small‐cell lung cancer by targeting α1,2‐fucosyltransferase 1

Gan, Chongzhi; Li, Gang; Luo, Qingsong; Li, Hongmin

doi:10.1002/iub.1679

Cited by 28 publications

(20 citation statements)

References 30 publications

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“… 16 MiR-339-5p could promote colony formation and attenuate apoptosis of lung carcinoma cell lines through targeting α1,2-fucosyltransferase 1 and regulation of the downstream protein Lewis y. 38 MiR-501-5p was upregulated in hepatocellular carcinoma specimens and cell lines. However, its potential role in lung cancer and other cancers was seldom investigated.…”

Section: Discussionmentioning

confidence: 99%

Application of serum microRNA-9-5p, 21-5p, and 223-3p combined with tumor markers in the diagnosis of non-small-cell lung cancer in Yunnan in southwestern China

Yang¹,

Chen²,

Zhou³

et al. 2018

OTT

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PurposeXuanwei City is located in late Permian coal-accumulating areas of the northeastern region of Yunnan Province. In China, morbidity and mortality from lung cancer are highest in Yunnan. Identifying useful circulating markers suitable for the diagnosis of lung cancer in this region is quite meaningful. In this study, we evaluated diagnostic roles of serum miR-9-5p, 21-5p, 223-3p, 135b-5p, 339-5p, and 501-5p in patients with non-small-cell lung cancer (NSCLC) in Yunnan. Moreover, we evaluated the diagnostic performance of several tumor markers, including carcinoembryonic antigen (CEA), cytokeratin 19 fragment 21-1 (CYFRA21-1), and squamous cell carcinoma-related antigen (SCC).MethodsQuantitative real-time polymerase chain reaction detected six miRNAs in the serum of 104 NSCLC patients and 50 cancer-free controls. Other markers, including CEA, CYFRA21-1, and SCC, in serum were also measured. The diagnostic ability of miRNAs and tumor markers was evaluated by receiver operating characteristic (ROC) curve analysis. The diagnostic performance of these serum markers was also evaluated in Xuanwei and non-Xuanwei subjects, because the etiological and the epidemiological characteristics of lung cancer in Xuanwei were quite different from those in other regions.ResultsSerum miR-9-5p, miR-21-5p, miR-223-3p, CEA, CYFRA21-1, and SCC were upregulated in NSCLC patients, compared with cancer-free controls. No significant difference was found in miR-135b-5p, miR-339-5p, and miR-501-5p expression. The area under ROC curves (AUCs) of miR-9-5p, miR-21-5p, miR-223-3p, CEA, CYFRA21-1, and SCC were 0.706, 0.765, 0.744, 0.749, 0.735, and 0.616, respectively. When combined, miRNAs and tumor markers yielded the highest diagnostic power, with AUC of 0.886, sensitivity of 82.69%, and specificity of 88.00%. In Xuanwei subjects, miR-223-3p and CEA may be suitable biomarkers to distinguish NSCLC from cancer-free states with AUCs of 0.752 and 0.791, respectively. The diagnostic power of the combination of miRNAs and tumor markers was still the highest in both subgroups (region: Xuanwei and non-Xuanwei; stages: I–II and III–IV).ConclusionSerum miR-9-5p, miR-21-5p, miR-223-3p, CEA, CYFRA21-1, and SCC could be potential diagnostic biomarkers for NSCLC patients in Yunnan. miRNAs and tumor markers should be combined to diagnose NSCLC, as it showed better ability for screening patients with NSCLC.

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Section: Discussionmentioning

confidence: 99%

Application of serum microRNA-9-5p, 21-5p, and 223-3p combined with tumor markers in the diagnosis of non-small-cell lung cancer in Yunnan in southwestern China

Yang¹,

Chen²,

Zhou³

et al. 2018

OTT

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“…Although miRNAs can serve either as oncogenes or tumor-suppressor genes, several studies have illustrated that miRNA expression is globally attenuated in tumor cells compared with normal tissue, implying that miRNA biogenesis might be suppressed during carcinogenesis. Recent studies have shown that miR-339-5p is downregulated and serves as a tumor suppressor gene in various cancers, including ovarian cancer 31 , colorectal cancer 32 , small-cell lung cancer 33 , non-small cell lung cancer 34 , breast cancer 35 , and hepatocellular carcinoma 36 . Indeed, we found that miR-339-5p was downregulated in OSCC clinical samples, which was significantly associated with increased TSPAN15 expression.…”

Section: Discussionmentioning

confidence: 99%

TSPAN15 interacts with BTRC to promote oesophageal squamous cell carcinoma metastasis via activating NF-κB signaling

Zhang

et al. 2018

Nat Commun

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Beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC) is crucial for the degradation of IκBα. Our previous transcriptome sequencing analysis revealed that tetraspanin 15 (TSPAN15) was significantly upregulated in clinical oesophageal squamous cell carcinoma (OSCC) tissues. Here, we show that high TSPAN15 expression in OSCC tissues is significantly associated with lymph node and distant metastasis, advanced clinical stage, and poor prognosis. Elevated TSPAN15 expression is, in part, caused by the reduction of miR-339-5p. Functional studies demonstrate that TSPAN15 promotes metastatic capabilities of OSCC cells. We further show that TSPAN15 specifically interacts with BTRC to promote the ubiquitination and proteasomal degradation of p-IκBα, and thereby triggers NF-κB nuclear translocation and subsequent activation of transcription of several metastasis-related genes, including ICAM1, VCAM1, uPA, MMP9, TNFα, and CCL2. Collectively, our findings indicate that TSPAN15 may serve as a new biomarker and/or provide a novel therapeutic target to OSCC patients.

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“…It was observed that miR-339-5p was significantly downregulated in Taxol-A549 cells compared with A549 cells, and this study suggested that miR-339-5p downregulation contributes to Taxol resistance in small-cell lung cancer. 26 In ovarian cancer, miR-339-5p is speculated to be a biomarker for inhibiting metastasis. 12 Zhou et al 14 demonstrated that the low-level expression of miR-339-3p was significantly associated with lymph node metastasis in patients with CRC.…”

Section: Discussionmentioning

confidence: 99%

<p>MicroRNA-339 inhibits human hepatocellular carcinoma proliferation and invasion via targeting ZNF689</p>

Zeng¹,

Zheng²,

Song³

et al. 2019

DDDT

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Background: Hepatocellular carcinoma (HCC) is the second leading cause of cancer mortality worldwide, however, the prognosis for HCC remains unsatisfactory. This study aimed to explore the role of miR-339-5p in HCC. Methods: We first used quantitative real-time PCR to examine the level of miR-339-5p in HCC tissues. Then we further adopted Western blotting assay, CCK8, cell invasion assays, apoptosis detection assay, and luciferase assay to analyze how it mediate the development of HCC. Results: We found that miR-339 is significantly decreased in primary HCC tissues. Overexpression of miR-339 in HCC cells remarkably suppressed proliferation and invasion and induced apoptosis. However, silencing miR-339 in HCC cells promoted proliferation and invasion, and reduced apoptosis. Moreover, we demonstrated that ZNF689 is a target of miR-339 and there is a negative correlation between miR-339 and ZNF689 expression in the HCC tissues. Overexpression of ZNF689 in miR-339-overexpressing HCC cells partially antagonized the inhibitory effects of miR-339. Conclusion: Our study revealed that miR-339 inhibits HCC growth through targeting oncoprotein ZNF689 and restoration of miR-339 might be feasible therapeutic strategy for HCC treatment.

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miR‐339‐5p downregulation contributes to Taxol resistance in small‐cell lung cancer by targeting α1,2‐fucosyltransferase 1

Cited by 28 publications

References 30 publications

Application of serum microRNA-9-5p, 21-5p, and 223-3p combined with tumor markers in the diagnosis of non-small-cell lung cancer in Yunnan in southwestern China

Application of serum microRNA-9-5p, 21-5p, and 223-3p combined with tumor markers in the diagnosis of non-small-cell lung cancer in Yunnan in southwestern China

TSPAN15 interacts with BTRC to promote oesophageal squamous cell carcinoma metastasis via activating NF-κB signaling

<p>MicroRNA-339 inhibits human hepatocellular carcinoma proliferation and invasion via targeting ZNF689</p>

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