miR-367 promotes tumor growth by inhibiting FBXW7 in NSCLC

Abstract

Abstract. miR-367 is one of the most abundant miRNAs in human embryonic stem cells (hESCs) and is mainly involved in maintaining the pluripotency of stem cells. However, its role in cancer development remains poorly understood. In the present study, we explored the function and mechanism of the endogenous miR-367 in non-small cell lung cancer (NSCLC). In the present study, we demonstrated that the level of miR-367 in NSCLC was significantly higher than that in adjacent normal tissues, and its upregulation was positively correlated with tumor size, tumor differentiation and tumor-node-metastasis (TNM) stage. miR-367 was an indicator of a poorer prognosis in NSCLC patients. Furthermore, overexpression of miR-367 significantly inhibited apoptosis and enhanced proliferation by promoting cell cycle transition from G1 to S phase. In contrast, knockdown of miR-367 markedly reversed the cellular events observed with miR-367 overexpression. Moreover, we identified that F-box and WD repeat domain-containing 7 (FBXW7) is a novel target of miR-367. It reverses the oncogenic effects of miR-367 by downregulating its substrates, c-Myc and c-Jun, in NSCLC cells. Finally, studies in vivo revealed that knockdown of miR-367 inhibited the growth of xenografts in the nude mice by increasing the expression of FBXW7. In summary, our findings indicate that miR-367 exerts tumorpromoting effect by negatively regulating FBXW7 in NSCLC, and it could become a potential therapeutic target for NSCLC intervention.

MiR-223 promotes oral squamous cell carcinoma proliferation and migration by regulating FBXW7

“…FBXW7 was proved to be a tumor suppressor in regulating tumors progression. A study showed that FBXW7 was identified as a potential target of miR-367 in regulating tumor growth of NSCLC [31]. Also, miR-92a mimic promoted the tumor growth of osteosarcoma via suppressing FBXW7 [32].…”

Research progress on the relationship between zinc deficiency, related microRNAs, and esophageal carcinoma

“…FBXW7 is a cell cycle protein that regulates the stability of several oncoproteins, including c-Myc, cyclin E, c-Jun, SREBP, Notch, and c-Myb, thereby acting as a vital tumor suppressor gene. 32 Multiple studies have reported that FBXW7 expression is regulated by several miRNAs, such as miR-223, miR-25, miR-182, and miR-367. 32,33 Kurashige et al examined miR-223 and FBXW7 expression levels in 109 pairs of primary EC tissue samples and corresponding normal esophageal epithelium samples obtained from patients who underwent esophageal resection without preoperative treatment.…”

“…32 Multiple studies have reported that FBXW7 expression is regulated by several miRNAs, such as miR-223, miR-25, miR-182, and miR-367. 32,33 Kurashige et al examined miR-223 and FBXW7 expression levels in 109 pairs of primary EC tissue samples and corresponding normal esophageal epithelium samples obtained from patients who underwent esophageal resection without preoperative treatment. The miR-223 expression level was significantly higher in primary cancer tissues than in corresponding normal tissues.…”

“…FBXW7 coordinates the ubiquitin‐dependent proteolysis of several critical cellular regulators, thereby controlling essential biological processes, including cell cycle, differentiation, and apoptosis. FBXW7 is a cell cycle protein that regulates the stability of several oncoproteins, including c‐Myc, cyclin E, c‐Jun, SREBP, Notch, and c‐Myb, thereby acting as a vital tumor suppressor gene . Multiple studies have reported that FBXW7 expression is regulated by several miRNAs, such as miR‐223, miR‐25, miR‐182, and miR‐367 …”

LSGSP: a novel miRNA–disease association prediction model using a Laplacian score of the graphs and space projection federated method

“…10 MiR-367 exerts a tumor-promoting effect through negatively regulating FBXW7 in non-small cell lung cancer (NSCLC), and it could be a potential therapeutic target for NSCLC intervention. 11 MiR-100 and miR-125b are associated with lymph node metastasis in early colorectal cancer, and may be novel biomarkers for the lymph node metastasis of early colorectal cancers with submucosal invasion. 12 Therefore, studying disease-related miRNAs is helpful for analyzing pathogenesis and exploring the rules related to diseases.…”