miR-450a-5p Eliminates MGO-Induced Insulin Resistance via Targeting CREB

Wei, Cuifeng; Li, Meng; Zhang, Yuting

doi:10.15283/ijsc19088

Cited by 7 publications

(6 citation statements)

References 31 publications

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“…Research has identified the potential function of miR-450a in human disease. Upregulated miR-450a eliminated methylglyoxal-induced insulin resistance via targeting cyclic AMP response element binding protein and may therefore be used as a potential target to improve insulin resistance and treat patients with diabetes-associated diseases (33). Furthermore, after glutamine discontinuation, miR-450a overexpression decreased the mitochondrial membrane potential but increased glucose uptake and cell viability, which are characteristic of less invasive cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Functional genetic variants in the 3'UTR of PTPRD associated with the risk of gestational diabetes mellitus

Huamin

et al. 2021

Exp Ther Med

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A previous study revealed that protein tyrosine phosphatase receptor type D (PTPRD) is highly associated with diabetes mellitus, particularly for type 2 diabetes, through a genome-wide association study. However, the influence of the human polymorphism in the 3'-untranslated region (3'-UTR) of PTPRD on gestational diabetes mellitus (GDM) has remained to be defined. The present study focused on the functional polymorphism located in the 3'-UTR of PTPRD and whether it is associated with the susceptibility to develop GDM. A total of 1,100 pregnant female patients aged between 28 and 36 years within gestational weeks 24-28 were recruited. The participants enrolled in the study comprised 500 cases of GDM and 600 normal controls. Based on the screening results, the single nucleotide polymorphism (SNP) rs56407701 exhibited the most significant difference and may increase the susceptibility to GDM. A prediction of target microRNAs (miRNAs/miRs) using the miRNA SNP database indicated that SNP rs56407701 may be bound by miR-450a, causing the suppression of PTPRD expression in subjects with the GC or CC genotype. In conclusion, The CC genotype of PTPRD rs56407701, which may be bound by miR-450a, may increase the susceptibility of Chinese Han females to GDM during pregnancy. The present study provided a theoretical basis for the SNP rs56407701 being a source of GDM susceptibility loci.

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Section: Discussionmentioning

confidence: 99%

Functional genetic variants in the 3'UTR of PTPRD associated with the risk of gestational diabetes mellitus

Huamin

et al. 2021

Exp Ther Med

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“…However, we found that fructose-fed rats for 4 weeks increase the levels of miR-450a-5p in total plasma ( Table S3 ). A recent study showed that the up-regulation of this miRNA improves insulin resistance in non-adipocyte cells ( Wei, Meng & Zhang, 2020 ). On the other hand, we showed that the abundance of miR-21-5p in EVs is high; however, we did not find changes in this miRNA in both cells and rats.…”

Section: Discussionmentioning

confidence: 99%

High fructose exposure modifies the amount of adipocyte-secreted microRNAs into extracellular vesicles in supernatants and plasma

Hernández-Díazcouder

González-Ramírez

Giacoman-Martínez

et al. 2021

PeerJ

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Background High fructose exposure induces metabolic and endocrine responses in adipose tissue. Recent evidence suggests that microRNAs in extracellular vesicles are endocrine signals secreted by adipocytes. Fructose exposure on the secretion of microRNA by tissues and cells is poorly studied. Thus, the aim of this study was to evaluate the effect of fructose exposure on the secretion of selected microRNAs in extracellular vesicles from 3T3-L1 cells and plasma from Wistar rats. Methods 3T3-L1 cells were exposed to 550 µM of fructose or standard media for four days, microRNAs levels were determined in extracellular vesicles of supernatants and cells by RT-qPCR. Wistar rats were exposed to either 20% fructose drink or tap water for eight weeks, microRNAs levels were determined in extracellular vesicles of plasma and adipose tissue by RT-qPCR. Results This study showed that fructose exposure increased the total number of extracellular vesicles released by 3T3-L1 cells (p = 0.0001). The levels of miR-143-5p were increased in extracellular vesicles of 3T3-L1 cells exposed to fructose (p = 0.0286), whereas miR-223-3p levels were reduced (p = 0.0286). Moreover, in plasma-derived extracellular vesicles, miR-143-5p was higher in fructose-fed rats (p = 0.001), whereas miR-223-3p (p = 0.022), miR-342-3p (p = 0.0011), miR-140-5p (p = 0.0129) and miR-146b-5p (p = 0.0245) were lower. Conclusion Fructose exposure modifies the levels of microRNAs in extracellular vesicles in vitro and in vivo. In particular, fructose exposure increases miR-143-5p, while decreases miR-223-3p and miR-342-3p.

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“…After screening all the miRNAs, we found that 9 downregulated miRNAs and 3 upregulated miRNAs were reported to have the ability to promote the migration of other cells (Fig. 5e) [20][21][22][23][24][25][26][27][28][29][30][31]. Further analysis revealed that 4 downregulated miRNAs were reported to regulate the NF-κB signaling pathway (Fig.…”

Section: Dp7-c and The Metabolite Pa Promoted DC Migration By Upregulating The Chemokine Receptor Cxcr2mentioning

confidence: 99%

Cholesterol modified DP7 and pantothenic acid induce dendritic cell homing to enhance the efficacy of dendritic cell vaccines

Zhang

Tang

et al. 2021

Mol Biomed

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Dendritic cell (DC)-based cancer vaccines have so far achieved good therapeutic effects in animal experiments and early clinical trials for certain malignant tumors. However, the overall objective response rate in clinical trials rarely exceeds 15%. The poor efficiency of DC migration to lymph nodes (LNs) (< 5%) is one of the main factors limiting the effectiveness of DC vaccines. Therefore, increasing the efficiency of DC migration is expected to further enhance the efficacy of DC vaccines. Here, we used DP7-C (cholesterol modified VQWRIRVAVIRK), which can promote DC migration, as a medium. Through multiomics sequencing and biological experiments, we found that it is the metabolite pantothenic acid (PA) that improves the migration and effectiveness of DC vaccines. We clarified that both DP7-C and PA regulate DC migration by regulating the chemokine receptor CXCR2 and inhibiting miR-142a-3p to affect the NF-κB signaling pathway. This study will lay the foundation for the subsequent use of DP7-C as a universal substance to promote DC migration, further enhance the antitumor effect of DC vaccines, and solve the bottleneck problem of the low migration efficiency and unsatisfactory clinical response rate of DC vaccines.

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miR-450a-5p Eliminates MGO-Induced Insulin Resistance via Targeting CREB

Cited by 7 publications

References 31 publications

Functional genetic variants in the 3'UTR of PTPRD associated with the risk of gestational diabetes mellitus

Functional genetic variants in the 3'UTR of PTPRD associated with the risk of gestational diabetes mellitus

High fructose exposure modifies the amount of adipocyte-secreted microRNAs into extracellular vesicles in supernatants and plasma

Cholesterol modified DP7 and pantothenic acid induce dendritic cell homing to enhance the efficacy of dendritic cell vaccines

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