miR-455-5p suppresses hepatocellular carcinoma cell growth and invasion via IGF-1R/AKT/GLUT1 pathway by targeting IGF-1R

Hu, Yiren; Yang, Zhangwei; Bao, Dandan; Ni, Jingwei; J, Lou

doi:10.1016/j.prp.2019.152674

Cited by 34 publications

(23 citation statements)

References 42 publications

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“…MirRNAs are RNA genes associated with miRNA class; the miRNAs are evolutionarily conserved small RNA molecules lacking coding potential, but play a role in post-transcriptional modification and in regulating cell differentiation, proliferation, and oxidative stress. 18 - 20 The top 5 MirRNA targets are displayed in Figure 4 .…”

Section: Resultsmentioning

confidence: 99%

“…In cell lines and tumor tissues of HCC patients, miR-455-5p an alias of Mir455 ( https://www.genecards.org/cgi-bin/carddisp.pl?gene=MIR455 ) was reported to be significantly suppressed, but when overexpressed in cell lines it was linked with decreased tumor functions and proliferation, and the suppression of insulin growth factor receptor (IGF-1R). 18 This led to suggestions by Hu et al 18 that mIR-455-5p is a potential target for therapeutic interventions in cancer because glycolysis is needed for tumor proliferation, and the suppression of IGF-1R disrupts AKT phosphorylation—a signaling pathway which promotes cell survival and growth, leading to glycolytic enzymes and glucose transporter (GLUT) downregulation. 18 mIR-455-5p decreased expression have also been linked with gastric cancer (GC) and colorectal cancer (CRC) and have been found to regulate RAB18 and RAF1 genes which are among its direct targets.…”

Section: Resultsmentioning

confidence: 99%

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Expression, Interaction, and Role of Pseudogene Adh6-ps1 in Cancer and other Disease Phenotypes

Nwadiugwu

2021

Bioinform Biol Insights

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Pseudogenes have been classified as functionless and their annotation is an ongoing problem. The Adh6-ps1—a mouse pseudogene belonging to the alcohol dehydrogenase gene complex (Adh) was analyzed to review the conservation, homology, expression, and interactions and identify any role it plays in disease phenotypes using bioinformatics databases. Results showed that Adh6-ps1 have 2 transcripts (processed and unprocessed) which may have emerged from a transposition and duplication event, respectively, and that induced inversions (Uox gene, In(3)11Rk) involving gene complexes associated with Adh6-ps1 have been implicated in a diverse range of diseases. Adh6-ps1 is highly conserved in vertebrates particularly rodents and expressed in the liver. The top 5 MirRNA targets were Mir455, Mir511, Mir1903, Mir361, and Mir669o markers. While much is unknown about Mir1903 and Mir669o, the silencing of Mir455 and Mir511 is linked with hepatocellular carcinoma (HCC), and Mir361 is implicated in endometrial cancers. Given the identified MirRNA interactions with Adh6-ps1 and its expression in HCC and reproductive systems, it may well have a role in tumorigenesis and disease phenotypes. Nonetheless, further studies are required to establish these facts to add to the growing efforts to understand pseudogenes and their potential involvement in disease conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Expression, Interaction, and Role of Pseudogene Adh6-ps1 in Cancer and other Disease Phenotypes

Nwadiugwu

2021

Bioinform Biol Insights

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“…This miRNA is able to impair HCC cell malignancy via suppression of insulin growth factor receptor expression, thereby disrupting glycolysis. 26 However, in glioma patients, the miRNA was noted to be significantly upregulated, and an increasing expression of this miRNA was associated with decreasing five-year overall survival rates. 27 The specific mechanism of miR-455-3p, with respect to PAAD, is still unclear.…”

Section: Discussionmentioning

confidence: 99%

A Novel miRNA–mRNA Axis Involves in Regulating Transcriptional Disorders in Pancreatic Adenocarcinoma

Shang¹,

Shi²,

Taule³

et al. 2021

CMAR

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Background Currently, there is still a lack of understanding about the mechanism and therapeutic targets of pancreatic adenocarcinoma (PAAD). The potential of miRNA–mRNA networks for the identification of regulatory mechanisms involved in PAAD development remains unexplored. Methods We compared differentially expressed miRNAs (DEMIs) and differentially expressed genes (DEGs) in PAAD and normal tissues from the Gene Expression Omnibus (GEO) database. Transcription factors (TFs) were obtained from FunRich. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DEGs and DEMIs were implemented using Database for Annotation, Visualization and Integrated Discovery (DAVID). Then, key miRNAs and targeted mRNAs were identified by assessment of their expression and prognosis in UALCAN and Kaplan–Meier plotters. In the last step, the candidate miRNA–mRNA selected was confirmed by real-time quantitative polymerase chain reaction (qRT-PCR). Results We distinguished 62 significant DEMIs, 1314 upregulated DEGs, and 1110 downregulated DEGs. The top 10 TFs were identified. In total, there were 160 hub genes obtained by intersecting the set of 2224 predicted targets with the set of significant DEGs. And we selected 8 key miRNAs. Furthermore, low expression of miR-455-3p in PAAD tissue was closely connected with poor prognosis, and only 5 target mRNAs were predicted to be increased in PAAD tissue with poor prognosis. Therefore, a novel miRNA–hub gene regulatory network in PAAD was constructed. Finally, in vitro experiments indicated that miR-455-3p expression was decreased in PAAD sample. HOXC4, DLG4, DYNLL1 and FBXO45 were validated by qRT-PCR as highly probable targets of miR-455-3p. Conclusion A novel miRNA–mRNA axis has been discovered that may be involved in the regulation of transcriptional disorders and affected the survival of PAAD patients, which would provide a novel strategy for the treatment of PAAD.

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“…(1) Noncoding RNA Regulates GLUT1 to Participate in Glucose Uptake of Hepatoma Cells. LINC01638 lncRNA [35], lncRNA HOTAIR [36], miR-181a-5p [37], and MiR-505 [38] increase the uptake of glucose by cancer cells by upregulating the expression of GLUT1 on the surface of HCC cells and promote the proliferation of hepatocellular carcinoma cells; lncRNA SLC2A1-AS1 [39], miR-342-3p [40], miR-455-5p [41], and miR-328-3p [42] can reduce the expression of GLUT1 on the surface of HCC cells. Inhibiting the uptake of glucose by cancer cells inhibits the proliferation of hepatocellular carcinoma cells.…”

Section: Noncoding Rna Is Involved In Regulationmentioning

confidence: 99%

Mechanism, Clinical Significance, and Treatment Strategy of Warburg Effect in Hepatocellular Carcinoma

Chen

Liu

et al. 2021

Journal of Nanomaterials

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Hepatocellular carcinoma (HCC) is a primary malignancy of the liver and the third leading cause of cancer death worldwide. The incidence of HCC accounts for more than 90% of primary HCC. Like most solid malignancies, the occurrence and development of HCC are closely related to the Warburg effect. The Warburg effect of HCC is mainly manifested as increased glucose uptake by HCC cells, increased glycolysis, restricted mitochondrial oxidative phosphorylation, increased pentose phosphate pathway in HCC cells, and increased glutamine decomposition. As the contribution of glycolysis to the total ATP of tumor cells generally does not exceed 50% to 60%, oxidative phosphorylation (OXPHOS) still makes a considerable contribution to the ATP of tumor cells. In some cases, there will be an anti-Warburg effect. HCC Warburg effect is closely related to HCC cell proliferation, apoptosis, immune escape, migration and invasion, chemotherapy resistance, and treatment failure. The mechanism of the Warburg effect in HCC is complex, involving the expression of stimulating the key glycolysis enzymes by hypoxia-inducible factor-1(HIF-1), the activation of oncogenes and the inactivation of tumor suppressor genes, the continuous activation of related signaling pathways, the participation of noncoding RNA, and the rate of metabolism gene mutation of enzyme. This article synthetically discusses the characteristics of glucose metabolism in HCC cells, the mechanism of Warburg effect, clinical significance, and corresponding treatment strategies and provides new perspectives for the prevention and treatment of HCC.

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miR-455-5p suppresses hepatocellular carcinoma cell growth and invasion via IGF-1R/AKT/GLUT1 pathway by targeting IGF-1R

Cited by 34 publications

References 42 publications

Expression, Interaction, and Role of Pseudogene Adh6-ps1 in Cancer and other Disease Phenotypes

Expression, Interaction, and Role of Pseudogene Adh6-ps1 in Cancer and other Disease Phenotypes

A Novel miRNA–mRNA Axis Involves in Regulating Transcriptional Disorders in Pancreatic Adenocarcinoma

Mechanism, Clinical Significance, and Treatment Strategy of Warburg Effect in Hepatocellular Carcinoma

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