miR-458b-5p regulates ovarian granulosa cells proliferation through Wnt/β‐catenin signaling pathway by targeting catenin beta-1

Abstract

Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA play an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear. Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-Catenin pathway. The Cell Counting Kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and Annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect the mRNA and protein expression levels. Results: We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells (TCs) of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and have no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule (CD44) and matrix metallopeptidase 7 (MMP7), which are the downstream effector of A c c e p t e d A r t i c l e CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation. Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development.

β-Catenin regulates ovarian granulosa cell cycle and proliferation in laying hens by interacting with TCF4

“…β-Catenin, encoded by CTNNB1 , is a crucial regulator in the Wnt/β-catenin signaling pathway. Numerous studies have demonstrated that the Wnt/β-catenin signaling pathway plays an important role in the regulation of myriad cell functions including proliferation, invasion, metastasis, apoptosis, differentiation, steroid synthesis, as well as others ( Wang et al, 2021 ). Guo et al reported that by upregulating the Wnt/β-catenin pathway, they were able to promote the proliferation of murine granulosa cells ( Guo et al, 2018 ).…”