MiR-485 targets the DTX4 gene to regulate milk fat synthesis in bovine mammary epithelial cells

Liu, Juan; Ping, Jiang; Iqbal, Ambreen; Ali, Shaokat; Zhen, Gao; Pan, Zhifang; Xia, Lixin; Yin, Fuquan; Zhao, Zhihui

doi:10.1038/s41598-021-87139-5

Cited by 7 publications

(4 citation statements)

References 25 publications

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“…Most of the research on miR-485 is related to human cancer. A recent report discovered that miR-485 affected the content of triglycerides (TGs) and cholesterol (CHOL) in milk fat by targeting the DTX4 gene ( Liu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of microRNAs related to unsaturated fatty acid synthesis by interfering bovine adipocyte ACSL1 gene

Bai²,

Li³

et al. 2022

Front. Genet.

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Long-chain fatty acyl-CoA synthase 1 (ACSL1) plays a vital role in the synthesis and metabolism of fatty acids. The proportion of highly unsaturated fatty acids in beef not only affects the flavor and improves the meat’s nutritional value. In this study, si-ACSL1 and NC-ACSL1 were transfected in bovine preadipocytes, respectively, collected cells were isolated on the fourth day of induction, and then RNA-Seq technology was used to screen miRNAs related to unsaturated fatty acid synthesis. A total of 1,075 miRNAs were characterized as differentially expressed miRNAs (DE-miRNAs), of which the expressions of 16 miRNAs were upregulated, and that of 12 were downregulated. Gene ontology analysis indicated that the target genes of DE-miRNAs were mainly involved in biological regulation and metabolic processes. Additionally, KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis identified that the target genes of DE-miRNAs were mainly enriched in metabolic pathways, fatty acid metabolism, PI3K-Akt signaling pathway, glycerophospholipid metabolism, fatty acid elongation, and glucagon signaling pathway. Combined with the previous mRNA sequencing results, several key miRNA-mRNA targeting relationship pairs, i.e., novel-m0035-5p—ACSL1, novel-m0035-5p—ELOVL4, miR-9-X—ACSL1, bta-miR-677—ACSL1, miR-129-X—ELOVL4, and bta-miR-485—FADS2 were screened via the miRNA-mRNA interaction network. Thus, the results of this study provide a theoretical basis for further research on miRNA regulation of unsaturated fatty acid synthesis in bovine adipocytes.

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Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of microRNAs related to unsaturated fatty acid synthesis by interfering bovine adipocyte ACSL1 gene

Bai²,

Li³

et al. 2022

Front. Genet.

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“…In addition, Shen et al ( 2019) noted an increased triglyceride content when miR-124a was overexpressed in mammary epithelial cells, as well as an increase in free fatty acids [55]. Recently, the ability of miR-485 to regulate the synthesis of triglycerides, non-esterified fatty acids, and cholesterol in the mammary epithelial cells of cows, by inhibiting the expression of the DTX4 gene, which is a PPAR-γ transcription factor, was observed [141]. Additionally, recently demonstrated the regulation of the synthesis of polyunsaturated fatty acids by miR-193a-5p through the modulation of fatty acid desaturase 1 (FADS1), leading the authors to conclude a role for this miRNA in regulating the amount of milk fatty acids in the epithelial cells of the mammary glands of cattle [142].…”

Section: Lipid Metabolismmentioning

confidence: 99%

The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep

Dysin

Barkova

Pozovnikova

2021

ncRNA

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Milk is an integral and therefore complex structural element of mammalian nutrition. Therefore, it is simple to conclude that lactation, the process of producing milk, is as complex as the mammary gland, the organ responsible for this biochemical activity. Nutrition, genetics, epigenetics, disease pathogens, climatic conditions, and other environmental variables all impact breast productivity. In the last decade, the number of studies devoted to epigenetics has increased dramatically. Reports are increasingly describing the direct participation of microRNAs (miRNAs), small noncoding RNAs that regulate gene expression post-transcriptionally, in the regulation of mammary gland development and function. This paper presents a summary of the current state of knowledge about the roles of miRNAs in mammary gland development, health, and functions, particularly during lactation. The significance of miRNAs in signaling pathways, cellular proliferation, and the lipid metabolism in agricultural ruminants, which are crucial in light of their role in the nutrition of humans as consumers of dairy products, is discussed.

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“…It mainly acts by base-pairing with the complementary sequence of the 3'UTR region of the target gene, thereby promoting the degradation of mRNA and/or inhibiting its translation [13]. miRNAs are mRNA-inhibitory factors that regulate various physiological and cellular processes, including fat formation, cell proliferation, apoptosis, triglyceride synthesis, and lipolysis, via post-transcriptional processing [14]. In recent years, there has been increasing evidence that lncRNAs can regulate genes by directly binding the mRNA competing with miRNAs, thus regulating physiological processes, similar to endogenous RNA (ceRNAs) [15].…”

Section: Introductionmentioning

confidence: 99%

lncRNA MSTRG4710 Promotes the Proliferation and Differentiation of Preadipocytes through miR-29b-3p/IGF1 Axis

Tang,

Jiang,

Shao

et al. 2023

IJMS

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Obesity, a major global health issue, is increasingly associated with the integral role of long non-coding RNA (lncRNA) in adipogenesis. Recently, we found that lncRNA-MSTRG4710 was highly expressed in the liver of rabbits fed a high-fat diet, but whether it is involved in lipid metabolism remains unclear. A series of experiments involving CCK-8, EDU, qPCR, and Oil Red O staining demonstrated that the overexpression of MSTRG4710 stimulated the proliferation and differentiation of preadipocytes while its knockdown inhibited these processes. Bioinformatics analysis showed that miR-29b-3p was a potential target gene of MSTRG4710, and IGF1 was a downstream target gene of miR-29b-3p. Luciferase reporter gene analysis and qPCR analysis confirmed that miR-29b-3p was a potential target gene of MSTRG4710, and miR-29b-3p directly targeted the 3′UTR of IGF1. The overexpression of miR-29b-3p was observed to regulate IGF1 protein and mRNA levels negatively. Additionally, a total of 414 known differentially expressed genes between the miR-29b-3p mimic, miR-29b-3p negative control (NC), siMSTRG4710, and siMSTRG4710-NC group were screened via transcriptome sequencing technology. The GO- and KEGG-enriched pathways were found to be related to lipid metabolism. The study also established that miR-29b-3p targets IGF1 to inhibit preadipocyte proliferation and differentiation. Notably, IGF1 knockdown significantly reduced preadipocyte proliferation and differentiation. Furthermore, co-transfection of pcDNA3.1(+)-MSTRG4710 and mimics into rabbit preadipocytes revealed that the mimics reversed the promotional effect of pcDNA3.1(+)-MSTRG4710. In conclusion, these results uncover that MSTRG4710 positively regulated cell proliferation and adipogenesis by the miR-29b-3p/IGF1 axis. Our findings might provide a new target for studying adipogenesis in rabbit preadipocytes and obesity.

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MiR-485 targets the DTX4 gene to regulate milk fat synthesis in bovine mammary epithelial cells

Cited by 7 publications

References 25 publications

Transcriptional analysis of microRNAs related to unsaturated fatty acid synthesis by interfering bovine adipocyte ACSL1 gene

Transcriptional analysis of microRNAs related to unsaturated fatty acid synthesis by interfering bovine adipocyte ACSL1 gene

The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep

lncRNA MSTRG4710 Promotes the Proliferation and Differentiation of Preadipocytes through miR-29b-3p/IGF1 Axis

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