MiR-503 inhibited cell proliferation of human breast cancer cells by suppressing CCND1 expression

Long, Jianting; Ou, Caiwen; Xia, Haoming; Zhu, Yifan; Liu, Dayue

doi:10.1007/s13277-015-3623-8

Cited by 62 publications

(53 citation statements)

References 31 publications

(32 reference statements)

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“…One previous study in MCF-7 cells demonstrated that overexpression of miR-503 was able to inhibit cell cycle progression through repression of CCND1 (Long et al 2015). Based on our findings that miR-503 targets ZNF217, which promotes cell cycle progression , we hypothesized that the anti-proliferative effect of miR-503 resulted from a G1 arrest.…”

Section: The Role Of Mir-503 and Znf217 In Cellular Proliferation Andmentioning

confidence: 62%

“…One of the predicted targets, CCND1 (Fig. 5B), has already been validated as a target of miR-503 and the repression of CCND1 by miR-503 has been reported to inhibit proliferation in breast cancer cell lines (Long et al 2015). Another predicted target, ZNF217 (Fig.…”

Section: Computational Prediction Of Mirna-mrna Regulatory Interactionsmentioning

confidence: 89%

“…Several studies have recently shown that miR-503 may be a potent tumor suppressive miRNA. One such study showed that miR-503 targets CCND1 and reduces proliferation in both MCF-7 and MDA-MB-231 cells (an ER [−] Claudin-low model) (Long et al 2015). Others have shown that miR-503 reduces proliferation and metastasis in prostate cancer , in osteosarcoma (Chong et al 2014), and in hepatocellular carcinoma .…”

Section: Discussionmentioning

confidence: 99%

“…The role of miR-503 in opposing proliferation has been very recently investigated in breast cancer (Long et al 2015;Polioudakis et al 2015), prostate cancer , and osteosarcoma (Chong et al 2014). One previous study in MCF-7 cells demonstrated that overexpression of miR-503 was able to inhibit cell cycle progression through repression of CCND1 (Long et al 2015).…”

Section: The Role Of Mir-503 and Znf217 In Cellular Proliferation Andmentioning

confidence: 99%

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An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells

Baran-Gale

Purvis

Sethupathy

2016

RNA

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Estrogen receptor α (ERα) is an important biomarker of breast cancer severity and a common therapeutic target. In response to estrogen, ERα stimulates a dynamic transcriptional program including both coding and noncoding RNAs. We generate a fine-scale map of expression dynamics by performing a temporal profiling of both messenger RNAs (mRNAs) and microRNAs (miRNAs) in MCF-7 cells (an ER+ model cell line for breast cancer) in response to estrogen stimulation. We identified three primary expression trends-transient, induced, and repressed-that were each enriched for genes with distinct cellular functions. Integrative analysis of mRNA and miRNA temporal expression profiles identified miR-503 as the strongest candidate master regulator of the estrogen response, in part through suppression of ZNF217-an oncogene that is frequently amplified in cancer. We confirmed experimentally that miR-503 directly targets ZNF217 and that overexpression of miR-503 suppresses MCF-7 cell proliferation. Moreover, the levels of ZNF217 and miR-503 are associated with opposite outcomes in breast cancer patient cohorts, with high expression of ZNF217 associated with poor survival and high expression of miR-503 associated with improved survival. Overall, these data indicate that miR-503 acts as a potent estrogen-induced candidate tumor suppressor miRNA that opposes cellular proliferation and has promise as a novel therapeutic for breast cancer. More generally, our work provides a systemslevel framework for identifying functional interactions that shape the temporal dynamics of gene expression.

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Section: The Role Of Mir-503 and Znf217 In Cellular Proliferation Andmentioning

confidence: 62%

Section: Computational Prediction Of Mirna-mrna Regulatory Interactionsmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: The Role Of Mir-503 and Znf217 In Cellular Proliferation Andmentioning

confidence: 99%

See 2 more Smart Citations

An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells

Baran-Gale

Purvis

Sethupathy

2016

RNA

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“…Több daganatban tumorszuppresszori szerepét feltételezik, pl. hepatocellularis és emlőrák, glioblastoma multiforme esetén az IGF1 receptorának, valamint a ciklin-D1-és -D3-nak a gátlása révén csökkentette a sejtek proliferáci-óját, migrációját és az angiogenezist [25][26][27].…”

Section: A Mir-503unclassified

A mikro-RNS-ek patogenetikai és diagnosztikai szerepe mellékvesekéreg-carcinomában

et al. 2018

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A mellékvesekéreg daganatos elváltozásai a népesség jelentős részét érintik. E daganatok többsége jóindulatú, hormonálisan inaktív mellékvesekéreg-adenoma. A ritka hormontermelő mellékvesekéreg-adenomák súlyos klinikai kö-vetkezményekkel járó betegségeket okoznak, a kifejezetten ritka mellékvesekéreg-carcinoma prognózisa ugyanakkor előrehaladott stádiumaiban nagyon rossz. E daganatok patogenezise a mai napig nem teljesen tisztázott. A mikro-RNS-ek kisméretű nem kódoló RNS-molekulák, amelyek a génexpresszió poszttranszkripciós szabályozása révén jelentős szerepet töltenek be számos alapvető sejtbiológiai folyamatban. Megváltozott kifejeződésüket számos daganatban leírták. Több szöveti mikro-RNS, így a miR-483-5p, a miR-503, a miR-210, a miR-335 és a miR-195 megváltozott kifejeződését találták jó-és rosszindulatú mellékvese-daganatok között, és ezeknek patogenetikai jelentősé-gük is lehet. A mikro-RNS-ek szövetspecifikus és stabil kifejeződésüknek köszönhetően a diagnosztikában is felhasználhatók lehetnek. Tekintettel arra, hogy a mellékvesekéreg-daganatok szövettani diagnózisa nehéz, a szöveti mikro-RNS-ek segíthetnek a malignitás megállapításában is. Új eredmények igazolták, hogy a mikro-RNS-ek külön-böző testnedvekbe is kiválasztódnak, ami a folyadékbiopszia módszere révén biomarkerként történő felhasználható-ságukat vetíti előre. Összefoglaló cikkünkben a mikro-RNS-ek mellékvesekéreg-daganatokban betöltött patogenetikai szerepét és diagnosztikai alkalmazási lehetőségeit tekintjük át. Orv Hetil. 2018; 159(7): 245-251. Kulcsszavak: mellékvesekéreg-rák, mikro-RNS, szöveti, keringő, extracellularis vesicula Pathogenic and diagnostic roles of microRNAs in adrenocortical tumoursAdrenocortical tumours are quite prevalent. Most of these tumours are benign, hormonally inactive adrenocortical adenomas. Rare hormone-secreting adrenocortical adenomas are associated with severe clinical consequences, whereas the prognosis of the rare adrenocortical cancer is rather poor in its advanced stages. The pathogenesis of these tumours is only partly elucidated. MicroRNAs are small, non-coding RNA molecules that are pivotal in the regulation of several basic cell biological processes via the posttranscriptional regulation of gene expression. Their altered expression has been described in many tumours. Several tissue microRNAs, such as miR- 483-5p, miR-503, miR-210, miR-335 and miR-195 were found to be differentially expressed among benign and malignant adrenocortical tumours, and these could also have pathogenic relevance. Due to their tissue specific and stable expression, microRNAs can be exploited in diagnostics as well. As the histological diagnosis of adrenocortical malignancy is difficult, microRNAs might be of help in the establishment of malignancy. Novel data show that microRNAs are secreted in various body fluids, projecting their applicability as biomarkers as part of liquid biopsy. In this review, we attempt to present a synopsis on the pathogenic relevance of microRNAs in adrenocortical tumours and their potential di...

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MicroRNA‐503‐5p inhibits stretch‐induced osteogenic differentiation and bone formation

Liu

et al. 2016

Cell Biology International

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Cyclical stretch-induced bone formation during orthodontic treatment is a complex biological process modulated by various factors including miRNAs and their targeted-gene network. However, the miRNA expression profile and their roles in osteogenic differentiation of bone mesenchymal stem cells (BMSCs) exposed to mechanical stretch remains unclear. Here, we use the miRNA microarray assay to screen for mechano-sensitive miRNAs during stretch-induced osteogenic differentiation of BMSCs and identified that nine miRNAs were differentially expressed between stretched and control BMSCs. Furthermore, miR-503-5p, which was markedly downregulated in both microarray assay and qRT-PCR assay were selected for further functional verification. We found that overexpression of miR-503-5p in BMSCs attenuated stretch-induced osteogenic differentiation while the effect was reversed by miR-503-5p inhibition treatment. In vivo studies, overexpression of miR-503-5p with specific agomir decreased Runx2, ALP mRNA, and protein expression, decreased osteoblast numbers and osteoblastic bone formation in the OTM tension sides. In conclusion, our study revealed that miR-503-5p functions as the mechano-sensitive miRNA and inhibits BMSCs osteogenic differentiation subjected to mechanical stretch and bone formation in OTM tension sides.

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MiR-503 inhibited cell proliferation of human breast cancer cells by suppressing CCND1 expression

Cited by 62 publications

References 31 publications

An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells

An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells

A mikro-RNS-ek patogenetikai és diagnosztikai szerepe mellékvesekéreg-carcinomában

MicroRNA‐503‐5p inhibits stretch‐induced osteogenic differentiation and bone formation

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