MiR-519a functions as a tumor suppressor and is negatively associated with poor prognosis of non-small cell lung cancer

Han

Technol Cancer Res Treat

et al. 2020

Objective: MicroRNAs (miRNAs) have been found to play important roles in the development of non-small cell lung carcinoma (NSCLC). The aim of this study was to analyze the expression and clinical value of serum miR-185 in NSCLC. Methods: Serum miR-185 levels were detected in 146 NSCLC patients, 50 patients with carcinoma in situ, 25 patients with non-malignant lung diseases (NMLD), and 80 healthy controls using quantitative reverse transcription PCR. The correlation between serum miR-185 level and clinical status of NSCLC was explored. Results: The results revealed that serum miR-185 expression was progressively decreased in healthy controls, patients with NMLD, patients with carcinoma in situ and NSCLC patients. In addition, compared to carcinoembryonic antigen (CEA), serum miR-185 demonstrated better diagnostic accuracy for discriminating patients with carcinoma from healthy controls, NSCLC patients from healthy controls and NSCLC patients from patients with carcinoma in situ. In addition, serum miR-185 levels were significantly elevated in post-treated samples compared to the pre-treated samples. Moreover, reduced serum miR-185 was closely associated with unfavorable clinicopathological parameters and worse survival. Univariate and multivariate cox regression analysis confirmed that serum miR-185 was an independent prognostic indicator for NSCLC. Conclusions: Collectively, our findings have demonstrated that serum miR-185 might serve as a promising and robust biomarker for the early detection and prognosis prediction of NSCLC.

Section: Introductionmentioning

confidence: 99%

Serum miR-185 Is a Diagnostic and Prognostic Biomarker for Non-Small Cell Lung Cancer

Han

Technol Cancer Res Treat

et al. 2020

“…However, in breast cancer, miR‐519a‐3p was downregulated and inhibited MCF‐7 cell proliferation via targeting HUR 27 . MiR‐519a‐3p was also downregulated in nonsmall cell lung cancer and inhibited the proliferation, migration, and invasion of nonsmall cell lung cancer cells by directly targeting STAT3 28 . The above are the reports on miR‐519a‐3p, but there are few studies on miR‐519a‐5p.…”

Section: Discussionmentioning

confidence: 96%

“…27 MiR-519a-3p was also downregulated in nonsmall cell lung cancer and inhibited the proliferation, migration, and invasion of nonsmall cell lung cancer cells by directly targeting STAT3. 28 The above are the reports on miR-519a-3p, but there are few studies on miR-519a-5p. One paper reported that miR-519a-5p has an anti-Warburg effect on ovarian cancer by targeting HIF-1α.…”

Section: Arhgap29 Was the Downstream Target Of Mir-519a-5pmentioning

confidence: 99%

circTMEM181 upregulates ARHGAP29 to inhibit hepatocellular carcinoma migration and invasion by sponging miR‐519a‐5p

Chen

et al. 2022

Hepatology Research

Aim Circular RNAs (circRNAs) are a novel class of noncoding RNAs and are conserved in various species. Although numerous circRNAs have been identified, their role in cancer remains unclear. Methods The expression of circTMEM181 in 90 paired human hepatocellular carcinoma (HCC) and adjacent nontumor tissues were detected using quantitative reverse transcription–polymerase chain reaction. Transwell assay was performed for functional analysis of HCC cell migration and invasion. Luciferase reporter assay was used to verify the combination of circTMEM181 and miR‐519a‐5p. Results In this study, we identified a novel circRNA, named circTMEM181, was downregulated in HCC tissues. Decreased expression of circTMEM181 was associated with shorter overall survival of patients with HCC. CircTMEM181 overexpression reduced HCC cell migration and invasion abilities, while circTMEM181 knockdown increased cell motility. Mechanically, circTMEM181 could directly bind to miR‐519a‐5p and subsequently upregulate ARHGAP29 protein expression. Conclusion These data provide the first evidence of clinical significance and function of circTMEM181, and suggest the circTMEM181/miR‐519a‐5p/ARHGAP29 axis in HCC development.

“…miR-1181 was considered to be able to predict the prognosis of NSCLC, as its upregulation was associated with a poorer survival rate of patients with NSCLC. Accumulating evidence has demonstrated the prognostic value of miRNAs in several cancer types (26)(27)(28), and a variety of miRNAs, such as miR-519a, miR-378 and miR-1246, have been considered as prognostic biomarkers for NSCLC (29)(30)(31). In the present study, the results of the Cox regression analysis indicated that miR-1181 and the TNM stage were independent prognostic indicators for NSCLC.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of miR‑1181 in non‑small cell lung cancer and its regulatory effect on tumor progression

Wang

et al. 2021

Exp Ther Med

Non-small cell lung cancer (NSCLC) is one of the most important causes of cancer-related death. miR-1181 has been reported to have roles in various cancer types and its function in the progression of NSCLC was investigated in the present study. A total of 118 patients with NSCLC were recruited and their tumor tissues were collected. The expression of miR-1181 in NSCLC tissues and cells was detected by reverse transcription-quantitative PCR. The prognostic value of miR-1181 was evaluated by Kaplan-Meier and Cox regression analysis and the roles of miR-1181 in cell proliferation, migration and invasion of NSCLC were analyzed by Cell Counting Kit-8 and Transwell assays. miR-1181 was indicated to be upregulated in NSCLC tissues and cell lines, and to be associated with lymph node metastasis and the TNM stage of patients. Patients with high miR-1181 expression had a poorer prognosis than those with low miR-1181 expression. miR-1181 levels and TNM stage were determined to be two independent prognostic factors for NSCLC. In addition, overexpression of miR-1181 exerted enhancing effects on cell proliferation, migration and invasion of NSCLC, while its knockdown inhibited these cellular processes. In conclusion, upregulation of miR-1181 in NSCLC was associated with lymph node metastasis and TNM stage of patients and was indicative of poor prognosis. miR-1181 was indicated to exert promoting effects on cell proliferation, migration and invasion of NSCLC cells and to be involved in tumor progression, providing novel insight for the development of biomarkers and therapies for NSCLC.