MiR-539-5p negatively regulates migration of rMSCs induced by Bushen Huoxue decoction through targeting Wnt5a

Hu, Liuchao; Wang, Bin; Wu, Zhifang; Chen, Yingxiong; Yu, Lijuan; Zhu, J.-Y.; Shen, Wei; Chen, Chen; Chen, Dongfeng; Li, Gang; Xu, Liangliang

doi:10.7150/ijms.33437

Cited by 6 publications

(3 citation statements)

References 33 publications

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“…For example, oily extract from Catharmus tinctorius (1-50 µg/ml) promotes rat BMSC migration through Rho-associated protein kinase 2 (ROCK2) signaling in vitro . In addition, Bushen Huoxue decoction (100 µg/ml), a mixture comprised of eleven Chinese herbs, increase rat BMSC migration in vitro by regulating MiR-539-5p miRNA (Hu et al, 2019) and activating Wnt5a-related cell motility (Shen et al, 2018). Despite these observations, none of the individual compounds responsible have been identified from these extracts.…”

Section: Guanxin Danshen Formulationmentioning

confidence: 99%

Recruitment of Mesenchymal Stem Cells to Damaged Sites by Plant-Derived Components

Maeda

2020

Front. Cell Dev. Biol.

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Mesenchymal stem cells (MSCs) are capable of differentiating into a limited number of diverse cells and secrete regenerative factors that contribute to the repair of damaged tissue. In response to signals emitted by tissue damage, MSCs migrate from the bone marrow and area surrounding blood vessels within tissues into the circulating blood, and accumulate at the site of damage. Hence, MSC transplantation therapy is beginning to be applied to the treatment of various intractable human diseases. Recent medicinal plants studies have shown that plant-derived components can activate cell functions. For example, several plant-derived components activate cell signaling pathways, such as phosphatidylinositol 3-kinase and mitogen-activated protein kinase (MAPK), enhance expression of the CXCL12/CXCR4 axis, stimulate extracellular matrix remodeling, and consequently, promote cell migration of MSCs. Moreover, plant-derived components have been shown to promote recruitment of MSCs to damaged tissues and enhance healing in disease models, potentially advancing their therapeutic use. This article provides a comprehensive review of several plant-derived components that activate MSC migration and homing to damaged sites to promote tissue repair.

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Section: Guanxin Danshen Formulationmentioning

confidence: 99%

Recruitment of Mesenchymal Stem Cells to Damaged Sites by Plant-Derived Components

Maeda

2020

Front. Cell Dev. Biol.

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“…MiR-27a mediated the protective effect of paclitaxel in the damaged liver from septic mice by suppressing NF-kB/TAB3 signaling pathway [13]. MiR-539-5p has been shown as a potent regulator in choroidal neovascularization and the migration of mesenchymal stem cells in fracture healing [14,15]. However, whether it may exert beneficial effect in ALI remains unclear.…”

Section: Introductionmentioning

confidence: 99%

MiR-539-5p alleviates sepsis-induced acute lung injury by targeting ROCK1

Cao²,

Wan

et al. 2020

Folia Histochem Cytobiol.

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Introduction. Sepsis-induced acute lung injury (ALI) is an inflammatory process involved with simultaneous production of inflammatory cytokines and chemokines. In this study, we investigated the regulatory role of miR-539-5p in sepsis-induced ALI using a mouse model of cecal ligation puncture (CLP) and an in vitro model of primary murine pulmonary microvascular endothelial cells (MPVECs). Material and methods. Adult male C57BL/6 mice were intravenously injected with or without miR-539-5p agomir or scrambled control one week before CLP operation. MPVECs were transfected with miR-539-5p mimics or control mimics, followed by lipopolysaccharide (LPS) stimulation. ROCK1 was predicted and confirmed as a direct target of miR-539-5p using dual-luciferase reporter assay. In rescue experiment, MPVECs were co-transfected with lentiviral vector expressing ROCK1 (or empty vector) and miR-539-5p mimics 24 h before LPS treatment. The transcriptional activity of caspase-3, the apoptosis ratio, the levels of miR-539-5p, interleukin-1b (IL-1b), interleukin-6 (IL-6), and ROCK1 were assessed. Results. Compared to sham group, mice following CLP showed pulmonary morphological abnormalities, elevated production of IL-1b and IL-6, and increased caspase-3 activity and apoptosis ratio in the lung. In MPVECs, LPS stimulation resulted in a significant induction of inflammatory cytokine levels and apoptosis compared to untreated cells. The overexpression of miR-539-5p in septic mice alleviated sepsis-induced pulmonary injury, apoptosis, and inflammation. MiR-539-5p also demonstrated anti-apoptotic and anti-inflammatory effect in LPS-treated MPVECs. The upregulation of ROCK1 in MPVECs recovered miR-539-5p-suppressed caspase-3 activity and proinflammatory cytokine production. Conclusion. In conclusion, miR-539-5p alleviated sepsis-induced ALI via suppressing its downstream target ROCK1, suggesting a therapeutic potential of miR-539-5p for the management of sepsis-induced ALI.

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“…After incubating for 24 h at 37 °C, cells in the upper layer of the membrane were scraped, and cells in the lower layer were stained with crystal violet staining solution. Then, the cells were photographed and counted under a phase contrast microscope [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Abnormal bile acid-microbiota crosstalk promotes the development of hepatocellular carcinoma

Shen

et al. 2022

Hepatol Int

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Background Gut microbiota and microbe-derived metabolites are involved in the development of HCC. Bile acids (BAs) are the most important gut microbiota-modulated endogenous signaling molecules. Methods We tested serum bile acid levels and gut microbiome compositions in patients with HCC, chemical-induced HCC mouse models (DEN-HCC mice) and mouse orthotopic implanted liver tumor models with vancomycin treatment (vancomycin-treated mice). Then, we screened an important kind of HCC-related BAs, and verified its effect on the growth of HCC in vivo and in vitro. Results We found that the remarkably decreasing percentages of serum secondary BAs in the total bile acids of patients and DEN-HCC mice, especially, conjugated deoxycholic acids (DCA). The relative abundance of the bile salt hydrolase (BSH)-rich bacteria (Bifidobacteriales, Lactobacillales, Bacteroidales, and Clostridiales) was decreased in the feces of patients and DEN-HCC mice. Then, in vancomycin-treated mice, vancomycin treatment induced a reduction in the BSH-rich bacteria and promoted the growth of liver tumors. Similarly, the percentage of conjugated DCA after vancomycin treatment was significantly declined. We used a kind of conjugated DCA, Glyco-deoxycholic acid (GDCA), and found that GDCA remarkably inhibited the growth of HCC in vivo and in vitro. Conclusions We conclude that the remarkably decreasing percentages of serum conjugated DCA may be closely associated with HCC, which may be induced by the reducing gut BSH-rich bacteria. The mechanisms may be correlated with conjugated DCA directly inhibiting the growth and migration of HCC cells.

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MiR-539-5p negatively regulates migration of rMSCs induced by Bushen Huoxue decoction through targeting Wnt5a

Cited by 6 publications

References 33 publications

Recruitment of Mesenchymal Stem Cells to Damaged Sites by Plant-Derived Components

Recruitment of Mesenchymal Stem Cells to Damaged Sites by Plant-Derived Components

MiR-539-5p alleviates sepsis-induced acute lung injury by targeting ROCK1

Abnormal bile acid-microbiota crosstalk promotes the development of hepatocellular carcinoma

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